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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01448
  • pan locus tag?: SAUPAN003890000
  • symbol: SAOUHSC_01448
  • pan gene symbol?: norB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01448
  • symbol: SAOUHSC_01448
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1405096..1406487
  • length: 1392
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGAAAAGCCGTCAAGAGAGGCATTTGAAGGCAATAATAAGTTGTTAATAGGAATTGTT
    CTAAGTGTAATAACGTTTTGGCTATTTGCACAATCATTGGTTAATGTTGTACCAATACTT
    GAAGATAGTTTCAATACAGATATTGGAACGGTTAATATCGCCGTTAGTATAACTGCTTTA
    TTTTCAGGAATGTTTGTAGTAGGAGCAGGTGGTCTTGCTGATAAATATGGCAGAATTAAA
    CTCACGAACATTGGTATTATCTTAAATATATTAGGTTCATTATTAATCATTATTTCAAAT
    ATTCCTTTATTACTTATTATAGGAAGATTAATTCAAGGACTTTCAGCAGCATGTATTATG
    CCTGCAACTTTGTCTATTATTAAGTCATATTACATTGGGAAAGATAGACAACGCGCTTTA
    AGTTATTGGTCAATTGGCTCATGGGGCGGCTCTGGTGTTTGTTCATTTTTTGGAGGTGCA
    GTTGCAACGCTTTTAGGTTGGCGTTGGATTTTCATCCTATCAATTATAATTTCATTAATT
    GCACTGTTTCTTATTAAAGGCACACCTGAAACTAAATCTAAATCGATTTCTCTAAATAAA
    TTTGACATTAAAGGTCTGGTTCTTTTAGTCATTATGCTCCTCAGTTTAAATATTTTAATT
    ACTAAAGGATCAGAATTAGGTGTAACCTCACTTCTTTTTATTACTTTATTAGCTATTGCA
    ATTGGATCTTTTAGTTTATTTATAGTTCTTGAAAAGCGTGCTACAAATCCTTTAATCGAT
    TTTAAATTATTTAAAAATAAAGCTTACACAGGTGCAACAGCTTCAAACTTTTTGTTAAAT
    GGTGTTGCAGGAACATTAATAGTAGCCAACACATTTGTTCAAAGAGGTTTAGGATATTCT
    TCATTGCAAGCAGGAAGTTTATCAATCACTTATTTAGTAATGGTACTAATTATGATTCGT
    GTTGGTGAAAAGTTACTTCAAACACTCGGATGCAAGAAACCAATGTTAATTGGAACAGGA
    GTTCTTATTGTCGGAGAATGTCTCATTTCATTAACTTTCTTGCCAGAAATATTCTATGTC
    ATTTGTTGTATTATAGGTTATTTATTCTTTGGTTTAGGACTAGGGATATATGCTACACCA
    TCAACAGATACAGCAATTGCAAATGCACCGTTAGAAAAAGTAGGCGTTGCTGCAGGTATC
    TATAAAATGGCTTCTGCATTAGGTGGAGCATTTGGCGTCGCATTGAGTGGTGCAGTATAT
    GCAATCGTATCAAATATGACAAACATTTATACAGGTGCAATGATTGCATTATGGTTAAAT
    GCAGGTATGGGAATATTATCATTCGTTATCATTTTGTTACTTGTGCCTAAACAAAACGAC
    ACTCAATTATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1392

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01448
  • symbol: SAOUHSC_01448
  • description: hypothetical protein
  • length: 463
  • theoretical pI: 9.70893
  • theoretical MW: 49292.8
  • GRAVY: 0.970194

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.5)
    Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 131.5)
    and 7 more
    multidrug resistance protein (TIGR00880; HMM-score: 62.6)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 46.1)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 40.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 31.8)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 31.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 23.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 16.9)
  • TheSEED  :
    • putative transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 178.4)
    and 2 more
    TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 7.2)
    PUCC; PUCC protein (PF03209; HMM-score: 5.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.06735
    • TAT(Tat/SPI): 0.003796
    • LIPO(Sec/SPII): 0.018709
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Q C Truong-Bolduc, P M Dunman, J Strahilevitz, S J Projan, D C Hooper
MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus.
J Bacteriol: 2005, 187(7);2395-405
[PubMed:15774883] [WorldCat.org] [DOI] (P p)Que Chi Truong-Bolduc, David C Hooper
The transcriptional regulators NorG and MgrA modulate resistance to both quinolones and beta-lactams in Staphylococcus aureus.
J Bacteriol: 2007, 189(8);2996-3005
[PubMed:17277059] [WorldCat.org] [DOI] (P p)

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