PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1318 [new locus tag: SAUSA300_RS07180 ]
• pan locus tag^?: SAUPAN003857000
• symbol: SAUSA300_1318
• pan gene symbol^?: fakB2
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1318 [new locus tag: SAUSA300_RS07180 ]
• symbol: SAUSA300_1318
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1449876..1450715
• length: 840
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914245 NCBI
• RefSeq: YP_494015 NCBI
• BioCyc: see SAUSA300_RS07180
• MicrobesOnline: 1292833 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAAAACAGATTATAGTAACAGACTCAACATCCGATTTATCTAAAGAATACTTAGAA
  GCAAACAACATTCATGTAATTCCTTTAAGTTTAACTATTGAAGGAGCTTCATACGTTGAC
  CAAGTAGATATTACATCAGAAGAATTTATTAATCATATTGAAAATGATGAAGATGTAAAG
  ACAAGTCAGCCAGCCATAGGTGAATTTATATCTGCTTATGAAGAACTAGGAAAAGATGGC
  TCTGAAATCATAAGTATTCATCTTTCTTCAGGATTAAGTGGTACATATAACACTGCTTAC
  CAAGCAAGTCAAATGGTAGATGCTAATGTAACTGTTATTGATTCAAAATCTATTTCTTTT
  GGTTTAGGGTATCAAATACAACACCTAGTAGAGCTTGTAAAAGAAGGTGTCTCAACTTCT
  GAAATAGTTAAAAAGTTAAATCATTTAAGAGAAAACATTAAATTATTTGTAGTTATAGGG
  CAATTGAATCAATTAATTAAAGGTGGCAGAATTAGTAAAACAAAAGGTTTGATTGGTAAT
  CTTATGAAAATTAAACCAATTGGTACACTAGATGATGGTCGCTTAGAGCTTGTGCACAAT
  GCGAGAACTCAAAATTCTAGTATCCAATACTTGAAAAAGGAAATTGCTGAATTTATAGGA
  GATCATGAAATCAAATCCATTGGTGTCGCACATGCTAACGTCATTGAATATGTTGATAAA
  TTGAAGAAAGTTTTTAATGAAGCTTTTCATGTGAATAATTACGATATAAATGTAACTACA
  CCAGTTATTTCTGCACATACTGGTCAAGGTGCGATTGGCCTCGTAGTCCTTAAGAAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1318 [new locus tag: SAUSA300_RS07180 ]
• symbol: SAUSA300_1318
• description: hypothetical protein
• length: 279
• theoretical pI: 5.7835
• theoretical MW: 30644.6
• GRAVY: -0.0921147

⊟Function

• TIGRFAM:
  Unknown function General EDD domain protein, DegV family (TIGR00762; HMM-score: 330.9)
• TheSEED  :
  • Hypothetical protein DUF194, DegV family
• PFAM:
  EDD (CL0245) DegV; Uncharacterised protein, DegV family COG1307 (PF02645; HMM-score: 286.8)
  and 1 more
  Dak1_2; Dihydroxyacetone kinase family (PF13684; HMM-score: 37)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003441
  • TAT(Tat/SPI): 0.000229
  • LIPO(Sec/SPII): 0.000313
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162066 NCBI
• RefSeq: YP_494015 NCBI
• UniProt: A0A0H2XI23 UniProt

⊟Protein sequence

• MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDITSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQASQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIGQLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEFIGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVVLKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1318 < folA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Joshua B Parsons, Tyler C Broussard, Jeffrey L Bose, Jason W Rosch, Pamela Jackson, Chitra Subramanian, Charles O Rock
Identification of a two-component fatty acid kinase responsible for host fatty acid incorporation by Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2014, 111(29);10532-7
[PubMed:25002480] [WorldCat.org] [DOI] (I p)