PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0470 [new locus tag: SAUSA300_RS02525 ]
• pan locus tag^?: SAUPAN002231000
• symbol: ksgA
• pan gene symbol^?: ksgA
• synonym: rsmA
• product: dimethyladenosine transferase

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0470 [new locus tag: SAUSA300_RS02525 ]
• symbol: ksgA
• product: dimethyladenosine transferase
• replicon: chromosome
• strand: +
• coordinates: 529280..530173
• length: 894
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913329 NCBI
• RefSeq: YP_493179 NCBI
• BioCyc: see SAUSA300_RS02525
• MicrobesOnline: 1291985 MicrobesOnline

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
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  841
  
  ATGTTGGATAATAAAGATATTGCAACACCATCAAGAACGCGAGCGTTGTTAGATAAATAT
  GGCTTTAATTTTAAAAAAAGTTTAGGACAGAACTTTTTGATAGATGTGAATATCATTAAT
  AATATCATTGATGCAAGTGATATTGATGCACAAACTGGGGTGATTGAAATTGGTCCAGGC
  ATGGGGTCATTGACAGAACAATTGGCCAGACATGCTAAAAGAGTATTGGCATTTGAAATT
  GATCAACGTTTAATACCTGTATTAAATGATACACTATCACCTTATGATAATGTGACGGTG
  ATTAATGAAGATATTTTAAAAGCGAATATTAAAGAAGCTGTTGAAAATCATTTACAAGAT
  TGTGAAAAAATAATGGTTGTTGCAAACCTGCCGTACTATATTACGACGCCAATTTTATTA
  AATTTGATGCAACAAGATATACCAATTGATGGCTACGTGGTGATGATGCAAAAAGAAGTG
  GGCGAACGCTTAAATGCTGAAGTAGGTTCAAAAGCATATGGTTCGTTATCAATTGTCGTA
  CAATACTATACAGAGACTAGTAAAGTATTAACGGTACCTAAATCTGTATTTATGCCACCA
  CCTAATGTTGATTCAATAGTTGTAAAACTGATGCAGAGAACTGAACCGTTAGTAACAGTA
  GATAACGAGGAAGCATTCTTTAAGTTAGCAAAAGCAGCATTTGCACAAAGAAGAAAGACA
  ATTAACAATAACTATCAAAATTATTTTAAAGATGGTAAACAACACAAAGAAGTGATTTTA
  CAATGGTTGGAACAAGCAGGTATTGATCCAAGACGTCGCGGTGAAACGCTATCTATTCAA
  GATTTTGCTAAATTGTATGAAGAAAAGAAAAAATTCCCTCAATTAGAAAATTAA
  60
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  894
  

⊟Protein

⊟General

• locus tag: SAUSA300_0470 [new locus tag: SAUSA300_RS02525 ]
• symbol: KsgA
• description: dimethyladenosine transferase
• length: 297
• theoretical pI: 5.49072
• theoretical MW: 33737.6
• GRAVY: -0.280471

⊟Function

• reaction:
  EC 2.1.1.182?  ExPASy
  16S rRNA (adenine¹⁵¹⁸-N⁶/adenine¹⁵¹⁹-N⁶)-dimethyltransferase 4 S-adenosyl-L-methionine + adenine¹⁵¹⁸/adenine¹⁵¹⁹ in 16S rRNA = 4 S-adenosyl-L-homocysteine + N⁶-dimethyladenine¹⁵¹⁸/N⁶-dimethyladenine¹⁵¹⁹ in 16S rRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 281.2)
  and 5 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 15.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.4)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 12.7)
• TheSEED  :
  • SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)
  Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182)
• PFAM:
  NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 244.7)
  and 5 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 22.5)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 18.2)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 16.3)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 12.6)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9987
  • Cytoplasmic Membrane Score: 0.0005
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0007
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003872
  • TAT(Tat/SPI): 0.000255
  • LIPO(Sec/SPII): 0.000268
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160474 NCBI
• RefSeq: YP_493179 NCBI
• UniProt: Q2FJE9 UniProt

⊟Additional information (user-provided)

⊟Protein sequence

• MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0469 > ksgA

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications