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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus USA300_FPR3757
locus tag: SAUSA300_RS04010 [old locus tag: SAUSA300_0743 ]
pan locus tag^?: SAUPAN002679000
symbol: SAUSA300_RS04010
pan gene symbol^?: hprK
synonym:
product: HPr kinase/phosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SAUSA300_RS04010 [old locus tag: SAUSA300_0743 ]
symbol: SAUSA300_RS04010
product: HPr kinase/phosphorylase
replicon: chromosome
strand: +
coordinates: 829446..830378
length: 933
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_007793 (829446..830378) NCBI
BioCyc: SAUSA300_RS04010 BioCyc
MicrobesOnline: see SAUSA300_0743

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
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361
421
481
541
601
661
721
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841
901
ATGTTAACGACAGAAAAACTAGTTGAAACATTAAAGTTAGATTTAATCGCTGGTGAAGAA
GGACTATCGAAGCCAATTAAAAATGCTGATATATCAAGACCGGGCTTAGAGATGGCAGGT
TATTTTTCACATTATGCGTCAGATAGAATACAACTATTAGGAACAACGGAACTATCGTTT
TACAATTTATTACCAGATAAGGATCGCGCAGGTCGTATGCGTAAACTATGCAGACCAGAA
ACGCCTGCAATTATTGTGACACGTGGATTGCAGCCACCAGAAGAATTAGTTGAAGCTGCA
AAAGAATTAAATACCCCACTTATAGTTGCTAAAGATGCGACTACAAGTTTAATGAGTCGC
TTAACAACGTTTTTAGAGCATGCACTTGCAAAGACGACATCTTTACATGGTGTTTTAGTA
GATGTTTACGGTGTTGGTGTACTAATTACCGGTGATTCAGGAATAGGTAAAAGTGAGACT
GCGTTGGAATTAGTTAAACGTGGGCATAGATTAGTAGCAGATGATAATGTAGAAATACGT
CAAATTAATAAAGATGAACTAATAGGGAAACCACCAAAGTTAATAGAACATCTATTAGAA
ATACGTGGACTAGGTATTATCAATGTTATGACTTTATTTGGCGCGGGTTCAATATTAACT
GAAAAACGAATTAGATTAAATATTAATTTGGAAAACTGGAACAAGCAAAAGTTATATGAC
CGCGTAGGTCTTAATGAAGAGACGCTAAGTATTTTAGATACTGAAATCACTAAAAAAACA
ATACCTGTAAGACCTGGTAGAAATGTTGCGGTAATTATTGAGGTCGCTGCAATGAACTAT
CGATTAAATATCATGGGCATTAACACGGCCGAAGAATTTAGTGAAAGATTAAATGAAGAA
ATTATCAAGAACAGTCATAAGAGTGAGGAGTAG
60
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933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: SAUSA300_RS04010 [old locus tag: SAUSA300_0743 ]
symbol: SAUSA300_RS04010
description: HPr kinase/phosphorylase
length: 310
theoretical pI: 6.27773
theoretical MW: 34481.7
GRAVY: -0.173226

⊟Function[edit | edit source]

reaction:
EC 2.7.4.-? ExPASy EC 2.7.11.-? ExPASy
ATP + HPr = ADP + P-Ser-HPr? P-Ser-HPr + phosphate = HPr + diphosphate?
TIGRFAM:
Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
and 3 more
HprK-related kinase B (TIGR04355; HMM-score: 23.5)
HprK-related kinase A (TIGR04352; HMM-score: 15)
SynChlorMet cassette protein ScmC (TIGR04249; HMM-score: 12.6)
TheSEED: see SAUSA300_0743
PFAM:
PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 251.6)
and 5 more
MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 134.3)
no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 26.7)
MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 21)
P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.3)
AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors: Mg²⁺
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00494
- TAT(Tat/SPI): 0.000283
- LIPO(Sec/SPII): 0.000768
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 446880968 NCBI
RefSeq: WP_000958224 NCBI
UniProt: see SAUSA300_0743

⊟Protein sequence[edit | edit source]

MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSHYASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKELNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSETALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSILTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVAAMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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