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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001551
  • pan locus tag?: SAUPAN004141000
  • symbol: JSNZ_001551
  • pan gene symbol?: trmK
  • synonym:
  • product: tRNA (adenine(22)-N(1))-methyltransferase TrmK

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001551
  • symbol: JSNZ_001551
  • product: tRNA (adenine(22)-N(1))-methyltransferase TrmK
  • replicon: chromosome
  • strand: -
  • coordinates: 1587504..1588181
  • length: 678
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATTTCGTTAAATAACCGATTAACGACGGTAAGTCGATTTTTAAAACAAGGTACAATC
    GCGGATATTGGCTCAGATCATGCTTATTTGCCAATCTATGCAATTCAAAACCATTTATGC
    GATTACGGTATTGCAGGAGAAGTTATTCAAGGCCCTTTTCAAGCTGCTGTGAAAAATGTA
    GCTGCAAACCAATTAGTAGATAGAATTGATGTACGTTTAGGGGATGGCTTAAGTGTTATT
    CAACCTGAGGACGTAATCGATAATATCACAATTTGTGGTATGGGCGGACCACTTATTGCT
    AAAATTTTAAAAGATGGGCAAGATAAGTTAAGTCAACATCCAAGGTTGATTTTACAAAGT
    AATATACAAACTGAAAATTTAAGACAAACATTACAACAATTGAATTATGAAATAATTGAT
    GAAATAATAATGGAAGAAAAAGGCCATATTTATGAAATTGTTGTAGCAGAATATTCTACA
    CAATTAATAGAGCTATCATCTGATGAATTGAAATTCGGACCAAAATTGCTGAACAACAAA
    AATGAATATTTTATAAAAAAATGGCAAAGAGAGTTAGAAGCACTTTATCATATTAAATCG
    AAACTCAATACTGAACAACATCATCAACGTTTAGCTCAAATTAATGACGAAATTGCTGTA
    ATTGAAAGGGTGTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001551
  • symbol: JSNZ_001551
  • description: tRNA (adenine(22)-N(1))-methyltransferase TrmK
  • length: 225
  • theoretical pI: 5.32608
  • theoretical MW: 25553.1
  • GRAVY: -0.221333

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.217?  ExPASy
    tRNA (adenine22-N1)-methyltransferase S-adenosyl-L-methionine + adenine22 in tRNA = S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) TrmK; tRNA (adenine(22)-N(1))-methyltransferase (PF04816; HMM-score: 271.3)
    and 3 more
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 164.9)
    CYTH (CL0273) Med18; Med18 protein (PF09637; HMM-score: 13.9)
    no clan defined AAR2_1st; AAR2 N-terminal domain (PF20981; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9995
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0004
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006887
    • TAT(Tat/SPI): 0.000785
    • LIPO(Sec/SPII): 0.000694
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCDYGIAGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIQPEDVIDNITICGMGGPLIAKILKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYSTQLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEIAVIERVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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