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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02720 [old locus tag: SAUSA300_0508 ]
  • pan locus tag?: SAUPAN002288000
  • symbol: SAUSA300_RS02720
  • pan gene symbol?: mcsA
  • synonym:
  • product: excinuclease ABC subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02720 [old locus tag: SAUSA300_0508 ]
  • symbol: SAUSA300_RS02720
  • product: excinuclease ABC subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 567121..567609
  • length: 489
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGTGTGTCAAACTTGTGCTGAGGGACACCATCCGTGGAATCAAGCTAATGAACAACCT
    GAATATCAAGAACATCAAGATAATTTCGAAGAAGCATTTGTTGTTAAGCAAATTTTACAA
    CATTTAGCTACGAAACATGGAATTAATTTTCAAGAAGTAGCGTTTAAAGAAGAAAAACGT
    TGCCCATCATGTCATATGACTTTGAAAGATATTGCACATGTTGGTAAATTTGGGTGTGCT
    AATTGTTATGCAACATTTAAAGATGACATCATTGATATCGTCCGCAGAGTTCAAGGTGGA
    CAATTTGAGCACGTTGGAAAGACACCACATTCTTCACATAAAAAGATAGCTTTAAAGCGA
    AAAATCGAAGAAAAGAATGAATATTTGAAAAAACTTATTGAAATCCAAGATTTTGAGGAA
    GCAGCCATTGTTAGAGATGAAATTAAAGCACTAAAAGCTGAGAGTGAGGTGCAACATGAT
    GACGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    489

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS02720 [old locus tag: SAUSA300_0508 ]
  • symbol: SAUSA300_RS02720
  • description: excinuclease ABC subunit B
  • length: 162
  • theoretical pI: 6.42997
  • theoretical MW: 18710
  • GRAVY: -0.72037

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 14.8)
    Metabolism Transport and binding proteins Cations and iron carrying compounds sodium/hydrogen antiporter (TIGR00844; HMM-score: 12)
  • TheSEED: see SAUSA300_0508
  • PFAM:
    AAA_lid (CL0671) UVR; UvrB/uvrC motif (PF02151; HMM-score: 23.2)
    and 5 more
    Zn_Beta_Ribbon (CL0167) Cas12f1-like_TNB; Cas12f1-like, TNB domain (PF07282; HMM-score: 18.2)
    Zn_ribbon_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 17.8)
    RING (CL0229) C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain) (PF00130; HMM-score: 9.2)
    Multiheme_cytos (CL0317) Cytochrom_CIII; Class III cytochrome C family (PF02085; HMM-score: 8.5)
    Zn_Beta_Ribbon (CL0167) Zn_ribbon_6; Double zinc ribbon (PF07191; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9914
    • Cytoplasmic Membrane Score: 0.0038
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0046
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002846
    • TAT(Tat/SPI): 0.000231
    • LIPO(Sec/SPII): 0.000513
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVCQTCAEGHHPWNQANEQPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGKFGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIEIQDFEEAAIVRDEIKALKAESEVQHDDA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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