PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS03950 [old locus tag: SAUSA300_0734 ]
• pan locus tag^?: SAUPAN002659000
• symbol: SAUSA300_RS03950
• pan gene symbol^?: comFA
• synonym:
• product: comf operon protein 1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS03950 [old locus tag: SAUSA300_0734 ]
• symbol: SAUSA300_RS03950
• product: comf operon protein 1
• replicon: chromosome
• strand: +
• coordinates: 814619..815701
• length: 1083
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (814619..815701) NCBI
• BioCyc: SAUSA300_RS03950 BioCyc
• MicrobesOnline: see SAUSA300_0734

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  361
  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  
  ATGGATAATGTAACAAGATATAAAATAACAGAGAGTTCGCAAAGTTCATCACAAGCATAT
  TATCATCTCTCATTTGAATTGTCGGAACAGCAGTCTTATGCCTCAGAACATATTGTTCGA
  GCCATTAGAAAGAGACAAACGATTTTGTTATATGCCGTAACAGGTGCAGGTAAGACAGAA
  ATGATGTTTCAAGGCATTCAATATGCAAGAATACAGGGAGATAATATAGCTATTGTGTCA
  CCACGTGTAGATGTTGTTGTAGAAATTAGTAAACGTATTAAAGACGCATTTCTTAATGAA
  GATATAGACATACTACACCAGCAATCAAGACAACAATTTGAAGGGCATTTTGTTGTATGC
  ACAGTGCATCAACTTTACCGATTCAAACAGCACTTTGATACTATTTTTATTGATGAAGTC
  GATGCCTTTCCTTTATCAATGGATAAAAATTTACAACAAGCATTGAAGTCATCTTCTAAA
  GTTGAACATGCAACAATTTATATGACAGCAACACCACCGAAACAACTTCTGTCAGAGATT
  CCCCACGAAAATATAATTAAATTGCCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCT
  AAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTA
  CAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATG
  ATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAG
  GATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTT
  ACTACGACAATATTAGAACGTGGATTTACAATGGCAAATTTGGATGTTGTTGTTATCGAT
  GCACATCAATATACTCAAGAGGCTTTAATACAAATTGCTGGACGTGTTGGACGAAAATTA
  GAATGTCCTACTGGAAAAGTATTGTTTTTTCATGAAGGGGTAAGTATGAATATGATTCAA
  GCTAAAAAAGAGATTCAAAGGATGAACAAATTAGCATTAAAAAGAGGTTGGATTGATGAA
  TAA
  60
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  1083
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS03950 [old locus tag: SAUSA300_0734 ]
• symbol: SAUSA300_RS03950
• description: comf operon protein 1
• length: 360
• theoretical pI: 9.83425
• theoretical MW: 42111.4
• GRAVY: -0.346111

⊟Function

• TIGRFAM:
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 50.8)
  and 5 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 28.8)
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 19.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 19.5)
  DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 18.8)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein YjeE (TIGR00150; HMM-score: 13.4)
• TheSEED: see SAUSA300_0734
• PFAM:
  P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 44.3)
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 44)
  and 10 more
  DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 25.4)
  Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 20.9)
  no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 19.1)
  P-loop_NTPase (CL0023) Helicase_RecD; Helicase (PF05127; HMM-score: 18.6)
  AAA_19; AAA domain (PF13245; HMM-score: 17.6)
  AAA_30; AAA domain (PF13604; HMM-score: 16.1)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.9)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 13.4)
  AAA_24; AAA domain (PF13479; HMM-score: 12.1)
  Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 10.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010697
  • TAT(Tat/SPI): 0.000822
  • LIPO(Sec/SPII): 0.000791
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 486198798 NCBI
• RefSeq: WP_001548033 NCBI
• UniProt: see SAUSA300_0734

⊟Protein sequence

• MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRKRQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDIDILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSKVEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNKKIQKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications