From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02270
  • pan locus tag?: SAUPAN005287000
  • symbol: SAOUHSC_02270
  • pan gene symbol?: nrgA
  • synonym:
  • product: ammonium transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02270
  • symbol: SAOUHSC_02270
  • product: ammonium transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2100721..2101971
  • length: 1251
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
    CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTC
    ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
    ATTAGTTTTGGGAATGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
    GTAGGTTTTGCGACTCAAGAAGATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
    TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
    ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTTGTATACAGTCCA
    GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
    GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
    GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
    GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
    AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
    TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
    GCATTAGCAGGATTAGTTGTCATTACTCCTGCAGCAGGATATGTAACATATCTTAGTGCA
    ACAATAATGGCTTTAATAGGAGGTATCTGTTGTTATATTGTCATTAATTACATCAAGGTA
    AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
    GGTGCTGTTTTAACAGCAGTTTTCCAAAGTAAAAAAGCCAATCCTGACATTGAGAATGGC
    TTTATTTATACTGGTGACATACATATTATACTTGTACAAATATTATGTGTAACAGCAGTT
    GTAATTTTTAGTATAGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
    TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
    GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02270
  • symbol: SAOUHSC_02270
  • description: ammonium transporter
  • length: 416
  • theoretical pI: 7.37062
  • theoretical MW: 45478.6
  • GRAVY: 0.852885

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds ammonium transporter (TIGR00836; HMM-score: 405.2)
    and 1 more
    probable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.7)
  • TheSEED  :
    • Ammonium transporter NrgA
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Ammonium transporter
  • PFAM:
    no clan defined Ammonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 375.8)
    and 1 more
    Glycine-zipper (CL0500) Gly-zipper_YMGG; YMGG-like Gly-zipper (PF13441; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.020192
    • TAT(Tat/SPI): 0.000994
    • LIPO(Sec/SPII): 0.028482
  • predicted transmembrane helices (TMHMM): 11

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CodY* (repression) regulon, GlnR* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 
    GlnR*(TF)important in Nitrogen assimilation; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_02270&oldid=95996"