PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1328 [new locus tag: SAUSA300_RS07240 ]
• pan locus tag^?: SAUPAN003890000
• symbol: SAUSA300_1328
• pan gene symbol^?: norB
• synonym:
• product: putative drug transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1328 [new locus tag: SAUSA300_RS07240 ]
• symbol: SAUSA300_1328
• product: putative drug transporter
• replicon: chromosome
• strand: -
• coordinates: 1488474..1489865
• length: 1392
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914312 NCBI
• RefSeq: YP_494025 NCBI
• BioCyc: see SAUSA300_RS07240
• MicrobesOnline: 1292843 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGGAAAAGCCGTCAAGAGAGGCATTTGAAGGCAATAATAAGTTGTTAATAGGAATTGTT
  CTAAGTGTAATAACGTTTTGGCTATTTGCACAATCATTGGTTAATGTTGTACCAATACTT
  GAAGATAGTTTCAATACAGATATTGGAACGGTTAATATCGCCGTTAGTATAACTGCTTTA
  TTTTCAGGAATGTTTGTAGTAGGAGCAGGTGGTCTTGCTGATAAATATGGCAGAATTAAA
  CTCACGAACATTGGTATTATCTTAAATATATTAGGTTCATTATTAATCATTATTTCAAAT
  ATTCCTTTATTACTTATTATAGGAAGATTAATTCAAGGACTTTCAGCAGCATGTATTATG
  CCTGCAACTTTGTCTATTATTAAGTCATATTACATTGGGAAAGATAGACAACGCGCTTTA
  AGTTATTGGTCAATTGGCTCATGGGGCGGCTCTGGTGTTTGTTCATTTTTTGGAGGTGCA
  GTTGCAACGCTTTTAGGTTGGCGTTGGATTTTCATCCTATCAATTATAATTTCATTAATT
  GCACTGTTTCTTATTAAAGGCACACCTGAAACTAAATCTAAATCGATTTCTCTAAATAAA
  TTTGACATTAAAGGTCTGGTTCTTTTAGTCATTATGCTCCTCAGTTTAAATATTTTAATT
  ACTAAAGGATCAGAATTAGGTGTAACCTCACTTCTTTTTATTACTTTATTAGCTATTGCA
  ATTGGATCTTTTAGTTTATTTATAGTTCTTGAAAAGCGTGCTACAAATCCTTTAATCGAT
  TTTAAATTATTTAAAAATAAAGCTTACACAGGTGCAACAGCTTCAAACTTTTTGTTAAAT
  GGTGTTGCAGGAACATTAATAGTAGCCAACACATTTGTTCAAAGAGGTTTAGGATATTCT
  TCATTGCAAGCAGGAAGTTTATCAATCACTTATTTAGTAATGGTACTAATTATGATTCGT
  GTTGGTGAAAAGTTACTTCAAACACTCGGATGCAAGAAACCAATGTTAATTGGAACAGGA
  GTTCTTATTGTCGGAGAATGTCTCATTTCATTAACTTTCTTGCCAGAAATATTCTATGTC
  ATTTGTTGTATTATAGGTTATTTATTCTTTGGTTTAGGACTAGGGATATATGCTACACCA
  TCAACAGATACAGCAATTGCAAATGCACCGTTAGAAAAAGTAGGCGTTGCTGCAGGTATC
  TATAAAATGGCTTCTGCATTAGGTGGAGCATTTGGCGTCGCATTGAGTGGTGCAGTATAT
  GCAATCGTATCAAATATGACAAACATTTATACAGGTGCAATGATTGCATTATGGTTAAAT
  GCAGGTATGGGAATATTATCATTCGTTATCATTTTGTTACTTGTGCCTAAACAAAACGAT
  ACTCAATTATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1392
  

⊟Protein

⊟General

• locus tag: SAUSA300_1328 [new locus tag: SAUSA300_RS07240 ]
• symbol: SAUSA300_1328
• description: putative drug transporter
• length: 463
• theoretical pI: 9.70893
• theoretical MW: 49292.8
• GRAVY: 0.970194

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.5)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.5)
  and 7 more
  multidrug resistance protein (TIGR00880; HMM-score: 62.6)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 46.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 40.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 31.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 31.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 23.6)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 16.9)
• TheSEED  :
  • putative transporter
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 178.4)
  and 2 more
  TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 7.2)
  PUCC; PUCC protein (PF03209; HMM-score: 5.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.06735
  • TAT(Tat/SPI): 0.003796
  • LIPO(Sec/SPII): 0.018709
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 87161106 NCBI
• RefSeq: YP_494025 NCBI
• UniProt: Q2FH03 UniProt

⊟Protein sequence

• MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications