Jump to navigation
Jump to search
⊟Summary[edit | edit source]
- pan ID?: SAUPAN005405000
- symbol?: —
- synonym:
- description?: TIGR01440 family protein
- TIGR01440 family protein
- hypothetical protein with DUF436
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5565764..5566162
- synteny block?: BlockID0042010
- occurrence?: in 100% of 34 strains
ywlG (efpG) : multifunctional HPF-sequestering glutamate dehydrogenase YwlG [1]
• A crystal structure is available : 7Z06
YwlG is a classic example of staphylococcal enzymes moonlighting to perform multiple functions. At its core, YwlG is an allosterically-regulated glutamate dehydrogenase. In this context, it likely provides an amino group for the post-translational 5-aminopentanol modification of Elongation Factor P. YwlG physically interacts with Hibernation-Promoting Factor with the mutual sequestration inhibiting YwlG's glutamate dehydrogenase activity and HPF's 100S hibernation ribosome assembly activity. A YwlG knockout has pronounced colonization deficits in both pneumonia and osteomyelitis infection models making it a bona fide virulence factor.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2054
08BA02176:
C248_2128
11819-97:
MS7_2130
6850:
RSAU_001952
71193:
ST398NM01_2156
ECT-R 2:
ECTR2_1970
ED133:
SAOV_2155c
ED98:
SAAV_2169
HO 5096 0412:
SAEMRSA15_20220
JH1:
SaurJH1_2188
JH9:
SaurJH9_2150
JKD6008:
SAA6008_02152
JKD6159:
SAA6159_02028
JSNZ:
JSNZ_002075
LGA251:
SARLGA251_19150
M013:
M013TW_2073
MRSA252:
SAR2202
MSHR1132:
SAMSHR1132_19370
MSSA476:
SAS2017
Mu3:
SAHV_2098
Mu50:
SAV2114
MW2:
MW2038
RF122:
SAB1998c
ST398:
SAPIG2156
T0131:
SAT0131_02276
TCH60:
HMPREF0772_11077 (ywlG)
TW20:
SATW20_22520
USA300_TCH1516:
USA300HOU_2104
VC40:
SAVC_09445
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSMEVAETIFNALDVVSKET
N315 MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSMEVAETIFNALDVVSKET
NCTC8325 MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSMEVAETIFNALDVVSKET
Newman MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSMEVAETIFNALDVVSKET
USA300_FPR3757 MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSMEVAETIFNALDVVSKET
************************************************************
COL GVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGSLATYAFQHMKDPIVVEHI
N315 GVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGSLATYAFQHMKDPIVVEHI
NCTC8325 GVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGSLATYAFQHMKDPIVVEHI
Newman GVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGSLATYAFQHMKDPIVVEHI
USA300_FPR3757 GVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGSLATYAFQHMKDPIVVEHI
************************************************************
COL TVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIATSRPKKIGGERAKYQ
N315 TVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIATSRPKKIGGERAKYK
NCTC8325 TVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIATSRPKKIGGERAKYQ
Newman TVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIATSRPKKIGGERAKYQ
USA300_FPR3757 TVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIATSRPKKIGGERAKYQ
*****************************************************:
- ↑ Aimee D Wilde, Daniel J Snyder, Nicole E Putnam, Michael D Valentino, Neal D Hammer, Zachery R Lonergan, Scott A Hinger, Esar E Aysanoa, Catlyn Blanchard, Paul M Dunman, Gregory A Wasserman, John Chen, Bo Shopsin, Michael S Gilmore, Eric P Skaar, James E Cassat
Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection.
PLoS Pathog: 2015, 11(12);e1005341
[PubMed:26684646] [WorldCat.org] [DOI] (I e)Anne Witzky, Katherine R Hummels, Rodney Tollerson, Andrei Rajkovic, Lisa A Jones, Daniel B Kearns, Michael Ibba
EF-P Posttranslational Modification Has Variable Impact on Polyproline Translation in Bacillus subtilis.
mBio: 2018, 9(2);
[PubMed:29615499] [WorldCat.org] [DOI] (I e)Gyu-Lee Kim, Thomas A Hooven, Javiera Norambuena, Barry Li, Jeffrey M Boyd, Jason H Yang, Dane Parker
Growth and Stress Tolerance Comprise Independent Metabolic Strategies Critical for Staphylococcus aureus Infection.
mBio: 2021, 12(3);e0081421
[PubMed:34101490] [WorldCat.org] [DOI] (I p)David Ranava, Christopher M Scheidler, Martin Pfanzelt, Michaela Fiedler, Stephan A Sieber, Sabine Schneider, Mee-Ngan F Yap
Bidirectional sequestration between a bacterial hibernation factor and a glutamate metabolizing protein.
Proc Natl Acad Sci U S A: 2022, 119(39);e2207257119
[PubMed:36122228] [WorldCat.org] [DOI] (I p)