PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2609 [new locus tag: SAUSA300_RS14505 ]
• pan locus tag^?: SAUPAN006431000
• symbol: hisB
• pan gene symbol^?: hisB
• synonym:
• product: imidazoleglycerol-phosphate dehydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2609 [new locus tag: SAUSA300_RS14505 ]
• symbol: hisB
• product: imidazoleglycerol-phosphate dehydratase
• replicon: chromosome
• strand: -
• coordinates: 2836264..2836842
• length: 579
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914011 NCBI
• RefSeq: YP_495243 NCBI
• BioCyc: see SAUSA300_RS14505
• MicrobesOnline: 1294124 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTTATCAAAAACAACGAAACACAGCTGAAACGCAACTAAATATTTCAATATCTGAT
  GATCAGTCACCATCGCATATTAATACAGGTGTGGGCTTTTTAAATCATATGTTAACCTTG
  TTTACATTTCATAGCGGTCTGTCATTAAACATTGAGGCACAAGGTGATATTGACGTAGAT
  GATCACCACGTAACTGAAGATATCGGCATTGTCATTGGCCAATTGTTACTTGAAATGATT
  AAAGATAAAAAGCATTTCGTTCGTTATGGAACGATGTACATTCCAATGGATGAAACATTA
  GCACGTGTCGTTGTGGATATAAGTGGGCGCCCATACCTATCATTCAATGCATCATTAAGT
  AAAGAAAAAGTTGGTACGTTTGATACGGAGTTAGTAGAAGAATTTTTTAGAGCGGTCGTA
  ATCAATGCAAGATTAACAACGCATATTGATTTAATTCGTGGAGGTAATACACACCATGAA
  ATTGAAGCTATATTCAAAGCGTTTTCCCGTGCATTAGGCATAGCGCTAACTGCAACTGAT
  GATCAGCGTGTGCCGTCATCGAAAGGTGTGATTGAATGA
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  579
  

⊟Protein

• 2AE8
  PDB

Protein Data Bank: 2AE8

⊟General

• locus tag: SAUSA300_2609 [new locus tag: SAUSA300_RS14505 ]
• symbol: HisB
• description: imidazoleglycerol-phosphate dehydratase
• length: 192
• theoretical pI: 5.4988
• theoretical MW: 21456.2
• GRAVY: -0.0807292

⊟Function

• reaction:
  EC 4.2.1.19?  ExPASy
  Imidazoleglycerol-phosphate dehydratase D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O
• TIGRFAM:
• TheSEED  :
  • Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
  and 1 more
  Amino Acids and Derivatives Histidine Metabolism Histidine Biosynthesis in plants  Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)
• PFAM:
  S5 (CL0329) IGPD; Imidazoleglycerol-phosphate dehydratase (PF00475; HMM-score: 200.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.032907
  • TAT(Tat/SPI): 0.008802
  • LIPO(Sec/SPII): 0.000557
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160531 NCBI
• RefSeq: YP_495243 NCBI
• UniProt: Q2FDI5 UniProt

⊟Protein sequence

• MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFHSGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLARVVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHEIEAIFKAFSRALGIALTATDDQRVPSSKGVIE

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: hisIE < hisF < hisA < hisH < hisB < hisC < hisD < hisG < hisZ

⊟Regulation

• regulators: HisR* (repression) regulon, CodY (repression) regulon

  HisR*	(TF)	important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications