PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2065 [new locus tag: SAUSA300_RS11375 ]
• pan locus tag^?: SAUPAN005402000
• symbol: SAUSA300_2065
• pan gene symbol^?: mnaA
• synonym:
• product: UDP-GlcNAc 2-epimerase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2065 [new locus tag: SAUSA300_RS11375 ]
• symbol: SAUSA300_2065
• product: UDP-GlcNAc 2-epimerase
• replicon: chromosome
• strand: -
• coordinates: 2227584..2228711
• length: 1128
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912922 NCBI
• RefSeq: YP_494711 NCBI
• BioCyc: see SAUSA300_RS11375
• MicrobesOnline: 1293580 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAGATTATGACCATATTTGGAACAAGACCCGAAGCCATAAAGATGGCTCCTTTA
  GTTAAAGCTTTGGAACAAGAAAAAATGCTCGAGCCAATTGTTGTTGTTACAGCACAGCAT
  AGAGAAATGCTTGATTCAGTATTAAGTACCTTTGAAATCAAACCAAAATACGATTTGAAT
  ATTATGAAATCAGGACAGACACTTTCTGAGATAACTTCTAAATCAATCACTCAATTAGAA
  CAAGTGATTCAATTAGAAAAACCAGACATGGTACTTGTACATGGTGATACGATGACGACA
  TTTGCAGGTGGTTTAGCGGCATTTTATAACCAAGTGCCGATTGGACACGTTGAAGCAGGG
  CTTAGAAGTTATGATAAATATTCACCTTTTCCAGAAGAGGTGAATAGACAATTAGTTGGT
  GTATTAGCTGACTTACATTTTGCACCTACTAAAAATGCTGCATCGCATTTGTTAAGTGAA
  GGGAAATATTCAGAAAGTGTTGTTGTCACTGGTAATACTGCGATTGATGCTATGAAATAT
  ACAGTTGATGACAATTATAAATCTAACATCATGGATAAATACCATGACAAAAAATTCATT
  CTTATGACAGCACACCGACGAGAAAATATCGGGAAACCCATGGAAAATATATTTAAAGCG
  GTTAGACGCTTAATAGATGAGTATACTGATTTAGCACTGGTATATCCAATGCATAAAAAC
  CCGAAAGTTCGAGAGGTGGCTCAAAAGATTTTAGGTAGTCATGACAGAATTGAATTGATT
  GAACCTTTGGACGTAGTAGATTTCCATAATTTTGCTAAAAAATCTTATTTTATTTTGACA
  GATTCAGGTGGAATTCAGGAAGAAGCGCCATCATTTAATAAGCCAGTTTTAGTTTTGCGA
  AGTGTTACAGAACGTCCTGAAGGTGTTGAAGCTGGCACATTAAAAGTCATTGGAACAAAT
  AAGCAAAATGTGTATCAAGCTGCAAAAGAATTAATCGATGATGAGAGATTATATCATCAA
  ATGTCTGAAGCATCTAATCCTTACGGTGATGGATTTGCTTCAGAAAGAATAGTTAATCAT
  ATTAAGTATTATTTGAATTTAATCACTGAAAAACCAAGCGATTTCTGA
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  1128
  

⊟Protein

⊟General

• locus tag: SAUSA300_2065 [new locus tag: SAUSA300_RS11375 ]
• symbol: SAUSA300_2065
• description: UDP-GlcNAc 2-epimerase
• length: 375
• theoretical pI: 6.52172
• theoretical MW: 42412.4
• GRAVY: -0.319733

⊟Function

• reaction:
  EC 5.1.3.14?  ExPASy
  UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 529.4)
  and 5 more
  UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 122.5)
  putative cofactor-binding repeat (TIGR03807; HMM-score: 15.7)
  Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 15)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid-A-disaccharide synthase (TIGR00215; EC 2.4.1.182; HMM-score: 13.9)
  conserved hypothetical protein (TIGR03492; HMM-score: 13.7)
• TheSEED  :
  • UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)
• PFAM:
  GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 410.3)
  and 2 more
  Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 12.6)
  GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00832
  • TAT(Tat/SPI): 0.000193
  • LIPO(Sec/SPII): 0.000496
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160011 NCBI
• RefSeq: YP_494711 NCBI
• UniProt: A0A0H2XE45 UniProt

⊟Protein sequence

• MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREMLDSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFAGGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSEGKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGKPMENIFKAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSYFILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDDERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2065 < upp < glyA < SAUSA300_2068

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications