PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1929 [new locus tag: SAUSA300_RS10585 ]
• pan locus tag^?: SAUPAN005046000
• symbol: SAUSA300_1929
• pan gene symbol^?: —
• synonym:
• product: phi77 ORF004-like protein phage tail component

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1929 [new locus tag: SAUSA300_RS10585 ]
• symbol: SAUSA300_1929
• product: phi77 ORF004-like protein phage tail component
• replicon: chromosome
• strand: -
• coordinates: 2095297..2096781
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914637 NCBI
• RefSeq: YP_494580 NCBI
• BioCyc: see SAUSA300_RS10585
• MicrobesOnline: 1293444 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATAGACACTATTAAAGTGAACAACAAAACAATTCCTTGGTTGTATGTCGAAAGAGGG
  TTTGAAATACCCTCTTTTAATTATGTTTTAAAAACTGAAAATGTAGATGGACGTTCGGGG
  TCTATATATAAAGGGCGTAGGCTTGAATCTTATAGTTTTGATATACCTTTGGTGGTACGT
  AATGACTATTTATCTCACAACGGCTTTAAAACACATGATGACGTCTTGAATGAATTAGTA
  AAGTTTTTTAACTACGAGGAACAAGTTAAATTACAATTCAAATCTAAAGATTGGTACTGG
  AACGCTTATTTTGAAGGACCAATAAAGCTGCACAAAGAATTTGCAATACCTGTTAAGTTC
  ACTATCAAAGTAGTACTAACAGACCCTTACAAATATTCAGTAACAGGATATAAAAATACT
  GCGATTTCAGACCAAGTTTCAGTTGTAAATAGTGGGACTGCTGACACTCCTTTAATTGTT
  GAAGCCCGAGCAATTAAACCATCTAGTTACTTTATGATCACTAAAAATGATGAAGATTAT
  TTTATGGTTGGTGATGATGAGGTAACCAAAGAAGTTAAGGATTACATGCCTCCTGTTTAT
  CATAGTGAGTTTCGTGATTTCAAAGGTTGGACTAAGATGATTACTGAAGATATTCCAAGT
  AATGATTTAGGTGGTAAAGTCGGCGGTGACTTTGTGATATCCAATCTTGGCGAAGGATAT
  AAAGCAACTAATTTTTCTGATGCAAAAGGTTGGGTTGGTGCTGGCACGAAACGAGGGCTC
  CCTAAAGCGATGACAGATTTTCAAATTACCTATAAATGTATTGTTGAACAAAAAGGTAAA
  GGTGCCGGAAGAACAGCACAACATATTTATGATAGTGATGGTAAGTTACTTGCTTCTATT
  GGTTATGAAAATAAATATCATGATAGAAAAATAGGACATATTGTTGTTACGTTGTATAAC
  CAAAAAGGAGACCCCAAAAAGATATACGACTATCAGAATAAACCGATAATGTATAACTTG
  GACAGAATCGTTGTTTATATGCGGCTCAGAAGAGTAGGTAATAAATTTTCTATTAAAACT
  TGGAAATTTGATCACATTAAAGACCCAGATAGACGTAAACCTATTGATATGGATGAGAAA
  GAGTGGATAGATGGCGGTAAGTTTTATCAGCGTCCAGCTTCTATCATAGCTATCTATAGT
  GCGAAGTATAATGGTTATAAGTGGATGGAGATGAATGGATTAGGTTCATTCAATACGGAG
  ATTCTACCAAAACCGAAAGGCGCAAGGGATGTCATTATACAAAAAGGTGATTTAGTAAAA
  ATAGATATGCAAGCAAAAAGTGTTGTCATCAATGAGGAACCAATGTTGAGCGAGAAATCG
  TTTGGAAGTAATTATTTCAATGTTGATTCTGGGTACAGTGAATTAATCATACAACCTGAA
  AACGTCTTTGATACGACGGTTAAATGGCAAGATAGATATTTATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1929 [new locus tag: SAUSA300_RS10585 ]
• symbol: SAUSA300_1929
• description: phi77 ORF004-like protein phage tail component
• length: 494
• theoretical pI: 9.04655
• theoretical MW: 56998.5
• GRAVY: -0.547166

⊟Function

• TIGRFAM:
• TheSEED  :
  • Bacteriophage
• PFAM:
  no clan defined SPP1_Dit; Siphovirus-type tail component, C-terminal domain (PF22768; HMM-score: 44.7)
  and 2 more
  Phage_barrel (CL0504) Sipho_tail; Phage tail protein RIFT-related domain (PF05709; HMM-score: 29.3)
  no clan defined Sterile; Male sterility protein (PF03015; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0028
  • Cytoplasmic Membrane Score: 0.0076
  • Cell wall & surface Score: 0.0009
  • Extracellular Score: 0.9887
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00513
  • TAT(Tat/SPI): 0.00019
  • LIPO(Sec/SPII): 0.001054
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162094 NCBI
• RefSeq: YP_494580 NCBI
• UniProt: A0A0H2XK01 UniProt

⊟Protein sequence

• MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGSIYKGRRLESYSFDIPLVVRNDYLSHNGFKTHDDVLNELVKFFNYEEQVKLQFKSKDWYWNAYFEGPIKLHKEFAIPVKFTIKVVLTDPYKYSVTGYKNTAISDQVSVVNSGTADTPLIVEARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDIPSNDLGGKVGGDFVISNLGEGYKATNFSDAKGWVGAGTKRGLPKAMTDFQITYKCIVEQKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNKPIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRPASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVINEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1927 < SAUSA300_1928 < SAUSA300_1929 < SAUSA300_1930

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications