From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0868 [new locus tag: NWMN_RS04875 ]
  • pan locus tag?: SAUPAN003168000
  • symbol: NWMN_0868
  • pan gene symbol?: trfA
  • synonym:
  • product: adaptor protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0868 [new locus tag: NWMN_RS04875 ]
  • symbol: NWMN_0868
  • product: adaptor protein
  • replicon: chromosome
  • strand: +
  • coordinates: 964382..965101
  • length: 720
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAGAATAGAACGAGTAGATGATACAACTGTAAAATTGTTTATAACATATAGCGATATC
    GAGGCCCGTGGATTTAGTCGTGAAGATTTATGGACAAATCGCAAACGTGGCGAAGAATTC
    TTTTGGTCAATGATGGATGAAATTAACGAAGAAGAAGATTTTGTTGTAGAAGGTCCATTA
    TGGATTCAAGTACATGCCTTTGAAAAAGGTGTCGAAGTCACAATTTCTAAATCTAAAAAT
    GAAGATATGATGAATATGTCTGATGATGATGCAACTGATCAATTTGATGAACAAGTTCAA
    GAATTGTTAGCTCAAACATTAGAAGGTGAAGATCAATTAGAAGAATTATTCGAGCAACGA
    ACAAAAGAAAAAGAAGCTCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACA
    AGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGC
    AATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTAT
    GCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTG
    CTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTAAAATA
    ATTATGAGTCATAACGTAACAGCTCAAGTTCGACGTTATTTTCCAGAGACAACTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0868 [new locus tag: NWMN_RS04875 ]
  • symbol: NWMN_0868
  • description: adaptor protein
  • length: 239
  • theoretical pI: 4.12478
  • theoretical MW: 28325
  • GRAVY: -0.747699

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Negative regulator of genetic competence MecA
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Negative regulator of genetic competence MecA
  • PFAM:
    no clan defined MecA; Negative regulator of genetic competence (MecA) (PF05389; HMM-score: 183)
    and 3 more
    NACHT_N (CL0864) Goodbye; fungal STAND N-terminal Goodbye domain (PF17109; HMM-score: 14.9)
    HTH (CL0123) Rep3_N; Initiator Replication protein, WH1 (PF01051; HMM-score: 11.5)
    no clan defined Rsc14; RSC complex, Rsc14/Ldb7 subunit (PF08586; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.975
    • Cytoplasmic Membrane Score: 0.0228
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0008
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003504
    • TAT(Tat/SPI): 0.000246
    • LIPO(Sec/SPII): 0.000504
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSMMDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQELLAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHSNPITTEFEDLLYMVDGTYYYAVYFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYAKIIMSHNVTAQVRRYFPETTE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_0868&oldid=58370"