PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0316 [new locus tag: SAUSA300_RS01685 ]
• pan locus tag^?: SAUPAN001240000
• symbol: SAUSA300_0316
• pan gene symbol^?: nanK
• synonym:
• product: ROK family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0316 [new locus tag: SAUSA300_RS01685 ]
• symbol: SAUSA300_0316
• product: ROK family protein
• replicon: chromosome
• strand: +
• coordinates: 367811..368671
• length: 861
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914591 NCBI
• RefSeq: YP_493030 NCBI
• BioCyc: see SAUSA300_RS01685
• MicrobesOnline: 1291831 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGTATTACATCGCAATCGATATTGGAGGCACTCAAATTAAATCGGCAGTTATTGATAAG
  CAATTGAATATGTTTGACTATCAACAAATATCAACGCCGGACAACAAAAGTGAGCTTATT
  ACTGACAAAGTATATGAGATTGTAACAGGATATATGAAGCAATATCAGTTGATCCAACCT
  GTCATAGGTATTTCATCAGCAGGCGTTGTTGATGAACAAAAAGGCGAAATTGTATACGCA
  GGGCCAACCATTCCGAATTATAAAGGTACTAATTTTAAGCGATTATTAAAATCACTGTCT
  CCTTATGTCAAAGTAAAAAATGATGTAAACGCTGCATTACTAGGCGAATTGAAATTACAT
  CAATATCAAGCAGAACGGATCTTTTGTATGACGCTTGGTACAGGCATTGGGGGTGCGTAC
  AAGAATAATCAAGGTCATATTGATAATGGTGAGCTTCATAAGGCAAATGAAGTTGGGTAT
  TTATTGTATCGTCCAACTGAAAATACAACGTTTGAGCAACGTGCTGCAACGAGTGCATTG
  AAAAAGCGCATGATTGCCGGAGGATTTACGAGAAGCACACATGTGCCAGTATTGTTTGAA
  GCAGCTGAAGAAGGTGATGATATTGCAAAACAAATATTGAATGAGTGGGCAGAAGATGTA
  GCAGAAGGGATTGCCCAAATACAGGTCATGTATGATCCAGGGCTTATATTAATTGGGGGC
  GGTATATCTGAACAAGGAGATAATCTCATTAAATATATCGAGCCGAAAGTTGCACACTAT
  TTACCAAAAGACTATGTTTATGCACCAATACAAACGACTAAGAGTAAAAATGATGCAGCA
  TTATATGGCTGTTTGCAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0316 [new locus tag: SAUSA300_RS01685 ]
• symbol: SAUSA300_0316
• description: ROK family protein
• length: 286
• theoretical pI: 5.69776
• theoretical MW: 31682.9
• GRAVY: -0.262238

⊟Function

• TIGRFAM:
  Unknown function General ROK family protein (putative glucokinase) (TIGR00744; HMM-score: 112.5)
• TheSEED  :
  • N-acetylmannosamine kinase (EC 2.7.1.60)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylmannosamine kinase (EC 2.7.1.60)
• PFAM:
  Actin_ATPase (CL0108) ROK; ROK family (PF00480; HMM-score: 127.8)
  and 3 more
  BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 20.4)
  Pan_kinase; Type III pantothenate kinase (PF03309; HMM-score: 13.6)
  FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004312
  • TAT(Tat/SPI): 0.000111
  • LIPO(Sec/SPII): 0.000616
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161931 NCBI
• RefSeq: YP_493030 NCBI
• UniProt: A0A0H2XJN5 UniProt

⊟Protein sequence

• MYYIAIDIGGTQIKSAVIDKQLNMFDYQQISTPDNKSELITDKVYEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPYVKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGYLLYRPTENTTFEQRAATSALKKRMIAGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAEDVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSKNDAALYGCLQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulators: NanR* (repression) regulon, CcpA regulon

  NanR*	(TF)	important in Sialic acid utilization, N-acetylglucosamine utilization; RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Michael E Olson, Jessica M King, Timothy L Yahr, Alexander R Horswill
Sialic acid catabolism in Staphylococcus aureus.
J Bacteriol: 2013, 195(8);1779-88
[PubMed:23396916] [WorldCat.org] [DOI] (I p)