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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1473 [new locus tag: SAUSA300_RS08040 ]
  • pan locus tag?: SAUPAN004079000
  • symbol: nusB
  • pan gene symbol?: nusB
  • synonym:
  • product: transcription antitermination protein NusB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1473 [new locus tag: SAUSA300_RS08040 ]
  • symbol: nusB
  • product: transcription antitermination protein NusB
  • replicon: chromosome
  • strand: -
  • coordinates: 1627942..1628331
  • length: 390
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAGTCGTAAAGAATCCCGAGTGCAAGCTTTTCAAACTTTATTTCAATTAGAAATGAAG
    GACAGTGATTTAACGATAAATGAAGCGATAAGCTTTATTAAAGACGATAATCCAGATTTA
    GACTTCGAATTTATTCATTGGCTAGTTTCTGGCGTTAAAGGTCACGAACCTGTATTAGAC
    GAGACAATTAGTCCTTATTTAAAAGATTGGACTATTGCACGTTTATTAAAAACGGATCGT
    ATTATTTTAAGAATGGCAACATATGAAATATTACACAGTGATACACCTGCTAAAGTCGTA
    ATGAATGAAGCAGTTGAATTAACAAAACAATTCAGTGATGATGATCATTATAAATTTATA
    AATGGTGTATTGAGTAATATAAAAAAATAA
    60
    120
    180
    240
    300
    360
    390

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1473 [new locus tag: SAUSA300_RS08040 ]
  • symbol: NusB
  • description: transcription antitermination protein NusB
  • length: 129
  • theoretical pI: 4.97329
  • theoretical MW: 15003
  • GRAVY: -0.327907

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Transcription Transcription factors transcription antitermination factor NusB (TIGR01951; HMM-score: 135)
    and 1 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 27)
  • TheSEED  :
    • Transcription termination protein NusB
    RNA Metabolism Transcription Transcription factors bacterial  Transcription termination protein NusB
  • PFAM:
    NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 116.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003146
    • TAT(Tat/SPI): 0.000273
    • LIPO(Sec/SPII): 0.000278
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEFIHWLVSGVKGHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMNEAVELTKQFSDDDHYKFINGVLSNIKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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