PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1417 [new locus tag: SAUSA300_RS07730 ]
• pan locus tag^?: SAUPAN001473000
• symbol: SAUSA300_1417
• pan gene symbol^?: —
• synonym:
• product: phiSLT ORF 175-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1417 [new locus tag: SAUSA300_RS07730 ]
• symbol: SAUSA300_1417
• product: phiSLT ORF 175-like protein
• replicon: chromosome
• strand: -
• coordinates: 1579290..1579826
• length: 537
• essential: unknown

⊟Accession numbers

• Gene ID: 3913324 NCBI
• RefSeq: YP_494114 NCBI
• BioCyc: see SAUSA300_RS07730
• MicrobesOnline: 1292932 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGACTAATACATTAACAATTGATCAGTTACAAGAGTTATTACAAATACAAAAGGAGTTC
  GACGATAGAATACCAACGCTGAACTTACGAGATAGCAAAATAGCATATGTAGTTGAATTC
  TTTGAATGGTTTAATACATTGGAAACGTTTAAGAACTGGAAGAAGAAACCAGGTAAGCCG
  TTAGATGTTCAGTTAGATGAATTAGCGGACATGTTGGCGTTTGGATTGAGTATTGCGAAT
  CAAGTAGGAGTGTCATCAGAAGAGATAAAAGAAGCGATTGAATCAAGTTTTAAAAATAGT
  GAATTTCACAACATGTTTAATTTTAAAGATAAAGAATTTGCTCAAGACGCAGTTGTTAGC
  ACACCACAGATAATATTCAAAGAATTTTATCCTGACCAATTGGCAATTGTAATAGCGATA
  GACATAGCTTTCAACTTATATTCTATCGACCAGCTCATTGACGCATACAAAAAGAAAATG
  AAAAGGAATCATGAAAGACAAGATGGAACAGCAGACGCAGGAAAAGGATACGTGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  537
  

⊟Protein

⊟General

• locus tag: SAUSA300_1417 [new locus tag: SAUSA300_RS07730 ]
• symbol: SAUSA300_1417
• description: phiSLT ORF 175-like protein
• length: 178
• theoretical pI: 4.59307
• theoretical MW: 20585.3
• GRAVY: -0.363483

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 13.1)
• TheSEED  :
  • Dimeric dUTPase (EC 3.6.1.23)
  Nucleosides and Nucleotides Detoxification Housecleaning nucleoside triphosphate pyrophosphatases  Dimeric dUTPase (EC 3.6.1.23)
• PFAM:
  MazG (CL0231) dUTPase_2; dUTPase (PF08761; HMM-score: 66.7)
  and 2 more
  MazG-like; MazG-like family (PF12643; HMM-score: 20.7)
  DUF550; Protein of unknown function (DUF550) (PF04447; HMM-score: 16.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000914
  • TAT(Tat/SPI): 0.000164
  • LIPO(Sec/SPII): 0.000289
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160992 NCBI
• RefSeq: YP_494114 NCBI
• UniProt: A0A0H2XGB9 UniProt

⊟Protein sequence

• MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWFNTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKNSEFHNMFNFKDKEFAQDAVVSTPQIIFKEFYPDQLAIVIAIDIAFNLYSIDQLIDAYKKKMKRNHERQDGTADAGKGYV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1411 < SAUSA300_1412 < SAUSA300_1413 < SAUSA300_1414 < SAUSA300_1415 < SAUSA300_1416 < SAUSA300_1417 < SAUSA300_1418 < SAUSA300_1419

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications