PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1491 [new locus tag: SAUSA300_RS08140 ]
• pan locus tag^?: SAUPAN004107000
• symbol: SAUSA300_1491
• pan gene symbol^?: pepQ2
• synonym:
• product: proline dipeptidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1491 [new locus tag: SAUSA300_RS08140 ]
• symbol: SAUSA300_1491
• product: proline dipeptidase
• replicon: chromosome
• strand: -
• coordinates: 1645001..1646062
• length: 1062
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913040 NCBI
• RefSeq: YP_494186 NCBI
• BioCyc: see SAUSA300_RS08140
• MicrobesOnline: 1293006 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAGCAGAATAACACAAGTGCATCGTATTTTAGAACAAAAACATTTAGATGCGATTATC
  ATTCTTTCGGATTACAATAGAAGATATTTATCAGGTTTTACTGGTACCAGTGGAGCACTT
  ATCATATCTAAAGATAAACAATATTTGATAACAGACTTTAGATATATTGATCAAGCAACT
  AAGCAGGCGCCAAATTATGAAATTATTAATCGTAAATCTACTATTATTGGTGAGATTAAA
  GAATTGCTACACCAAGAAAATTTTGAAAATGTAGGTTTTGAGGGGCATCATGTAAGTTAT
  GATACATACCTTGAATTAAATAAAAGCCGTATATCATTAATAAGCATTTCTAATACTGTA
  GATAAAATTAGAGACGTCAAAGATGCTGACGAAATTGCTTTAATTCAAAAAGCAGCTAAT
  ATTGTTGATGAAACATATGAATATATTTTAACTGTTGTAAAAGCAGGCATGACTGAAAAA
  GAATTAAAGGCAATATTAGAAAGCAAAATGCTAGAATTAGGAGCAGATGGACCATCATTC
  GATACGATTGTAGCATCTGGTCATAGAGGTGCATTACCACATGGTGTTGCAAGTGATAAA
  ATTATTGAAAAAGGCGACATGATTACATTAGATTTTGGCGCGTATTATAACGGCTATTGT
  TCAGATATTACTAGAACATTTGCTATTGGAGAACCAGATCCTAAACTGAAAGAAATATAT
  CAAATAGTACTTGAATCTCAAATGAAAGCAATTAATGAGATTAGACCTGGCATGACTGGT
  GCAGAAGCTGATGCCATTTCAAGAAACTATTTAGAGTCAAAAGGGTATGGAAAAGAATTT
  GGACATTCACTAGGACATGGTATTGGTTTAGAAATCCATGAAGGGCCAATGCTGGCTCGT
  ACGATACAAGATAAACTTCAAGTTAACAACTGTGTTACAGTAGAACCTGGTGTTTATATA
  GAAGGTTTGGGCGGTATAAGAATAGAAGATGATATTTTAATTACAGAAAATGGTTGTCAA
  GTCTTTACTAAATGCACAAAAGACCTTATAGTTTTAACATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1491 [new locus tag: SAUSA300_RS08140 ]
• symbol: SAUSA300_1491
• description: proline dipeptidase
• length: 353
• theoretical pI: 5.06349
• theoretical MW: 39337.6
• GRAVY: -0.207932

⊟Function

• reaction:
  EC 3.4.13.9?  ExPASy
  Xaa-Pro dipeptidase Hydrolysis of Xaa-|-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair methionine aminopeptidase, type I (TIGR00500; EC 3.4.11.18; HMM-score: 100.5)
  and 3 more
  ectoine utilization protein EutD (TIGR02993; EC 3.-.-.-; HMM-score: 64.2)
  Genetic information processing Protein fate Protein modification and repair methionine aminopeptidase, type II (TIGR00501; EC 3.4.11.18; HMM-score: 40.1)
  Unknown function General DNA-binding protein, 42 kDa (TIGR00495; HMM-score: 15.1)
• TheSEED  :
  • Xaa-Pro aminopeptidase (EC 3.4.11.9) YpdF-like
  Protein Metabolism Protein degradation Protein degradation  Aminopeptidase YpdF (MP-, MA-, MS-, AP-, NP- specific)
• PFAM:
  no clan defined Peptidase_M24; Metallopeptidase family M24 (PF00557; HMM-score: 232)
  and 1 more
  AMP_N-like (CL0356) Creatinase_N; Creatinase/Prolidase N-terminal domain (PF01321; HMM-score: 86.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.016788
  • TAT(Tat/SPI): 0.017013
  • LIPO(Sec/SPII): 0.001153
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160808 NCBI
• RefSeq: YP_494186 NCBI
• UniProt: A0A0H2XHB5 UniProt

⊟Protein sequence

• MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISKDKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDTYLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEKELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNGYCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGYGKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILITENGCQVFTKCTKDLIVLT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: efp < SAUSA300_1491

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications