PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2612 [new locus tag: SAUSA300_RS14520 ]
• pan locus tag^?: SAUPAN006434000
• symbol: hisG
• pan gene symbol^?: hisG
• synonym:
• product: ATP phosphoribosyltransferase catalytic subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2612 [new locus tag: SAUSA300_RS14520 ]
• symbol: hisG
• product: ATP phosphoribosyltransferase catalytic subunit
• replicon: chromosome
• strand: -
• coordinates: 2839083..2839697
• length: 615
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914636 NCBI
• RefSeq: YP_495246 NCBI
• BioCyc: see SAUSA300_RS14520
• MicrobesOnline: 1294127 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTTAAGAATCGCCATAGCCAAAGGACGTCTAATGGATAGTTTAATTAACTATTTAGAT
  GTAATTGAATATACGACATTATCAGAAACATTAAAAAATAGAGAACGCCAATTATTATTA
  AGTGTAGATAATATTGAATGCATTTTAGTAAAAGGAAGTGACGTGCCAATCTATGTGGAA
  CAAGGAATGGCAGACATAGGCATTGTTGGTAGCGACATATTAGATGAGCGCCAATATAAT
  GTTAATAATTTGTTGAATATGCCTTTTGGAGCATGTCATTTTGCGGTTGCAGCGAAACCT
  GAAACGACCAATTATCGTAAAATCGCAACGAGTTATGTTCATACTGCTGAAACATATTTT
  AAATCAAAAGGTATTGATGTCGAATTGATTAAATTGAATGGCTCTGTTGAATTGGCCGGT
  GTTGTAGATATGGTAGACGGAATTGTCGACATCGTTCAAACAGGTACTACGCTAAAAGCG
  AACGGACTGGTTGAAAAGCAACATATTAGTGATATCAATGCAAGATTAATAACTAATAAA
  GCAGCTTATTTTAAAAAATCACAATTAATAGAGCAATTTATTCGCTCTTTGGAGGTGTCT
  ATTGCCAATGCTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2612 [new locus tag: SAUSA300_RS14520 ]
• symbol: HisG
• description: ATP phosphoribosyltransferase catalytic subunit
• length: 204
• theoretical pI: 5.323
• theoretical MW: 22596.9
• GRAVY: 0.0754902

⊟Function

• reaction:
  EC 2.4.2.17?  ExPASy
  ATP phosphoribosyltransferase 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family ATP phosphoribosyltransferase (TIGR00070; EC 2.4.2.17; HMM-score: 179.7)
• TheSEED  :
  • ATP phosphoribosyltransferase (EC 2.4.2.17)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  ATP phosphoribosyltransferase (EC 2.4.2.17)
  and 1 more
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  ATP phosphoribosyltransferase (EC 2.4.2.17)
• PFAM:
  PBP (CL0177) HisG; ATP phosphoribosyltransferase (PF01634; HMM-score: 153.9)
  and 1 more
  NMT1; NMT1/THI5 like (PF09084; HMM-score: 15.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00278
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000208
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162093 NCBI
• RefSeq: YP_495246 NCBI
• UniProt: Q2FDI2 UniProt

⊟Protein sequence

• MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDNIECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPETTNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELAGVVDMVDGIVDIVQTGTTLKANGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications