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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1960 [new locus tag: SA_RS11250 ]
  • pan locus tag?: SAUPAN005495000
  • symbol: mtlF
  • pan gene symbol?: mtlA
  • synonym:
  • product: PTS system mannitol specific transporter subunit IIBC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1960 [new locus tag: SA_RS11250 ]
  • symbol: mtlF
  • product: PTS system mannitol specific transporter subunit IIBC
  • replicon: chromosome
  • strand: +
  • coordinates: 2212569..2214107
  • length: 1539
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCGCAAACTGAAGAGAAAAAAGGAATTGGTCGTCGCGTTCAAGCATTCGGATCATTT
    TTGAGTAGTATGATTATGCCAAACATTGGTGCATTCATCGCTTGGGGTTTCATCGCGGCC
    ATTTTTATAGATAATGGATGGTTACCTAACAAAGATTTAGCTACTTTAGCAGGACCAATG
    ATTACTTATTTAATCCCATTATTGATCGCATTTAGTGGTGGTCGTTTAATTTATGATTTA
    CGTGGTGGTATCATCGCAGCAACAGCAACTATGGGGGTCATCGTTGCATTACCTGATACA
    CCAATGTTACTTGGTGCAATGATTATGGGTCCACTTGTTGGTTGGTTAATGAAGAAGACC
    GACCAATTGATTCAACCTAGAACGCCACAAGGTTTTGAAATGTTATTTAATAACTTTTCT
    GCTGGTATTTTAGGATTTATCATGACGATTGCAGGATTTAAAATTTTAGCACCACTTATG
    AAATTTATTATGCATATTTTATCAGTGGCAGTTGAAGCATTAGTACATGCACACCTACTT
    CCACTTGTAAGTATTTTAGTAGAGCCTGCAAAAATTGTATTTTTAAACAATGCGATTAAC
    CATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTA
    TACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTT
    GGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGGT
    ATTCATGAAATTTATTTCCCATATGTATTAATGCGTCCTTTATTATTTATCGCTGTTATT
    TTAGGTGGAATGACTGGTGTTGCAACTTATCAAGCAACAGGATTTGGATTTAAAAGTCCC
    GCGTCACCAGGTTCATTTATAGTTTACTGTTTAAATGCACCTAGAGGTGAATTCTTGCAC
    ATGTTGCTCGGTGTCTTCCTTGCTGCACTTGTATCATTCGTTGTAGCTGCTTTAATTATG
    AAGTTCACTAGAGAACCAAAGCAGGATTTAGAAGCTGCGACAGCTCAAATGGAAAATACT
    AAAGGGAAAAAATCAAGCGTTGCTTCTAAGTTAGTATCTTCTGATAAAAATGTTAATACA
    GAAGAAAATGCTAGTGGTAATGTTAGTGAAACATCTTCATCAGATGATGATCCTGAAGCG
    CTATTGGATAATTACAACACTGAAGATGTTGATGCACACAATTACAATAATATAAATCAT
    GTTATTTTTGCCTGCGATGCGGGTATGGGTTCTAGTGCAATGGGTGCAAGCATGTTACGT
    AATAAATTTAAAAAGGCGGGCATTAATGATATTACAGTTACAAATACTGCGATTAATCAA
    TTGCCAAAAGATGCTCAATTAGTTATCACTCAGAAAAAACTAACTGATCGTGCTATTAAA
    CAAACACCAAATGCCATCCATATTTCAGTGGATAATTTCCTTAATTCACCAAGATATGAA
    GAACTTTTAAATAATCTAAAAAAAGATGATCAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1539

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1960 [new locus tag: SA_RS11250 ]
  • symbol: MtlF
  • description: PTS system mannitol specific transporter subunit IIBC
  • length: 512
  • theoretical pI: 7.76606
  • theoretical MW: 55064.9
  • GRAVY: 0.299219

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.197?  ExPASy
    Protein-N(pi)-phosphohistidine--D-mannitol phosphotransferase [Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2)
  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 589)
    Signal transduction Signal transduction PTS PTS system, mannitol-specific IIC component (TIGR00851; EC 2.7.1.69; HMM-score: 589)
    and 2 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 14.9)
  • TheSEED  :
    • PTS system, mannitol-specific IIB component (EC 2.7.1.197)
    • PTS system, mannitol-specific IIC component
    Carbohydrates Sugar alcohols Mannitol Utilization  PTS system, mannitol-specific IIB component (EC 2.7.1.69)
    and 1 more
    Carbohydrates Sugar alcohols Mannitol Utilization  PTS system, mannitol-specific IIC component (EC 2.7.1.69)
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 60.2)
    and 3 more
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 44)
    Rhodanese; Rhodanese-like domain (PF00581; HMM-score: 16.4)
    no clan defined Ctr; Ctr copper transporter family (PF04145; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009005
    • TAT(Tat/SPI): 0.001102
    • LIPO(Sec/SPII): 0.003247
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFIDNGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPMLLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLMKFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQSILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLFIAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFVVAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETSSSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGINDITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKKDDQA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: MtlR* (activation) regulon
    MtlR*(TF)important in Mannitol utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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