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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003341000
- symbol?: pycA
- synonym:
- description?: pyruvate carboxylase
- pyruvate carboxylase
- putative pyruvate carboxylase
- pyruvate carboxylase PycA
- Pyruvate carboxyl transferase
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3707075..3710551
- synteny block?: BlockID0024250
- occurrence?: in 100% of 34 strains
pycA : pyruvate carboxylase [1]
• A crystal structure is available : 3HO8
Conversion of pyruvate to oxaloacetate is an essential step in multiple pathways from the citric acid cycle to gluconeogenesis and lipid synthesis. Staphylococcal pyruvate carboxylase (PC) is a homotetramer with each subunit containing five domains: a biotin carboxyl carrier protein (BCCP) domain which binds to the essential cofactor, a biotin carboxylase (BC) domain which modifies the BCCP-bound biotin to carboxybiotin and a carboxyltransferase (CT) domain which both stabilizes pyruvate as an enolate and releases CO2 locally from carboxybiotin, favoring production of oxaloacetate. The remaining two domains include an allosteric domain (AD) for regulation of catalytic activity and a PC tetramerization (PT) domain to facilitate the macromolecular assembly of the functional homotetramer.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1071 (pycA)
08BA02176:
C248_1141
11819-97:
MS7_1071 (pyc)
6850:
RSAU_000999 (pyc)
71193:
ST398NM01_1111
ECT-R 2:
ECTR2_969 (pyc)
ED133:
SAOV_1056
ED98:
SAAV_1080 (pyc)
HO 5096 0412:
SAEMRSA15_09440
JH1:
SaurJH1_1196
JH9:
SaurJH9_1174
JKD6008:
SAA6008_01069 (pycA)
JKD6159:
SAA6159_00969 (pycA)
LGA251:
SARLGA251_10270
M013:
M013TW_1046
MRSA252:
SAR1088
MSHR1132:
SAMSHR1132_09610
MSSA476:
SAS1049
Mu3:
SAHV_1106 (pycA)
Mu50:
SAV1114 (pycA)
MW2:
MW0997 (pycA)
RF122:
SAB0979
ST398:
SAPIG1111 (pyc)
T0131:
SAT0131_01153 (lacB)
TCH60:
HMPREF0772_12118 (pyc)
TW20:
SATW20_11090
USA300_TCH1516:
USA300HOU_1052 (pycA)
VC40:
SAVC_04735
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 | |
| JSNZ |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.505)
COL ------MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
N315 ------MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
NCTC8325 ------MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
Newman MRSVSLLKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
USA300_FPR3757 ------MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
JSNZ ------MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVG
:*****************************************************
COL SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
N315 SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
NCTC8325 SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
Newman SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
USA300_FPR3757 SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
JSNZ SDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLD
************************************************************
COL MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
N315 MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
NCTC8325 MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
Newman MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
USA300_FPR3757 MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
JSNZ MFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIV
************************************************************
COL REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
N315 REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
NCTC8325 REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
Newman REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
USA300_FPR3757 REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
JSNZ REESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSV
************************************************************
COL QRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
N315 QRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
NCTC8325 QRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
Newman QRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
USA300_FPR3757 QRRHQKVVEVAPSVGLSSTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
JSNZ QRRHQKVVEVVPSVGLSPTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPR
**********.******.******************************************
COL VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
N315 VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
NCTC8325 VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
Newman VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
USA300_FPR3757 VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
JSNZ VQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLN
************************************************************
COL DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
N315 DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
NCTC8325 DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
Newman DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
USA300_FPR3757 DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
JSNZ DFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVR
************************************************************
COL SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIG
N315 SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIG
NCTC8325 SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIG
Newman SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIG
USA300_FPR3757 SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFNIQPSLDRGTKTLEYIG
JSNZ SLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIG
*******************************************:****************
COL NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
N315 NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
NCTC8325 NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
Newman NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
USA300_FPR3757 NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
JSNZ NVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVL
************************************************************
COL LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
N315 LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
NCTC8325 LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
Newman LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
USA300_FPR3757 LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
JSNZ LTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPW
************************************************************
COL ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
N315 ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
NCTC8325 ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
Newman ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
USA300_FPR3757 ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
JSNZ ERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVD
************************************************************
COL QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
N315 QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
NCTC8325 QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
Newman QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
USA300_FPR3757 QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
JSNZ QMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDM
************************************************************
COL AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
N315 AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
NCTC8325 AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
Newman AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
USA300_FPR3757 AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
JSNZ AGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGL
************************************************************
COL TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
N315 TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
NCTC8325 TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
Newman TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
USA300_FPR3757 TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
JSNZ TSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEM
************************************************************
COL PGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
N315 PGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
NCTC8325 PGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
Newman PGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
USA300_FPR3757 PGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
JSNZ PGGQYSNLSQQAKSLVLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDL
*************** ********************************************
COL DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
N315 DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
NCTC8325 DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
Newman DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
USA300_FPR3757 DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
JSNZ DEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVD
************************************************************
COL FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
N315 FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
NCTC8325 FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
Newman FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
USA300_FPR3757 FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
JSNZ FEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGE
************************************************************
COL TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
N315 TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
NCTC8325 TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
Newman TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
USA300_FPR3757 TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
JSNZ TVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADK
************************************************************
COL SNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
N315 SNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
NCTC8325 SNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
Newman SNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
USA300_FPR3757 SNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
JSNZ SNPSHIGAQMPGSVTEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGD
***********************:************************************
COL TIATGDLLIEIEKATD
N315 TIATGDLLIEIEKATD
NCTC8325 TIATGDLLIEIEKATD
Newman TIATGDLLIEIEKATD
USA300_FPR3757 TIATGDLLIEIEKATD
JSNZ TIATGDLLIEIEKATD
****************
- ↑ Song Xiang, Liang Tong
Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction.
Nat Struct Mol Biol: 2008, 15(3);295-302
[PubMed:18297087] [WorldCat.org] [DOI] (I p)Linda P C Yu, Song Xiang, Gorka Lasso, David Gil, Mikel Valle, Liang Tong
A symmetrical tetramer for S. aureus pyruvate carboxylase in complex with coenzyme A.
Structure: 2009, 17(6);823-32
[PubMed:19523900] [WorldCat.org] [DOI] (I p)Amanda J Laseke, Trevor J Boram, Nicholas O Schneider, Jeremy R Lohman, Martin St Maurice
Allosteric Site at the Biotin Carboxylase Dimer Interface Mediates Activation and Inhibition in Staphylococcus aureus Pyruvate Carboxylase.
Biochemistry: 2023, 62(17);2632-2644
[PubMed:37603581] [WorldCat.org] [DOI] (I p)