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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00956
  • pan locus tag?: SAUPAN003199000
  • symbol: prfC
  • pan gene symbol?: prfC
  • synonym:
  • product: peptide chain release factor 3

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00956
  • symbol: prfC
  • product: peptide chain release factor 3
  • replicon: chromosome
  • strand: +
  • coordinates: 930208..931770
  • length: 1563
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAACTTAAAGCAAGAAGTTGAGTCTAGAAAGACTTTTGCGATTATTTCACATCCCGAT
    GCAGGGAAAACAACGTTAACTGAAAAACTATTGTACTTCAGTGGTGCTATTCGTGAAGCG
    GGTACAGTTAAAGGGAAGAAGACTGGTAAATTTGCGACAAGTGACTGGATGAAAGTTGAA
    CAAGAGCGTGGTATTTCTGTAACTAGTTCAGTAATGCAATTTGATTACGATGATTATAAA
    ATCAATATCTTAGATACACCAGGACATGAAGACTTTTCAGAAGATACGTATAGAACATTA
    ATGGCAGTTGACAGTGCTGTCATGGTCATAGACTGTGCAAAAGGTATTGAACCACAAACA
    TTGAAGTTATTTAAAGTTTGTAAAATGCGTGGTATTCCAATCTTTACATTCATTAATAAA
    TTAGACCGAGTAGGTAAAGAACCATTTGAATTATTAGATGAAATCGAAGAGACATTAAAT
    ATTGAAACATACCCTATGAATTGGCCAATTGGTATGGGACAAAGTTTCTTTGGCATCATT
    GATAGAAAGTCTAAAACAATTGAACCATTTAGAGATGAAGAAAATATATTACATTTGAAT
    GATGATTTTGAGTTGGAAGAAGATCATGCAATTACAAATGATAGTGATTTTGAACAAGCG
    ATTGAAGAATTAATGTTGGTTGAAGAAGCGGGTGAAGCCTTTGATAATGACGCGCTGTTG
    AGTGGAGACTTAACACCTGTATTTTTCGGTTCAGCTTTAGCTAACTTTGGTGTACAAAAT
    TTCTTAAATGCATATGTTGATTTTGCGCCAATGCCAAATGCGAGACAAACAAAAGAAGAC
    GTTGAAGTAAGCCCGTTTGATGATTCATTTTCAGGATTTATCTTTAAAATTCAAGCCAAC
    ATGGACCCTAAACACCGTGATAGAATTGCCTTTATGCGTGTCGTTAGTGGTGCATTTGAA
    CGTGGTATGGATGTTACTTTGCAACGTACTAATAAAAAGCAAAAGATCACACGTTCAACG
    TCATTTATGGCAGACGATAAAGAAACTGTGAATCATGCTGTAGCAGGCGATATCATTGGA
    CTATATGATACTGGTAATTATCAAATTGGAGATACTTTAGTTGGTGGAAAACAAACCTAC
    AGTTTCCAAGATTTACCACAATTTACGCCAGAAATTTTTATGAAAGTTTCTGCTAAAAAC
    GTCATGAAACAGAAGCATTTCCATAAAGGTATTGAACAATTAGTACAAGAAGGTGCGATT
    CAATACTATAAAACATTACACACAAACCAAATTATTTTAGGTGCTGTTGGTCAGTTACAA
    TTTGAAGTTTTCGAACATAGAATGAAAAACGAATATAATGTTGATGTTGTTATGGAGCCA
    GTAGGCCGTAAAATTGCACGTTGGATTGAAAATGAAGACCAAATTACAGATAAGATGAAC
    ACATCAAGATCGATTTTAGTGAAAGATAGATATGACGATTTAGTATTCTTATTTGAAAAT
    GAATTTGCAACAAGATGGTTTGAAGAGAAATTCCCTGAAATTAAATTGTATAGTTTACTT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1563

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00956
  • symbol: PrfC
  • description: peptide chain release factor 3
  • length: 520
  • theoretical pI: 4.61861
  • theoretical MW: 59601.2
  • GRAVY: -0.390577

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 647.7)
    and 18 more
    Genetic information processing Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 250.4)
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 123.9)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 123.4)
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 117.3)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 88.2)
    Genetic information processing Protein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 66.5)
    Genetic information processing Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 61.2)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 55.5)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 48.7)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 45.8)
    Genetic information processing Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 40.5)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 28.3)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 24.6)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 17.7)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 12.2)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 11.6)
  • TheSEED  :
    • Peptide chain release factor 3
    Protein Metabolism Protein biosynthesis Translation termination factors bacterial  Peptide chain release factor 3
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 190.4)
    EF-G_C (CL0437) RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 154.5)
    and 7 more
    ALR_C-like (CL0866) EF-G_D2; Elongation factor G domain 2 (PF22042; HMM-score: 82.3)
    GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 53.4)
    EF-G_C (CL0437) EFG_III; Elongation Factor G, domain III (PF14492; HMM-score: 34.7)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 31.3)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 17.9)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 13.4)
    Ras; Ras family (PF00071; HMM-score: 13.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9732
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0238
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013097
    • TAT(Tat/SPI): 0.002964
    • LIPO(Sec/SPII): 0.001929
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMAVDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLNIETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSDFEQAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQTKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQKITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEIFMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKNEYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFEEKFPEIKLYSLL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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