⊟Summary[edit | edit source]
- pan ID?: SAUPAN003192000
- symbol?: —
- synonym:
- description?: RNA ligase, putative
- RNA ligase, putative
- YjcG family protein
- 2-5 RNA ligase
- 2',5' RNA ligase
- 2H phosphoesterase superfamily protein Bsu1186(yjcG)
- conserved protein YjcG
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3584655..3585164
- synteny block?: BlockID0022790
- occurrence?: in 100% of 34 strains
yjcG : 2',3'-cyclic purine 2'-phosphodiesterase [1]
The physiological role of YjcG in firmicutes remains unclear and underexplored. It is established as a virulence factor in the silkworm model, required for virulence factor production, and biochemically characterized as a 2',3'-cyclic purine 2'-phosphodiesterase. However, although staphylococci do use 2',3'-cAMP signaling, the KD for free 2',3'-cGAMP is too high for free cyclic nucleotides to be the natural substrate. Furthermore, YjcG expression is regulated by binding of small RNA RsaE to the 5'-UTR of its mRNA suggesting tight regulatory control. Although to my knowledge this has not yet been proposed, the most likely role for YjcG is in modulation of mRNA stability. Many RNA endonucleases leave a terminal 2',3'-cyclic monophosphate after cleavage. Polynucleotide phosphorylase degrades RNAs with either a free 3'-OH or a 2',3'-cyclic monophosphate but is blocked by 3'-phosphates. Thus, YjcG may stabilize mRNA transcripts cleaved by ribonucleases to terminal 2,'3'-cyclic monophosphates by converting those ends to 3'-phosphates. Indeed, this is the exact mechanism of RNase Y (a.k.a. CvfA) and how it functions as a virulence factor to stabilize hemolysin transcripts.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIKDGDLDSVIEQVRARIN
N315 MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIEDGDLDSVIEQVRARIN
NCTC8325 MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIKDGDLDSVIEQVRARIN
Newman MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIKDGDLDSVIEQVRARIN
USA300_FPR3757 MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIKDGDLDSVIEQVRARIN
*******************************************:****************
COL GIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGEDFYGEAEHVFVPHFTIAQ
N315 GIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGEDFYGEAEHVFVPHFTIAQ
NCTC8325 GIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGEDFYGEAEHVFVPHFTIAQ
Newman GIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGEDFYGEAEHVFVPHFTIAQ
USA300_FPR3757 GIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGEDFYGEAEHVFVPHFTIAQ
************************************************************
COL GLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKWKVIETFKLA
N315 GLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKWKVIETFKLA
NCTC8325 GLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKWKVIETFKLA
Newman GLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKWKVIETFKLA
USA300_FPR3757 GLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKWKVIETFKLA
*************************************************
- ↑ Thomas Geissmann, Clément Chevalier, Marie-Josée Cros, Sandrine Boisset, Pierre Fechter, Céline Noirot, Jacques Schrenzel, Patrice François, François Vandenesch, Christine Gaspin, Pascale Romby
A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation.
Nucleic Acids Res: 2009, 37(21);7239-57
[PubMed:19786493] [WorldCat.org] [DOI] (I p)Shunsuke Numata, Makiko Nagata, Han Mao, Kazuhisa Sekimizu, Chikara Kaito
CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence.
J Biol Chem: 2014, 289(12);8420-31
[PubMed:24492613] [WorldCat.org] [DOI] (I p)Kenta Imae, Yuki Saito, Hayato Kizaki, Hiroki Ryuno, Han Mao, Atsushi Miyashita, Yutaka Suzuki, Kazuhisa Sekimizu, Chikara Kaito
Novel Nucleoside Diphosphatase Contributes to Staphylococcus aureus Virulence.
J Biol Chem: 2016, 291(36);18608-18619
[PubMed:27422825] [WorldCat.org] [DOI] (I p)Yong Zhang, Rym Agrebi, Lauren E Bellows, Jean-François Collet, Volkhard Kaever, Angelika Gründling
Evolutionary Adaptation of the Essential tRNA Methyltransferase TrmD to the Signaling Molecule 3',5'-cAMP in Bacteria.
J Biol Chem: 2017, 292(1);313-327
[PubMed:27881678] [WorldCat.org] [DOI] (I p)Shuhei Mitsutomi, Nobuyoshi Akimitsu, Kazuhisa Sekimizu, Chikara Kaito
Identification of 2H phosphoesterase superfamily proteins with 2'-CPDase activity.
Biochimie: 2019, 165;235-244
[PubMed:31422053] [WorldCat.org] [DOI] (I p)