⊟Summary[edit | edit source]
- pan ID?: SAUPAN003860000
- symbol?: brxA
- synonym:
- description?: bacilliredoxin BrxA
- bacilliredoxin BrxA
- BrxA/BrxB family bacilliredoxin
- disulfide isomerase, putative
- protein YphP
- YqiW like protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4141829..4142266
- synteny block?: BlockID0029160
- occurrence?: in 100% of 34 strains
brxA (yphP, cvfC4) : protein S-bacillithiol monothiol reductase A [1]
• A crystal structure is available : 7RZB
During conditions of oxidative stress, staphylococci protect free thiols in proteins by forming disulfides with bacillithiol, conceptually equivalent to glutathione. During recovery, bacilliredoxins like BrxA reduce protein-bacillithiol disulfides that are in the S-enantiomeric configuration back to free thiols. Thioredoxins (and oxidized bacillithiol) are then regenerated to an active (reduced) form by bacillithiol disulfide reductase (bdr/YpdA). Bacilliredoxins often display specificity in the target proteins that they reduce; BrxA is very efficient at reducing bacillithiolated glyceraldehyde 3-phosphate dehydrogenase (GapDH).
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADDTTFVVINSTCGCAAGL
N315 MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADDTTFVVINSTCGCAAGL
NCTC8325 MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADDTTFVVINSTCGCAAGL
Newman MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADDTTFVVINSTCGCAAGL
USA300_FPR3757 MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADDTTFVVINSTCGCAAGL
************************************************************
COL ARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAPSSPSYALFKGQDLVYFMP
N315 ARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAPSSPSYALFKGQDLVYFMP
NCTC8325 ARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAPSSPSYALFKGQDLVYFMP
Newman ARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAPSSPSYALFKGQDLVYFMP
USA300_FPR3757 ARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAPSSPSYALFKGQDLVYFMP
************************************************************
COL REFIEGRDINDIAMDLKDAFDENCK
N315 REFIEGRDINDIAMDLKDAFDENCK
NCTC8325 REFIEGRDINDIAMDLKDAFDENCK
Newman REFIEGRDINDIAMDLKDAFDENCK
USA300_FPR3757 REFIEGRDINDIAMDLKDAFDENCK
*************************
- ↑ Mariko Ikuo, Chikara Kaito, Kazuhisa Sekimizu
The cvfC operon of Staphylococcus aureus contributes to virulence via expression of the thyA gene.
Microb Pathog: 2010, 49(1-2);1-7
[PubMed:20347953] [WorldCat.org] [DOI] (I p)Nico Linzner, Vu Van Loi, Verena Nadin Fritsch, Quach Ngoc Tung, Saskia Stenzel, Markus Wirtz, Rüdiger Hell, Chris J Hamilton, Karsten Tedin, Marcus Fulde, Haike Antelmann
Staphylococcus aureus Uses the Bacilliredoxin (BrxAB)/Bacillithiol Disulfide Reductase (YpdA) Redox Pathway to Defend Against Oxidative Stress Under Infections.
Front Microbiol: 2019, 10;1355
[PubMed:31275277] [WorldCat.org] [DOI] (P e)Colin S McHugh, Paul D Cook
Structure of BrxA from Staphylococcus aureus, a bacilliredoxin involved in redox homeostasis in Firmicutes.
Acta Crystallogr F Struct Biol Commun: 2022, 78(Pt 4);144-149
[PubMed:35400666] [WorldCat.org] [DOI] (I p)