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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0728 [new locus tag: NWMN_RS04125 ]
  • pan locus tag?: SAUPAN002679000
  • symbol: hprK
  • pan gene symbol?: hprK
  • synonym:
  • product: HPr kinase/phosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0728 [new locus tag: NWMN_RS04125 ]
  • symbol: hprK
  • product: HPr kinase/phosphorylase
  • replicon: chromosome
  • strand: +
  • coordinates: 819936..820868
  • length: 933
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAACGACAGAAAAACTAGTTGAAACATTAAAGTTAGATTTAATCGCTGGTGAAGAA
    GGACTATCGAAGCCAATTAAAAATGCTGATATATCAAGACCGGGCTTAGAGATGGCAGGT
    TATTTTTCACATTATGCGTCAGATAGAATACAACTATTAGGAACAACGGAACTATCGTTT
    TACAATTTATTACCAGATAAGGATCGCGCAGGTCGTATGCGTAAACTATGCAGACCAGAA
    ACGCCTGCAATTATTGTGACACGTGGATTGCAGCCACCAGAAGAATTAGTTGAAGCTGCA
    AAAGAATTAAATACCCCACTTATAGTTGCTAAAGATGCGACTACAAGTTTAATGAGTCGC
    TTAACAACGTTTTTAGAGCATGCACTTGCAAAGACGACATCTTTACATGGTGTTTTAGTA
    GATGTTTACGGTGTTGGTGTACTAATTACCGGTGATTCAGGAATAGGTAAAAGTGAGACT
    GCGTTGGAATTAGTTAAACGTGGGCATAGATTAGTAGCAGATGATAATGTAGAAATACGT
    CAAATTAATAAAGATGAACTAATAGGGAAACCACCAAAGTTAATAGAACATCTATTAGAA
    ATACGTGGACTAGGTATTATCAATGTTATGACTTTATTTGGCGCGGGTTCAATATTAACT
    GAAAAACGAATTAGATTAAATATTAATTTGGAAAACTGGAACAAGCAAAAGTTATATGAC
    CGCGTAGGTCTTAATGAAGAGACGCTAAGTATTTTAGATACTGAAATCACTAAAAAAACA
    ATACCTGTAAGACCTGGTAGAAATGTTGCGGTAATTATTGAGGTCGCTGCAATGAACTAT
    CGATTAAATATCATGGGCATTAACACGGCCGAAGAATTTAGTGAAAGATTAAATGAAGAA
    ATTATCAAGAACAGTCATAAGAGTGAGGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0728 [new locus tag: NWMN_RS04125 ]
  • symbol: HprK
  • description: HPr kinase/phosphorylase
  • length: 310
  • theoretical pI: 6.27773
  • theoretical MW: 34481.7
  • GRAVY: -0.173226

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.4.-?  ExPASy    EC 2.7.11.-?  ExPASy
    ATP + HPr = ADP + P-Ser-HPr? P-Ser-HPr + phosphate = HPr + diphosphate?
  • TIGRFAM:
    Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
    and 3 more
    HprK-related kinase B (TIGR04355; HMM-score: 23.5)
    HprK-related kinase A (TIGR04352; HMM-score: 15)
    SynChlorMet cassette protein ScmC (TIGR04249; HMM-score: 12.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 251.6)
    and 5 more
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 134.3)
    no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 26.7)
    MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 21)
    P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00494
    • TAT(Tat/SPI): 0.000283
    • LIPO(Sec/SPII): 0.000768
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSHYASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKELNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSETALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSILTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVAAMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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