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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0899 [new locus tag: SAUSA300_RS04845 ]
  • pan locus tag?: SAUPAN003168000
  • symbol: SAUSA300_0899
  • pan gene symbol?: trfA
  • synonym:
  • product: adaptor protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0899 [new locus tag: SAUSA300_RS04845 ]
  • symbol: SAUSA300_0899
  • product: adaptor protein
  • replicon: chromosome
  • strand: +
  • coordinates: 987736..988455
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGAATAGAACGAGTAGATGATACAACTGTAAAATTGTTTATAACATATAGCGATATC
    GAGGCCCGTGGATTTAGTCGTGAAGATTTATGGACAAATCGCAAACGTGGCGAAGAATTC
    TTTTGGTCAATGATGGATGAAATTAACGAAGAAGAAGATTTTGTTGTAGAAGGTCCATTA
    TGGATTCAAGTACATGCCTTTGAAAAAGGTGTCGAAGTCACAATTTCTAAATCTAAAAAT
    GAAGATATGATGAATATGTCTGATGATGATGCAACTGATCAATTTGATGAACAAGTTCAA
    GAATTGTTAGCTCAAACATTAGAAGGTGAAGATCAATTAGAAGAATTATTCGAGCAACGA
    ACAAAAGAAAAAGAAGCTCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACA
    AGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGC
    AATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTAT
    GCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTG
    CTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTAAAATA
    ATTATGAGTCATAACGTAACAGCTCAAGTTCGACGTTATTTTCCAGAGACAACTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0899 [new locus tag: SAUSA300_RS04845 ]
  • symbol: SAUSA300_0899
  • description: adaptor protein
  • length: 239
  • theoretical pI: 4.12478
  • theoretical MW: 28325
  • GRAVY: -0.747699

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Negative regulator of genetic competence MecA
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Negative regulator of genetic competence MecA
  • PFAM:
    no clan defined MecA; Negative regulator of genetic competence (MecA) (PF05389; HMM-score: 183)
    and 3 more
    NACHT_N (CL0864) Goodbye; fungal STAND N-terminal Goodbye domain (PF17109; HMM-score: 14.9)
    HTH (CL0123) Rep3_N; Initiator Replication protein, WH1 (PF01051; HMM-score: 11.5)
    no clan defined Rsc14; RSC complex, Rsc14/Ldb7 subunit (PF08586; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.975
    • Cytoplasmic Membrane Score: 0.0228
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0008
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003504
    • TAT(Tat/SPI): 0.000246
    • LIPO(Sec/SPII): 0.000504
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSMMDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQELLAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHSNPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYAKIIMSHNVTAQVRRYFPETTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

TrfA binds to the ClpCP protease and increases its degradation of SsrA-tagged proteins (generated by stalled ribosomes) and degradation of class II "addiction module" antitoxins. TrfA itself is proteolyzed by ClpCP making its CplCP specificity redirecting "adaptor" activity highly dependent on the trfA transcription rate.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Niles P Donegan, Jonathan S Marvin, Ambrose L Cheung
    Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus.
    J Bacteriol: 2014, 196(23);4140-51
    [PubMed:25225270] [WorldCat.org] [DOI] (I p)
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