Jump to navigation
Jump to search
⊟Summary[edit | edit source]
- pan ID?: SAUPAN005495000
- symbol?: mtlA
- synonym:
- description?: PTS mannitol transporter subunit IICB
- PTS mannitol transporter subunit IICB
- PTS system, mannitol specific IIBC component
- PTS system, mannitol-specific IIBC components
- PTS system mannitol-specific protein
- PTS system mannitol specific transporter subunit IIBC
- PTS system mannitol specific IIBC component
- mannitol specific IIBC component of PTS system
- Mannitol-specific phosphotransferase enzyme IIB component
- PTS family fructose/mannitol (fru) porter component IIBC
- PTS family mannitol porter, EIICB component
- PTS system, mannitol-specific IIB component / PTS system, mannitol-specific IIC component
- pts system mannitol-specific eiicba component (eiicba-mtl) (eii-mtl)
- PTS system mannitol-specific EIICB component
- PTS system mannitol-specific transporter subunit IIC
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 5651125..5652675
- synteny block?: BlockID0042770
- occurrence?: in 100% of 34 strains
mtlA : mannitol-specific phosphotransferase system IIBC MtlA [1]
In addition to its role as a carbon source, mannitol is a compatible solute that can impact the osmotic balance of the staphylococcal cell. Therefore, mannitol transportation by phosphotransferase systems can be critical to staphylococcal viability. Phosphotransferase systems couple sugar transport to sugar phosphorylation which traps the substrate in the cytoplasm. All phosphotransferase systems share the common transfer of activated phosphate from phosphoenolpyruvate to Complex EI and then on to the soluble phosphocarrier protein HPr. HPr can then transfer its phosphate to sugar-specific complex EIIA (acceptor-activator) and EIIBC (kinase-transporter) subunits. Mannitol-specific IIA and IIBC subunits are encoded by mtlF and mtlA, respectively. MtlA serves an additional role by phosphorylating the mannitol transport transcriptional activator MtlR at HIS-231 or HIS-290 resulting in decreased DNA binding and reduced mannitol operon activation. However, when mannitol is present, MtlA preferentially phosphorylates mannitol and dephosphorylates MtlR, resulting in transcriptional activation. Please note, some annotations describe the mannitol transport operon with MtlF as the IIBC component and MtlA as the IIA component. For consistency with the majority of firmicute annotations, we will here annotate the IIBC component as "MtlA" and the IIA component as "MtlF".
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2095 (mtlF)
08BA02176:
C248_2184 (mtlA)
11819-97:
MS7_2171 (mtlA)
6850:
RSAU_001992 (mtlF)
71193:
ST398NM01_2210
ECT-R 2:
ECTR2_2009 (mtlA)
ED133:
SAOV_2195
ED98:
SAAV_2210
HO 5096 0412:
SAEMRSA15_20620 (mtlA)
JH1:
SaurJH1_2226
JH9:
SaurJH9_2188
JKD6008:
SAA6008_02192 (mtlA)
JKD6159:
SAA6159_02065 (mtlA)
JSNZ:
JSNZ_002116
LGA251:
SARLGA251_19520 (mtlA)
M013:
M013TW_2114
MRSA252:
SAR2244 (mtlA)
MSHR1132:
SAMSHR1132_19880
MSSA476:
SAS2057
Mu3:
SAHV_2140 (mtlF)
Mu50:
SAV2156 (mtlF)
MW2:
MW2082 (mtlF)
RF122:
SAB2036 (mtlA)
ST398:
SAPIG2210
T0131:
SAT0131_02319
TCH60:
HMPREF0772_11037 (mtlA)
TW20:
SATW20_22910 (mtlA)
USA300_TCH1516:
USA300HOU_2146 (mtlF)
VC40:
SAVC_09645
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
N315 MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
NCTC8325 MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
Newman MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
USA300_FPR3757 MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPM
************************************************************
COL ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
N315 ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
NCTC8325 ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
Newman ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
USA300_FPR3757 ITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKT
************************************************************
COL DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
N315 DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
NCTC8325 DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
Newman DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
USA300_FPR3757 DQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLL
************************************************************
COL PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
N315 PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
NCTC8325 PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
Newman PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
USA300_FPR3757 PLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIF
************************************************************
COL GKGTAKTTSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
N315 GKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
NCTC8325 GKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
Newman GKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
USA300_FPR3757 GKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSP
******:*****************************************************
COL ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
N315 ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
NCTC8325 ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
Newman ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
USA300_FPR3757 ASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENT
************************************************************
COL KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
N315 KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
NCTC8325 KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
Newman KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
USA300_FPR3757 KGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINH
************************************************************
COL VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
N315 VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
NCTC8325 VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
Newman VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
USA300_FPR3757 VIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIK
************************************************************
COL QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
N315 QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
NCTC8325 QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
Newman QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
USA300_FPR3757 QTPNAIHISVDNFLNSPRYEELLNNLKKDDQA
********************************
- ↑ S A Henstra, M Tuinhof, R H Duurkens, G T Robillard
The Bacillus stearothermophilus mannitol regulator, MtlR, of the phosphotransferase system. A DNA-binding protein, regulated by HPr and iicbmtl-dependent phosphorylation.
J Biol Chem: 1999, 274(8);4754-63
[PubMed:9988713] [WorldCat.org] [DOI] (P p)Thanh Nguyen, Truc Kim, Hai Minh Ta, Won Sik Yeo, Jongkeun Choi, Pushpak Mizar, Seung Seo Lee, Taeok Bae, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
Targeting Mannitol Metabolism as an Alternative Antimicrobial Strategy Based on the Structure-Function Study of Mannitol-1-Phosphate Dehydrogenase in Staphylococcus aureus.
mBio: 2019, 10(4);
[PubMed:31289190] [WorldCat.org] [DOI] (I e)Nicholas P Vitko, Melinda R Grosser, Dal Khatri, Thurlow R Lance, Anthony R Richardson
Expanded Glucose Import Capability Affords Staphylococcus aureus Optimized Glycolytic Flux during Infection.
mBio: 2016, 7(3);
[PubMed:27329749] [WorldCat.org] [DOI] (I e)