PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1475 [new locus tag: SAUSA300_RS08050 ]
• pan locus tag^?: SAUPAN004081000
• symbol: accC
• pan gene symbol^?: accC
• synonym:
• product: acetyl-CoA carboxylase biotin carboxylase subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1475 [new locus tag: SAUSA300_RS08050 ]
• symbol: accC
• product: acetyl-CoA carboxylase biotin carboxylase subunit
• replicon: chromosome
• strand: -
• coordinates: 1628768..1630123
• length: 1356
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913088 NCBI
• RefSeq: YP_494171 NCBI
• BioCyc: see SAUSA300_RS08050
• MicrobesOnline: 1292990 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAAAGGTTTTAATTGCAAACCGCGGTGAAATCGCAGTTAGGATTATTCGCGCTTGT
  CGTGATTTAGGCATCCAAACTGTTGCAATCTATTCTGAAGGGGATAAAGATGCGCTACAT
  ACTCAAATTGCTGATGAAGCATATTGCGTAGGTCCCACTTTGTCTAAAGATTCATATTTA
  AATATTCCGAACATCTTATCTATTGCAACTTCTACAGGTTGTGATGGCGTTCATCCGGGT
  TATGGCTTTTTAGCTGAAAATGCTGATTTTGCAGAATTATGCGAAGCATGCCAATTGAAG
  TTCATTGGACCAAGTTATCAATCTATCCAAAAAATGGGTATCAAAGATGTTGCTAAGGCA
  GAAATGATCAAAGCCAATGTTCCAGTTGTTCCTGGTAGTGACGGTTTAATGAAAGACGTC
  TCAGAAGCTAAGAAAATCGCCAAAAAAATTGGCTATCCGGTCATCATTAAAGCTACTGCT
  GGCGGTGGCGGAAAAGGTATCCGTGTTGCTCGTGATGAAAAAGAACTTGAAACTGGCTTC
  CGAATGACAGAACAAGAAGCTCAAACTGCATTTGGTAATGGTGGACTTTATATGGAGAAA
  TTCATCGAAAACTTCCGCCATATTGAAATCCAAATTGTTGGGGACAGCTATGGTAATGTA
  ATTCATTTAGGAGAACGTGATTGTACAATTCAAAGACGTATGCAGAAATTAGTGGAAGAA
  GCACCTTCCCCAATTTTAGATGATGAAACACGTCGTGAAATGGGAAATGCCGCAGTTCGT
  GCAGCGAAAGCTGTAAATTATGAAAATGCGGGAACAATTGAGTTTATATATGATTTAAAT
  GATAATAAATTTTATTTTATGGAAATGAATACACGTATTCAAGTAGAACATCCTGTAACT
  GAAATGGTAACAGGAATTGATTTAGTTAAATTACAATTACAAGTTGCTATGGGTGACGTG
  TTACCGTATAAACAAGAAGATATTAAATTAACAGGACACGCAATTGAATTTAGAATTAAT
  GCTGAAAATCCTTACAAGAACTTTATGCCATCACCAGGTAAAATTGAGCAATATCTTGCA
  CCAGGTGGATATGGTGTTCGAATAGAGTCAGCATGTTATACTAATTATACGATACCGCCA
  TATTATGATTCGATGGTAGCGAAATTAATCATACATGAACCGACACGAGATGAAGCGATT
  ATGGCTGGCATTCGTGCACTAAGTGAATTTGTGGTTCTTGGTATTGATACAACTATTCCA
  TTCCATATTAAATTATTGAATAACGATATATTTAGAAGCGGTAAATTTAATACAAACTTT
  TTAGAGCAAAATAGCATTATGAATGATGAAGGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1475 [new locus tag: SAUSA300_RS08050 ]
• symbol: AccC
• description: acetyl-CoA carboxylase biotin carboxylase subunit
• length: 451
• theoretical pI: 5.14581
• theoretical MW: 50049
• GRAVY: -0.211308

⊟Function

• reaction:
  EC 6.4.1.2?  ExPASy
  Acetyl-CoA carboxylase ATP + acetyl-CoA + HCO₃(-) = ADP + phosphate + malonyl-CoA
• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 738.1)
  and 11 more
  Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 551.1)
  Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 535.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 64)
  alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 43.6)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 41.9)
  lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 38.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 29)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 26.3)
  Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 26)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 20.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme gamma-F420-2:alpha-L-glutamate ligase (TIGR04443; EC 6.3.2.32; HMM-score: 12.9)
• TheSEED  :
  • Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)
  Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)
  and 1 more
  Miscellaneous Miscellaneous - no subcategory EC 6.3.4.- Ligases that form carbon-nitrogen bonds  Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)
• PFAM:
  ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 273.9)
  and 11 more
  PreATP-grasp (CL0483) Biotin_carb_N; Biotin carboxylase, N-terminal domain (PF00289; HMM-score: 152.3)
  Hybrid (CL0105) Biotin_carb_C; Biotin carboxylase C-terminal domain (PF02785; HMM-score: 127.9)
  ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 39.6)
  Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 34.4)
  ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 31.3)
  ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 26.4)
  RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 24.9)
  GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 14.8)
  ATPgrasp_ST; Sugar-transfer associated ATP-grasp (PF14397; HMM-score: 14.3)
  Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 13.8)
  ATP-grasp (CL0179) ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022724
  • TAT(Tat/SPI): 0.000898
  • LIPO(Sec/SPII): 0.008517
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160215 NCBI
• RefSeq: YP_494171 NCBI
• UniProt: A0A0H2XG70 UniProt

⊟Protein sequence

• MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIADEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFIGPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATAGGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYGNVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFIYDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHAIEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIHEPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDEG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1470 < xseB < xseA < nusB < SAUSA300_1474 < accC < accB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications