PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0705 [new locus tag: SA_RS04010 ]
• pan locus tag^?: SAUPAN002659000
• symbol: SA0705
• pan gene symbol^?: comFA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0705 [new locus tag: SA_RS04010 ]
• symbol: SA0705
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 803795..804697
• length: 903
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123512 NCBI
• RefSeq: NP_373960 NCBI
• BioCyc: see SA_RS04010
• MicrobesOnline: 102986 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  541
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  901
  
  ATGATGTTTCAAGGCATTCAATATGCAAGACGACAGGGAGATAATATAGCTATTGTGTCA
  CCGCGTGTAGATGTTGTTGTAGAAATTAGTAAACGTATTAAAGACGCATTTCTTAATGAA
  GATATAGACATACTACACCAGCAATCAAGTCAACAATTTGAAGGGCATTTTGTTGTATGC
  ACAGTACATCAACTTTACCGATTCAAACAGCACTTTGATACTATTTTTATTGATGAAGTC
  GATGCCTTTCCTTTATCAATGGATAAAAGTTTACAACAAGCATTGAAGTCATCTTCTAAA
  GTTGAACATGCAACGATTTATATGACAGCAACACCACCGAAACAACTTCTGTCAGAGATT
  CCCCACGAAAATATAATTAAATTGCCAGCTCGCTTTCATAAAAAATCACTTCCAGTTCCT
  AAATATCGTTATTTCAAACTTAATAATAATAAGATTCAGAAAATGTTATACCGAATTTTA
  CAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATG
  ATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAG
  GATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTT
  ACTACGACAATATTAGAACGTGGATTTACAATGGCAAATTTGGATGTTGTTGTTATCGAT
  GCACATCAATATACTCAAGAGGCTTTAATACAAATTGCTGGACGTGTTGGACGAAAATTA
  GAATGTCCTGCTGGAAAAGTATTGTTTTTTCATGAAGGAGTAAGTATGAATATGATTCAA
  GCTAAAAAAGAGATTCAAAGGATGAACAAATTAGCATTAAAAAGAGGTTGGATTGATGAA
  TAA
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⊟Protein

⊟General

• locus tag: SA0705 [new locus tag: SA_RS04010 ]
• symbol: SA0705
• description: hypothetical protein
• length: 300
• theoretical pI: 9.84873
• theoretical MW: 35159.6
• GRAVY: -0.292333

⊟Function

• TIGRFAM:
  CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 48.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 43.3)
  and 4 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 23.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.3)
  helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 20)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 10.3)
• TheSEED  :
  • ComF operon protein A, DNA transporter ATPase
  DNA Metabolism DNA uptake, competence Gram Positive Competence  ComF operon protein A, DNA transporter ATPase
  and 1 more
  DNA Metabolism DNA uptake, competence Late competence  ComF operon protein A, DNA transporter ATPase
• PFAM:
  P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 44.5)
  and 4 more
  no clan defined PrpR_N; Propionate catabolism activator (PF06506; HMM-score: 22)
  P-loop_NTPase (CL0023) Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 14.5)
  Glyco_hydro_tim (CL0058) Glyco_hydro_70; Glycosyl hydrolase family 70 (PF02324; HMM-score: 11.4)
  P-loop_NTPase (CL0023) Terminase_GpA; Phage terminase large subunit (GpA) (PF05876; HMM-score: 10.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001963
  • TAT(Tat/SPI): 0.00013
  • LIPO(Sec/SPII): 0.000631
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926427 NCBI
• RefSeq: NP_373960 NCBI
• UniProt: A0A0H3JL86 UniProt

⊟Protein sequence

• MMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDIDILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSKVEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPAGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0705 > SA0706 > SA0707

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications