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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2159 [new locus tag: SAUSA300_RS11900 ]
- pan locus tag?: SAUPAN005606000
- symbol: SAUSA300_2159
- pan gene symbol?: —
- synonym:
- product: aldo/keto reductase family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2159 [new locus tag: SAUSA300_RS11900 ]
- symbol: SAUSA300_2159
- product: aldo/keto reductase family protein
- replicon: chromosome
- strand: -
- coordinates: 2336350..2337198
- length: 849
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914948 NCBI
- RefSeq: YP_494795 NCBI
- BioCyc: see SAUSA300_RS11900
- MicrobesOnline: 1293674 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGAATCATATTGAAATAAGTAAAGATGTAAAGATACCAGTATTAGGTTTCGGAGTATTT
CAAATTCCTCAAGAACAAACAGCAGAAGCGGTTAAAGAAGCAATTAAAGCGGGTTATAGA
CATATTGATACAGCACAAAGTTATTTAAATGAAACTGAAGTAGGTCAAGGTATTGAAGCT
TCAGGTATTGATAGAAGTGAACTATTCATTACGACTAAAGTATGGATTGAAAATGTTAAC
TATGAAGATACTATCAAATCAATCGAACGATCATTACAACGATTAAATTTGGACTATCTT
GATCTAGTGCTTATTCACCAACCTTACAATGACGTATATGGCTCATGGCGTGCATTAGAA
GAGTTAAAAGAAAATGGTAAAATTAAAGCAATCGGTGTATCTAACTTCGGCGTTGATCGA
ATTGTAGATTTAGGTATTCACAATCAAATTCAACCACAAGTGAACCAAATAGAAATTAAT
CCATTCCATCAACAAGAAGAACAAGTTGCAGCACTTCAACAAGAAAATGTTGTGGTAGAA
GCTTGGGCACCATTTGCAGAAGGTAAAAATCAATTATTCCAAAATCAATTGTTGCAGGCC
ATTGCAGATAAATACAACAAATCAATTGCACAAGTTATTTTACGCTGGTTAGTAGAACGT
GACATTGTTGTACTCGCAAAATCAGTGAATCCAGAACGTATGGCTCAAAACTTAGATATA
TTTGATTTTGAACTTACAGAAGAAGATAAACAACAAATTGCGACATTAGAAGAAAGTAAT
AGCCAATTTTTCTCACATGCTGATCCAGAGATGATTAAAGCACTTACAAGCAGAGAATTA
GATGTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2159 [new locus tag: SAUSA300_RS11900 ]
- symbol: SAUSA300_2159
- description: aldo/keto reductase family protein
- length: 282
- theoretical pI: 4.38261
- theoretical MW: 32202
- GRAVY: -0.336525
⊟Function[edit | edit source]
- reaction: EC 1.1.1.218? ExPASyMorphine 6-dehydrogenase Morphine + NAD(P)+ = morphinone + NAD(P)H
- TIGRFAM: voltage-dependent potassium channel beta subunit (TIGR01293; HMM-score: 47.3)
- TheSEED :
- Oxidoreductase of aldo/keto reductase family, subgroup 1
- PFAM: no clan defined Aldo_ket_red; Aldo/keto reductase family (PF00248; HMM-score: 180.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.105534
- TAT(Tat/SPI): 0.007155
- LIPO(Sec/SPII): 0.001178
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDTAQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDLVLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEINPFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLVERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALTSRELDV
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_1657 (ackA) acetate kinase [1] (data from MRSA252) SAUSA300_2142 (asp23) alkaline shock protein 23 [1] (data from MRSA252) SAUSA300_0570 (eutD) phosphotransacetylase [1] (data from MRSA252) SAUSA300_1080 (ftsZ) cell division protein FtsZ [1] (data from MRSA252) SAUSA300_0756 (gap) glyceraldehyde-3-phosphate dehydrogenase, type I [1] (data from MRSA252) SAUSA300_2104 (glmS) glucosamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) SAUSA300_1362 (hup) DNA-binding protein HU [1] (data from MRSA252) SAUSA300_1640 (icd) isocitrate dehydrogenase [1] (data from MRSA252) SAUSA300_0996 (lpdA) dihydrolipoamide dehydrogenase [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_0220 (pflB) formate acetyltransferase [1] (data from MRSA252) SAUSA300_0523 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_2189 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) SAUSA300_0015 (rplI) 50S ribosomal protein L9 [1] (data from MRSA252) SAUSA300_2172 (rplM) 50S ribosomal protein L13 [1] (data from MRSA252) SAUSA300_2185 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_2177 (rplQ) 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_1603 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_1149 (rpsB) 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_2198 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_2171 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_1545 (rpsT) 30S ribosomal protein S20 [1] (data from MRSA252) SAUSA300_0737 (secA) preprotein translocase subunit SecA [1] (data from MRSA252) SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_0355 acetyl-CoA acetyltransferase [1] (data from MRSA252) SAUSA300_0658 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_0995 branched-chain alpha-keto acid dehydrogenase subunit E2 [1] (data from MRSA252) SAUSA300_1652 hypothetical protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)