PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1012 [new locus tag: NWMN_RS05715 ]
• pan locus tag^?: SAUPAN001473000
• symbol: NWMN_1012
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1012 [new locus tag: NWMN_RS05715 ]
• symbol: NWMN_1012
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1113597..1114133
• length: 537
• essential: unknown

⊟Accession numbers

• Gene ID: 5330616 NCBI
• RefSeq: YP_001332046 NCBI
• BioCyc:
• MicrobesOnline: 3706563 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  
  ATGACTAACACATTAACAATTGATCAGTTACAAGAGTTATTACAAATACAAAAGGAGTTC
  GACGATAGAATACCAACGCTGAACTTACGAGATAGCAAAATAGCATATGTAGTTGAATTC
  TTTGAATGGTTTAATACATTGGAAACGTTTAAGAACTGGAAGAAGAAACCAGGTAAGCCG
  TTAGACGTACAACTTGATGAATTAGCTGACATGTTGGCGTTTGGATTGAGTATTGCGAAT
  CAAGTAGGAGTGTCATCAGAAGAGATAAAAGAAGCGATTGAATCAAGTTTTAAAGATACA
  GAATTTCACAAAATGTTTAATTTTAAAGATAAAGAATTTGCTCAAGACGCAGTTGTTAGT
  ACACCACAGATAATATTCAAAGAATTTTATCCCGACCAACAAGCAATTGTTATAGTGATA
  GACATAGCTTACAACTTATATTCTATCGACCAACTCATTGACGCATACAAAAAGAAAATG
  AAAAGGAATCATGAAAGACAAGATGGAACAGCAGACGCAGGAAAAGGATACGTGTAA
  60
  120
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  537
  

⊟Protein

⊟General

• locus tag: NWMN_1012 [new locus tag: NWMN_RS05715 ]
• symbol: NWMN_1012
• description: hypothetical protein
• length: 178
• theoretical pI: 4.6081
• theoretical MW: 20673.4
• GRAVY: -0.41573

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 12.9)
• TheSEED: data available for USA300_FPR3757
• PFAM:
  MazG (CL0231) dUTPase_2; dUTPase (PF08761; HMM-score: 68.2)
  and 2 more
  MazG-like; MazG-like family (PF12643; HMM-score: 20.6)
  DUF550; Protein of unknown function (DUF550) (PF04447; HMM-score: 15.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000914
  • TAT(Tat/SPI): 0.000164
  • LIPO(Sec/SPII): 0.000289
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221224 NCBI
• RefSeq: YP_001332046 NCBI
• UniProt: A0A0H3K6Q8 UniProt

⊟Protein sequence

• MTNTLTIDQLQELLQIQKEFDDRIPTLNLRDSKIAYVVEFFEWFNTLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQVGVSSEEIKEAIESSFKDTEFHKMFNFKDKEFAQDAVVSTPQIIFKEFYPDQQAIVIVIDIAYNLYSIDQLIDAYKKKMKRNHERQDGTADAGKGYV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications