⊟Summary[edit | edit source]

pan ID^?: SAUPAN003199000
symbol^?: prfC
synonym:
description^?: peptide chain release factor 3
- peptide chain release factor 3
- peptide chain release factor 3 (RF-3)
- peptide chain release factor RF3
strand^?: +
coordinates^?: 3589850..3591458
synteny block^?: BlockID0022860
occurrence^?: in 100% of 34 strains

prfC : peptide chain release factor 3 ^[1]

Ribosomal peptide release has not been extensively characterized in firmicutes and functions are therefore extrapolated from foundational studies in E. coli and pseudomonas. Peptide chain release factor 3 (RF-3), a "type 2" release factor, is structurally and functionally distinct from "type 1" release factors such as RF-1 and RF-2. Whereas Type 1 RFs promote release of completed peptides from the ribosome at stop codons, RF-3 serves a more regulatory and factor-recycling role. RF-3 stimulates dissociation of type 1 RFs from the ribosome after they have finished releasing the completed peptide. This is particularly important at UGA stop codons which effectively sequester RF-2 until it is released by RF-3. RF-3 may also enhance recruitment of type 1 RFs to the ribosome, further increasing the catalytic efficiency of peptide release and ribosome subunit recycling. Finally, RF-3 binds to, and is inhibited by, GTP, suggesting a regulatory role for the phosphorylation state of intracellular guanosine nucleotide pools in the rate of peptide product release and ribosomal recycling.

⊟Orthologs[edit | edit source]

COL:

SACOL1025 (prfC)

N315:

SA0877 (prfC)

NCTC8325:

SAOUHSC_00956 (prfC)

Newman:

NWMN_0890 (prfC)

USA300_FPR3757:

SAUSA300_0921 (prfC)

JSNZ:

JSNZ_000979

04-02981:

SA2981_0974 (prfC)

08BA02176:

C248_1043 (prfC)

11819-97:

MS7_0976 (prfC)

6850:

RSAU_000905 (prfC)

71193:

ST398NM01_1016

ECT-R 2:

ECTR2_875 (prfC)

ED133:

SAOV_0964

ED98:

SAAV_0982

HO 5096 0412:

SAEMRSA15_08500 (prfC)

JH1:

SaurJH1_1037 (prfC)

JH9:

SaurJH9_1018 (prfC)

JKD6008:

SAA6008_00975 (prfC)

JKD6159:

SAA6159_00876

LGA251:

SARLGA251_09350 (prfC)

M013:

M013TW_0945

MRSA252:

SAR0990 (prfC)

MSHR1132:

SAMSHR1132_08670

MSSA476:

SAS0889 (prfC)

Mu3:

SAHV_1014

Mu50:

SAV1021

MW2:

MW0901 (prfC)

RF122:

SAB0886 (prfC)

ST398:

SAPIG1016 (prfC)

T0131:

SAT0131_01055

TCH60:

HMPREF0772_12214 (prfC)

TW20:

SATW20_10170 (prfC)

USA300_TCH1516:

USA300HOU_0978 (prfC)

VC40:

SAVC_04265 (prfC)

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

alignment of orthologues:
CLUSTAL format alignment by MAFFT L-INS-i (v7.307)

COL             MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
N315            MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
NCTC8325        MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
Newman          MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
USA300_FPR3757  MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVE
                ************************************************************

COL             QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
N315            QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
NCTC8325        QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
Newman          QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
USA300_FPR3757  QERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQT
                ************************************************************

COL             LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
N315            LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
NCTC8325        LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
Newman          LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
USA300_FPR3757  LKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGII
                ************************************************************

COL             DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
N315            DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFEQAIEELMLVEEAGEAFDNDALL
NCTC8325        DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
Newman          DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
USA300_FPR3757  DRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALL
                *********************************** ************************

COL             SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
N315            SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
NCTC8325        SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
Newman          SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
USA300_FPR3757  SGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQAN
                ************************************************************

COL             MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
N315            MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
NCTC8325        MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
Newman          MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
USA300_FPR3757  MDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIG
                ************************************************************

COL             LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
N315            LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
NCTC8325        LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
Newman          LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
USA300_FPR3757  LYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAI
                ************************************************************

COL             QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
N315            QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
NCTC8325        QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
Newman          QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
USA300_FPR3757  QYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMN
                ************************************************************

COL             TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
N315            TSRSILVKDRYDDLVFLFENEFATRWFEEKFLEIKLYSLL
NCTC8325        TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
Newman          TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
USA300_FPR3757  TSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL
                ******************************* ********

↑ Alexandros D Petropoulos, Megan E McDonald, Rachel Green, Hani S Zaher
Distinct roles for release factor 1 and release factor 2 in translational quality control.
J Biol Chem: 2014, 289(25);17589-96
[PubMed:24798339] [WorldCat.org] [DOI] (I p)

[1] Alexandros D Petropoulos, Megan E McDonald, Rachel Green, Hani S Zaher
Distinct roles for release factor 1 and release factor 2 in translational quality control.
J Biol Chem: 2014, 289(25);17589-96
[PubMed:24798339] [WorldCat.org] [DOI] (I p)

[1]

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Contents

⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757
JSNZ

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⊟Summary[edit | edit source]

⊟Orthologs[edit | edit source]

⊟Genome Viewer[edit | edit source]

⊟Alignments[edit | edit source]