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⊟Summary[edit | edit source]
- pan ID?: SAUPAN002231000
- symbol?: ksgA
- synonym:
- description?: dimethyladenosine transferase
- dimethyladenosine transferase
- ribosomal RNA small subunit methyltransferase A
- 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA
- KsgA/Dim1 family 16S ribosomal RNA methyltransferase
- ribosomal RNA adenine dimethylase
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 2739870..2740781
- synteny block?: BlockID0015300
- occurrence?: in 100% of 34 strains
ksgA (rsmA) : ribosome small subunit methyltransferase A [1]
Ribosome small subunit methyltransferase A (alt: 16S rRNA adenosine(1529)- and adenosine(1530)-N-(6)-dimethyltransferase) iteratively attaches two methyl groups each to the adenosine nucleobase 6-positions of residues 1529 and 1530 in 16S ribosomal RNA (S. aureus numbering; adenosines 1518 and 1519 by E. coli numbering). RsmA is the only known ribosomal methyltransferase to act twice on the same nucleotide and the only known ribosomal methyltransferase to act on adjacent nucleotides. These modifications properly orient the ribosome decoding center and enhance translational fidelity.
- 16S rRNA : A (1529) → m6,6A (1529) , A (1530) → m6,6A (1530)
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0468
08BA02176:
C248_0548
11819-97:
MS7_0470 (ksgA)
6850:
RSAU_000430 (ksgA)
71193:
ST398NM01_0559
ECT-R 2:
ECTR2_433 (ksgA)
ED133:
SAOV_0515 (ksgA)
ED98:
SAAV_0440 (ksgA)
HO 5096 0412:
SAEMRSA15_04190
JH1:
SaurJH1_0527 (ksgA)
JH9:
SaurJH9_0514 (ksgA)
JKD6008:
SAA6008_00497 (ksgA)
JKD6159:
SAA6159_00444 (ksgA)
JSNZ:
JSNZ_000432 (rsmA)
LGA251:
SARLGA251_04270
M013:
M013TW_0464
MRSA252:
SAR0494 (ksgA)
MSHR1132:
SAMSHR1132_04370
MSSA476:
SAS0450 (ksgA)
Mu3:
SAHV_0490 (ksgA)
Mu50:
SAV0493 (ksgA)
MW2:
MW0448 (ksgA)
RF122:
SAB0442 (ksgA)
ST398:
SAPIG0559 (ksgA)
T0131:
SAT0131_00538
TCH60:
HMPREF0772_10027 (ksgA)
TW20:
SATW20_05610
USA300_TCH1516:
USA300HOU_0493 (ksgA)
VC40:
SAVC_02050 (ksgA)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
N315 MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
NCTC8325 MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
Newman MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
USA300_FPR3757 MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPG
************************************************************
COL MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
N315 MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
NCTC8325 MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
Newman IGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
USA300_FPR3757 MGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQD
:***********************************************************
COL CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
N315 CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
NCTC8325 CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
Newman CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
USA300_FPR3757 CEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVV
************************************************************
COL QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
N315 QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
NCTC8325 QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
Newman QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
USA300_FPR3757 QYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKT
************************************************************
COL INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
N315 INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
NCTC8325 INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
Newman INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
USA300_FPR3757 INNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN
*********************************************************
- ↑ Hasan Demirci, Frank Murphy, Riccardo Belardinelli, Ann C Kelley, V Ramakrishnan, Steven T Gregory, Albert E Dahlberg, Gerwald Jogl
Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function.
RNA: 2010, 16(12);2319-24
[PubMed:20962038] [WorldCat.org] [DOI] (I p)Alexander Golubev, Bulat Fatkhullin, Iskander Khusainov, Lasse Jenner, Azat Gabdulkhakov, Shamil Validov, Gulnara Yusupova, Marat Yusupov, Konstantin Usachev
Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution.
FEBS Lett: 2020, 594(21);3551-3567
[PubMed:32852796] [WorldCat.org] [DOI] (I p)Niklas C Stephan, Anne B Ries, Daniel Boehringer, Nenad Ban
Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA.
Nucleic Acids Res: 2021, 49(11);6389-6398
[PubMed:34086932] [WorldCat.org] [DOI] (I p)Adrián González-López, Daniel S D Larsson, Ravi Kiran Koripella, Brett N Cain, Martin Garcia Chavez, Paul J Hergenrother, Suparna Sanyal, Maria Selmer
Structures of the Staphylococcus aureus ribosome inhibited by fusidic acid and fusidic acid cyclopentane.
Sci Rep: 2024, 14(1);14253
[PubMed:38902339] [WorldCat.org] [DOI] (I e)