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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004141000
- symbol?: trmK
- synonym:
- description?: tRNA methyltransferase
- tRNA methyltransferase
- methyltransferase, putative
- tRNA (adenine(22)-N(1))-methyltransferase TrmK
- tRNA (adenine-N(1))-methyltransferase
- Putative tRNA-m1A22 methylase
- Bcl-2 family protein
- SAM-dependent methyltransferase
- tRNA (adenine-N(1)-)-methyltransferase TrmK
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4409364..4410041
- synteny block?: BlockID0031710
- occurrence?: in 100% of 34 strains
trmK : tRNA adenosine(22) N(1)-methyltransferase [1]
• A crystal structure is available: 7O4M
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrmK is a methyltransferase specific for adenosines at tRNA position 22 generating 1-methyladenosine ("m1A" or " " "). Staphylococcal tRNAs requiring this modification include: tRNAH1GUG, tRNAYGUA, tRNAEUUC, tRNASGCU, tRNASUGA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1518
08BA02176:
C248_1603
11819-97:
MS7_1577 (trmK)
6850:
RSAU_001425
71193:
ST398NM01_1625
ECT-R 2:
ECTR2_1410
ED133:
SAOV_1560
ED98:
SAAV_1552
HO 5096 0412:
SAEMRSA15_14800
JH1:
SaurJH1_1652
JH9:
SaurJH9_1618
JKD6008:
SAA6008_01529
JKD6159:
SAA6159_01495
JSNZ:
JSNZ_001551
LGA251:
SARLGA251_14660
M013:
M013TW_1575
MRSA252:
SAR1637
MSHR1132:
SAMSHR1132_14000
MSSA476:
SAS1498
Mu3:
SAHV_1547
Mu50:
SAV1560
MW2:
MW1512
RF122:
SAB1432c
ST398:
SAPIG1625
T0131:
SAT0131_01654
TCH60:
HMPREF0772_11580
TW20:
SATW20_15560
USA300_TCH1516:
USA300HOU_1562
VC40:
SAVC_07050
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MISLNNRLTTVSCFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNV
N315 MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNV
NCTC8325 MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNV
Newman MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNV
USA300_FPR3757 MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGIAGEVIQGPFQAAVKNV
************ ***********************************************
COL AANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQS
N315 AANHLVERIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQS
NCTC8325 AANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQS
Newman AANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQS
USA300_FPR3757 AANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQS
***:**:*************:***************************************
COL NIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTELIELSSDELKFGPKLLNNK
N315 NIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEHSTQLIELSSDELKFGPKLLNNK
NCTC8325 NIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTELIELSSDELKFGPKLLNNK
Newman NIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTELIELSSDELKFGPKLLNNK
USA300_FPR3757 NIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTELIELSSDELKFGPKLLNNK
*************************************:**:*******************
COL NEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL
N315 NEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL
NCTC8325 NEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL
Newman NEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL
USA300_FPR3757 NEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL
*********************************************
- ↑ Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Pamela Sweeney, Ashleigh Galliford, Abhishek Kumar, Dinesh Raju, Naveen B Krishna, Emmajay Sutherland, Caitlin J Leo, Gemma Fisher, Roopa Lalitha, Likith Muthuraj, Gladstone Sigamani, Verena Oehler, Silvia Synowsky, Sally L Shirran, Tracey M Gloster, Clarissa M Czekster, Pravin Kumar, Rafael G da Silva
Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m 1A22-tRNA methyltransferase TrmK.
J Biol Chem: 2022, 298(6);102040
[PubMed:35595101] [WorldCat.org] [DOI] (I p)