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⊟Summary[edit | edit source]
- pan ID?: SAUPAN005402000
- symbol?: mnaA
- synonym:
- description?: UDP-GlcNAc 2-epimerase
- UDP-GlcNAc 2-epimerase
- UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
- non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase
- UDP-N-acetylglucosamine 2-epimerase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 5562687..5563818
- synteny block?: BlockID0041980
- occurrence?: in 100% of 34 strains
mnaA : UDP-N-acetylglucosamine 2-epimerase [1]
• A crystal structure is available : 5ENZ
Undecaprenol-pyrophosphoryl-N-acetylglucosamine 2-epimerase interconverts UDP-GlcNAc and UDP-ManNAc. This activity is essential to provide substrates for wall teichoic acid biosynthesis and mature biofilm production. Deactivation of MnaA results in complete loss of WTA and beta lactam hypersusceptibility in most staphylococci. However, S. aureus contains a second UDP-GlcNAc 2-epimerase (CapP) which provides a redundant function in capsular biosynthesis. Therefore, a MnaA CapP double-mutant is required for complete loss of UDP-ManNAc biosynthesis in S. aureus.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2051 (mnaA)
08BA02176:
C248_2125 (mnaA)
11819-97:
MS7_2127
6850:
RSAU_001949 (mnaA)
71193:
ST398NM01_2153
ECT-R 2:
ECTR2_1967
ED133:
SAOV_2152c
ED98:
SAAV_2166
HO 5096 0412:
SAEMRSA15_20190 (mnaA)
JH1:
SaurJH1_2185
JH9:
SaurJH9_2147
JKD6008:
SAA6008_02149 (mnaA)
JKD6159:
SAA6159_02025 (mnaA)
JSNZ:
JSNZ_002072 (wecB)
LGA251:
SARLGA251_19120 (mnaA)
M013:
M013TW_2070
MRSA252:
SAR2199 (mnaA)
MSHR1132:
SAMSHR1132_19340
MSSA476:
SAS2014
Mu3:
SAHV_2095 (mnaA)
Mu50:
SAV2111 (mnaA)
MW2:
MW2035 (mnaA)
RF122:
SAB1995c (mnaA)
ST398:
SAPIG2153
T0131:
SAT0131_02273
TCH60:
HMPREF0772_11080 (mnaA)
TW20:
SATW20_22490 (mnaA)
USA300_TCH1516:
USA300HOU_2101 (mnaA)
VC40:
SAVC_09430
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL -MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDL
N315 MMKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDL
NCTC8325 -MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDL
Newman MMKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDL
USA300_FPR3757 -MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDL
***********************************************************
COL NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA
N315 NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA
NCTC8325 NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA
Newman NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA
USA300_FPR3757 NIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEA
************************************************************
COL GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK
N315 GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK
NCTC8325 GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK
Newman GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK
USA300_FPR3757 GLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMK
************************************************************
COL YTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHK
N315 YTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHK
NCTC8325 YTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHK
Newman YTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHK
USA300_FPR3757 YTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHK
************************************************************
COL NPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVL
N315 NPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVL
NCTC8325 NPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVL
Newman NPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVL
USA300_FPR3757 NPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVL
************************************************************
COL RSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVN
N315 RSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVN
NCTC8325 RSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVN
Newman RSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVN
USA300_FPR3757 RSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVN
************************************************************
COL HIKYYLNLITEKPSDF
N315 HIKYYLNLITEKPSDF
NCTC8325 HIKYYLNLITEKPSDF
Newman HIKYYLNLITEKPSDF
USA300_FPR3757 HIKYYLNLITEKPSDF
****************
- ↑ K B Kiser, N Bhasin, L Deng, J C Lee
Staphylococcus aureus cap5P encodes a UDP-N-acetylglucosamine 2-epimerase with functional redundancy.
J Bacteriol: 1999, 181(16);4818-24
[PubMed:10438750] [WorldCat.org] [DOI] (P p)Paul A Mann, Anna Müller, Kerstin A Wolff, Thierry Fischmann, Hao Wang, Patricia Reed, Yan Hou, Wenjin Li, Christa E Müller, Jianying Xiao, Nicholas Murgolo, Xinwei Sher, Todd Mayhood, Payal R Sheth, Asra Mirza, Marc Labroli, Li Xiao, Mark McCoy, Charles J Gill, Mariana G Pinho, Tanja Schneider, Terry Roemer
Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets.
PLoS Pathog: 2016, 12(5);e1005585
[PubMed:27144276] [WorldCat.org] [DOI] (I e)Erika Chang de Azevedo, Alessandro S Nascimento
Energy landscape of the domain movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase.
J Struct Biol: 2019, 207(2);158-168
[PubMed:31088716] [WorldCat.org] [DOI] (I p)