PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0743 [new locus tag: SAUSA300_RS04010 ]
• pan locus tag^?: SAUPAN002679000
• symbol: hprK
• pan gene symbol^?: hprK
• synonym:
• product: HPr kinase/phosphorylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0743 [new locus tag: SAUSA300_RS04010 ]
• symbol: hprK
• product: HPr kinase/phosphorylase
• replicon: chromosome
• strand: +
• coordinates: 829446..830378
• length: 933
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914551 NCBI
• RefSeq: YP_493445 NCBI
• BioCyc: see SAUSA300_RS04010
• MicrobesOnline: 1292258 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTTAACGACAGAAAAACTAGTTGAAACATTAAAGTTAGATTTAATCGCTGGTGAAGAA
  GGACTATCGAAGCCAATTAAAAATGCTGATATATCAAGACCGGGCTTAGAGATGGCAGGT
  TATTTTTCACATTATGCGTCAGATAGAATACAACTATTAGGAACAACGGAACTATCGTTT
  TACAATTTATTACCAGATAAGGATCGCGCAGGTCGTATGCGTAAACTATGCAGACCAGAA
  ACGCCTGCAATTATTGTGACACGTGGATTGCAGCCACCAGAAGAATTAGTTGAAGCTGCA
  AAAGAATTAAATACCCCACTTATAGTTGCTAAAGATGCGACTACAAGTTTAATGAGTCGC
  TTAACAACGTTTTTAGAGCATGCACTTGCAAAGACGACATCTTTACATGGTGTTTTAGTA
  GATGTTTACGGTGTTGGTGTACTAATTACCGGTGATTCAGGAATAGGTAAAAGTGAGACT
  GCGTTGGAATTAGTTAAACGTGGGCATAGATTAGTAGCAGATGATAATGTAGAAATACGT
  CAAATTAATAAAGATGAACTAATAGGGAAACCACCAAAGTTAATAGAACATCTATTAGAA
  ATACGTGGACTAGGTATTATCAATGTTATGACTTTATTTGGCGCGGGTTCAATATTAACT
  GAAAAACGAATTAGATTAAATATTAATTTGGAAAACTGGAACAAGCAAAAGTTATATGAC
  CGCGTAGGTCTTAATGAAGAGACGCTAAGTATTTTAGATACTGAAATCACTAAAAAAACA
  ATACCTGTAAGACCTGGTAGAAATGTTGCGGTAATTATTGAGGTCGCTGCAATGAACTAT
  CGATTAAATATCATGGGCATTAACACGGCCGAAGAATTTAGTGAAAGATTAAATGAAGAA
  ATTATCAAGAACAGTCATAAGAGTGAGGAGTAG
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  933
  

⊟Protein

⊟General

• locus tag: SAUSA300_0743 [new locus tag: SAUSA300_RS04010 ]
• symbol: HprK
• description: HPr kinase/phosphorylase
• length: 310
• theoretical pI: 6.27773
• theoretical MW: 34481.7
• GRAVY: -0.173226

⊟Function

• reaction:
  EC 2.7.4.-?  ExPASy    EC 2.7.11.-?  ExPASy
  ATP + HPr = ADP + P-Ser-HPr? P-Ser-HPr + phosphate = HPr + diphosphate?
• TIGRFAM:
  Signal transduction Regulatory functions Protein interactions HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
  Signal transduction Signal transduction PTS HPr(Ser) kinase/phosphatase (TIGR00679; EC 2.7.1.-; HMM-score: 388)
  and 3 more
  HprK-related kinase B (TIGR04355; HMM-score: 23.5)
  HprK-related kinase A (TIGR04352; HMM-score: 15)
  SynChlorMet cassette protein ScmC (TIGR04249; HMM-score: 12.6)
• TheSEED  :
  • HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)
  Carbohydrates Sugar alcohols Mannitol Utilization  HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)
  and 1 more
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory HPr catabolite repression system  HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)
• PFAM:
  PEP-carboxyk (CL0374) Hpr_kinase_C; HPr Serine kinase C-terminal domain (PF07475; HMM-score: 251.6)
  and 5 more
  MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 134.3)
  no clan defined PucR; Purine catabolism regulatory protein-like family (PF07905; HMM-score: 26.7)
  MurF-HprK_N (CL0365) DRTGG; DRTGG domain (PF07085; HMM-score: 21)
  P-loop_NTPase (CL0023) AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.3)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00494
  • TAT(Tat/SPI): 0.000283
  • LIPO(Sec/SPII): 0.000768
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161863 NCBI
• RefSeq: YP_493445 NCBI
• UniProt: Q2FIN3 UniProt

⊟Protein sequence

• MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSHYASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKELNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSETALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSILTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVAAMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hprK > lgt > SAUSA300_0745 > SAUSA300_0746 > trxB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications