PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS02525 [old locus tag: SAUSA300_0470 ]
• pan locus tag^?: SAUPAN002231000
• symbol: SAUSA300_RS02525
• pan gene symbol^?: ksgA
• synonym:
• product: ribosomal RNA small subunit methyltransferase A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS02525 [old locus tag: SAUSA300_0470 ]
• symbol: SAUSA300_RS02525
• product: ribosomal RNA small subunit methyltransferase A
• replicon: chromosome
• strand: +
• coordinates: 529280..530173
• length: 894
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (529280..530173) NCBI
• BioCyc: SAUSA300_RS02525 BioCyc
• MicrobesOnline: see SAUSA300_0470

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTGGATAATAAAGATATTGCAACACCATCAAGAACGCGAGCGTTGTTAGATAAATAT
  GGCTTTAATTTTAAAAAAAGTTTAGGACAGAACTTTTTGATAGATGTGAATATCATTAAT
  AATATCATTGATGCAAGTGATATTGATGCACAAACTGGGGTGATTGAAATTGGTCCAGGC
  ATGGGGTCATTGACAGAACAATTGGCCAGACATGCTAAAAGAGTATTGGCATTTGAAATT
  GATCAACGTTTAATACCTGTATTAAATGATACACTATCACCTTATGATAATGTGACGGTG
  ATTAATGAAGATATTTTAAAAGCGAATATTAAAGAAGCTGTTGAAAATCATTTACAAGAT
  TGTGAAAAAATAATGGTTGTTGCAAACCTGCCGTACTATATTACGACGCCAATTTTATTA
  AATTTGATGCAACAAGATATACCAATTGATGGCTACGTGGTGATGATGCAAAAAGAAGTG
  GGCGAACGCTTAAATGCTGAAGTAGGTTCAAAAGCATATGGTTCGTTATCAATTGTCGTA
  CAATACTATACAGAGACTAGTAAAGTATTAACGGTACCTAAATCTGTATTTATGCCACCA
  CCTAATGTTGATTCAATAGTTGTAAAACTGATGCAGAGAACTGAACCGTTAGTAACAGTA
  GATAACGAGGAAGCATTCTTTAAGTTAGCAAAAGCAGCATTTGCACAAAGAAGAAAGACA
  ATTAACAATAACTATCAAAATTATTTTAAAGATGGTAAACAACACAAAGAAGTGATTTTA
  CAATGGTTGGAACAAGCAGGTATTGATCCAAGACGTCGCGGTGAAACGCTATCTATTCAA
  GATTTTGCTAAATTGTATGAAGAAAAGAAAAAATTCCCTCAATTAGAAAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS02525 [old locus tag: SAUSA300_0470 ]
• symbol: SAUSA300_RS02525
• description: ribosomal RNA small subunit methyltransferase A
• length: 297
• theoretical pI: 5.49072
• theoretical MW: 33737.6
• GRAVY: -0.280471

⊟Function

• reaction:
  EC 2.1.1.182?  ExPASy
  16S rRNA (adenine¹⁵¹⁸-N⁶/adenine¹⁵¹⁹-N⁶)-dimethyltransferase 4 S-adenosyl-L-methionine + adenine¹⁵¹⁸/adenine¹⁵¹⁹ in 16S rRNA = 4 S-adenosyl-L-homocysteine + N⁶-dimethyladenine¹⁵¹⁸/N⁶-dimethyladenine¹⁵¹⁹ in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 281.2)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 15.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.4)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 12.7)
• TheSEED: see SAUSA300_0470
• PFAM:
  NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 235.4)
  and 7 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 20.8)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 19.1)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 17.8)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 16.4)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 16)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 14.6)
  RsmJ; Ribosomal RNA large subunit methyltransferase D, RlmJ (PF04378; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003872
  • TAT(Tat/SPI): 0.000255
  • LIPO(Sec/SPII): 0.000268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SAUSA300_0470
• RefSeq: WP_000886500 NCBI
• UniProt: see SAUSA300_0470

⊟Protein sequence

• MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications