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⊟Summary[edit | edit source]
- pan ID?: SAUPAN000994000
- symbol?: capP
- synonym:
- description?: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
- UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
- capsular polysaccharide biosynthesis protein Cap5P
- capsular polysaccharide biosynthesis protein CapP
- capsular polysaccharide synthesis protein Cap5P
- capsular polysaccharide synthesis UDP-N-acetylglucosamine 2-epimerase CapP, putative
- type 8 capsular polysaccharide synthesis protein Cap8P
- UDP-N-acetylglucosamine 2-epimerase
- UDP-N-acetylglucosamine 2-epimerase Cap5P
- Capsular polysaccharide synthesis enzyme Cap8P
- capsular polysaccharide synthesis enzyme Cap5P
- capsular polysaccharide synthesis enzyme CapP
- UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc-2-epimerase). domain protein
- UDP-N-acetylglucosamine 2-epimerase CapP
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1263219..1270246
- synteny block?: BlockID0005270
- occurrence?: in 100% of 34 strains
capP : capsule polysaccharide precursor synthesis enzyme UDP-N-acetylglucosamine 2-epimerase [1]
• A crystal structure is available : 5ENZ
Staphylococci can produce up to 15 different capsules based on variants of the core trisaccharide N-acetyl-D-mannosaminouronate (ManNAcA) - N-acetyl-L-fucosamine (L-FucNAc) - N-acetyl-D-fucosamine (D-FucNAc). CapP catalyzes the first of two steps in the biosynthesis of UDP-N-acetyl-D-mannosaminouronate (D-ManNAcA), an epimerization at C-2 of UDP-N-acetyl-D-glucosamine to form UDP-N-acetyl-D-mannosamine.
Historically, different capsule types were assigned their own nomenclature (i.e. Cap5P and Cap8P for the two most prevalent capsule types, CP5 and CP8). The CapP protein is 99% identical between Cap5P and Cap8P, provides the same function, and is therefore harmonized as CapP.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0165
08BA02176:
C248_0153 (capP)
11819-97:
MS7_0158
6850:
RSAU_000118 (capP)
71193:
ST398NM01_0178
ECT-R 2:
ECTR2_128
ED133:
SAOV_0111
ED98:
SAAV_0132 (cap5P)
HO 5096 0412:
SAEMRSA15_01300 (capP)
JH1:
SaurJH1_0155
JH9:
SaurJH9_0150
JKD6008:
SAA6008_00142 (cap5P)
JKD6159:
SAA6159_00148 (cap5P)
JSNZ:
JSNZ_000107 (cap8P)
LGA251:
SARLGA251_01380 (capP)
M013:
M013TW_0154
MRSA252:
SAR0166 (capP)
MSHR1132:
SAMSHR1132_01380
MSSA476:
SAS0139
Mu3:
SAHV_0163 (capP)
Mu50:
SAV0164 (capP)
MW2:
MW0139 (cap8P)
RF122:
SAB0105 (capP)
ST398:
SAPIG0178
T0131:
SAT0131_00155 (mreB)
TCH60:
HMPREF0772_10334 (capP)
TW20:
SATW20_01750 (capP)
USA300_TCH1516:
USA300HOU_0178 (cap5P)
VC40:
SAVC_00590
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MCLNFREDNVMKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSI
N315 MCLNFREDNVMKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSI
NCTC8325 ----------MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSI
Newman MCLNFREDNVMKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSI
USA300_FPR3757 MCLNFREDNVMKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSI
**************************************************
COL FDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYH
N315 FDIQADHDLNIMQDQQTLADLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYH
NCTC8325 FDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYH
Newman FDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYH
USA300_FPR3757 FDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYH
*******************.****************************************
COL QIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFIT
N315 QIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFIT
NCTC8325 QIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFIT
Newman QIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFIT
USA300_FPR3757 QIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFIT
************************************************************
COL GNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKD
N315 GNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIFKAVRDLADEYKD
NCTC8325 GNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKD
Newman GNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKD
USA300_FPR3757 GNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKD
*****************************:******************************
COL VVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAP
N315 VVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAP
NCTC8325 VVFIYPMHRNLKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAP
Newman VVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAP
USA300_FPR3757 VVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAP
********** *************************************************
COL TFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGD
N315 TFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGD
NCTC8325 TFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGD
Newman TFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGD
USA300_FPR3757 TFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGD
************************************************************
COL GQASRRICEAIEYYFGLRTDKPDEFVPLRHK
N315 GQASRRICEAIEYYFGLRSDKPDEFVPLRHK
NCTC8325 GQASRRICEAIEYYFGLRTDKPDEFVPLRHK
Newman GQASRRICEAIEYYFGLRTDKPDEFVPLRHK
USA300_FPR3757 GQASRRICEAIEYYFGLRTDKPDEFVPLRHK
******************:************
- ↑ Satsawat Visansirikul, Stephen A Kolodziej, Alexei V Demchenko
Staphylococcus aureus capsular polysaccharides: a structural and synthetic perspective.
Org Biomol Chem: 2020, 18(5);783-798
[PubMed:31922180] [WorldCat.org] [DOI] (I p)