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⊟Summary[edit | edit source]
- pan ID?: SAUPAN005408000
- symbol?: prmC
- synonym:
- description?: protein-(glutamine-N5) methyltransferase, release factor-specific
- protein-(glutamine-N5) methyltransferase, release factor-specific
- HemK family modification methylase
- peptide chain release factor N(5)-glutamine methyltransferase
- modification methylase HemK family protein
- N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase, putative
- HemK family methyltransferase
- methylase of polypeptide chain release factor
- Methylase of polypeptide chain release factors
- N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase
- peptide release factor-glutamine N5-methyltransferase
- protein-(glutamine-N5) methyltransferase
- protoporphyrinogen oxidase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5567977..5568729
- synteny block?: BlockID0042040
- occurrence?: in 100% of 34 strains
prmC (hemK) : peptide chain release factor glutamine N(5)-methyltransferase [1]
Ribosomal peptide release has not been extensively characterized in firmicutes and functions are therefore extrapolated from foundational studies in E. coli and pseudomonas. Peptide chain release factors require a post-translational modification, specifically glutamine methylation, to efficiently release new peptides from ribosomes at stop codons. PrmC recognizes the conserved "GGQ" motif of Release Factor 1 or Release Factor 2 and transfers a methyl group from S-adenosylmethionine to the Release Factor's glutamine.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2057
08BA02176:
C248_2131
11819-97:
MS7_2133 (prmC)
6850:
RSAU_001955
71193:
ST398NM01_2159
ECT-R 2:
ECTR2_1973 (hemK)
ED133:
SAOV_2158c
ED98:
SAAV_2172
HO 5096 0412:
SAEMRSA15_20250
JH1:
SaurJH1_2191
JH9:
SaurJH9_2153
JKD6008:
SAA6008_02155
JKD6159:
SAA6159_02031
JSNZ:
JSNZ_002078 (prmC)
LGA251:
SARLGA251_19180
M013:
M013TW_2076
MRSA252:
SAR2205
MSHR1132:
SAMSHR1132_19400
MSSA476:
SAS2020
Mu3:
SAHV_2101
Mu50:
SAV2117
MW2:
MW2041
RF122:
SAB2001c
ST398:
SAPIG2159 (hemK)
T0131:
SAT0131_02280
TCH60:
HMPREF0772_11074 (prmC)
TW20:
SATW20_22550
USA300_TCH1516:
USA300HOU_2105
VC40:
SAVC_09460
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
N315 MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
NCTC8325 ----------------------------MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
Newman MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
USA300_FPR3757 ----------------------------MLDVFQWTRTDFVVHMHDDMPKAMIMKFDLAL
********************************
COL QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
N315 QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
NCTC8325 QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
Newman QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
USA300_FPR3757 QRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLHFLQQLEDDATIVDIGTGS
************************************************************
COL GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
N315 GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLIKEGIKLNG
NCTC8325 GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
Newman GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
USA300_FPR3757 GVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQIQFLTGDALKPLINEGIKLNG
****************************************************:*******
COL LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
N315 LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIGDLPHVMEKGSPVVFEI
NCTC8325 LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
Newman LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
USA300_FPR3757 LISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAIYESIIEDLPHVMEKGSPVVFEI
******************************************* ****************
COL GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
N315 GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
NCTC8325 GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
Newman GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
USA300_FPR3757 GYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSFKW
**************************************
- ↑ Heidi L Schubert, John D Phillips, Christopher P Hill
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.
Biochemistry: 2003, 42(19);5592-9
[PubMed:12741815] [WorldCat.org] [DOI] (P p)Marc Graille, Valérie Heurgué-Hamard, Stéphanie Champ, Liliana Mora, Nathalie Scrima, Nathalie Ulryck, Herman van Tilbeurgh, Richard H Buckingham
Molecular basis for bacterial class I release factor methylation by PrmC.
Mol Cell: 2005, 20(6);917-27
[PubMed:16364916] [WorldCat.org] [DOI] (P p)