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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001997
- pan locus tag?: SAUPAN005287000
- symbol: JSNZ_001997
- pan gene symbol?: nrgA
- synonym:
- product: ammonium transporter
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001997
- symbol: JSNZ_001997
- product: ammonium transporter
- replicon: chromosome
- strand: -
- coordinates: 2010493..2011743
- length: 1251
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1201ATGAATCTTAACGATACGATATTTATGTTTTTGTGTACATTATTAGTTTGGTTAATGACA
CCAGGATTAAGTTTATTTTATGGTGGGTTAGTTCAATCTAAAAATGCGCTTAATACTGTC
ATGCAAAGTATGGCAGCAATTGTGCTTGTTACATTTGTATGGATAACAGTTGGTTTTACA
ATTAGTTTTGGGGAAGGGAATTTATGGTTCGGAAATTGGGAATATACTTTTCTTAATCAT
GTAGGTTTTGCGACTCAAGAAAATATTAGCCCACATATTCCTTTCGCTTTGTTTATGTTA
TTTCAAATGATGTTTTGTACGATTGCAATTTCTATTTTATCTGGTTCAATCGCTGAGAAA
ATGAAGTTTATTCCTTATTTATTATTCGTAGTAATATGGACTGCTCTAGTATACAGTCCA
GTAGCACATTGGGTTTGGGGCGGCGGTTGGATTAACAAACTCGGTGTATTAGATTTCGCT
GGAGGTACGGTTGTTCATATTACATCAGGTGTTTCTGGTTTAGTATTAGCTATTATGATT
GGAAAAGGAAACAAACATTCTGAATCAACACCACATAATCTTATCATTACGTTGATTGGC
GGTATATTCGTGTGGATTGGTTGGTATGGATTTAATGTAGGTAGTGCTTTTACATTTGAT
AATATTGCGATGCTTGCATTTACAAATACTGTCATTTCAGCCAGTGCAGGTGCTATAGGT
TGGTTAATTTTAGAATATATTTTTAAAAAGACGACAAGTTTACTTGGACTTTTACTCGGT
GCATTAGCTGGTTTAGTTGTCATTACACCTGCAGCAGGATACGTGACATATCTTAGTGCA
ACAATCATGGCAATGATCGGCGGTATCTGTTGCTATATCGTTATTAATTACATCAAGGTT
AAACTAAAATATCATGATGCATTAGATGCATTTGGTATTCATGGTGTTGGTGGTATTATT
GGTGCAGTATTAACAGCCTTTTTCCAAAGTAAAAAGGCAAATCCTGACATTGAGAATGGC
TTTATTTATACTGGTGACATACATATTGTACTTGTACAAATATTATGTGTAACAGCAGTT
GTAATTTTTAGTATCGTCATGACGTTTATTATTGCGAAAGTAATTAAATTAATTACACCA
TTATCTGTTACGGAACAAGAAACGAATATAGGATTAGACAAGATTGTTCACGGTGAACAT
GCTTACTTTGAAGGTGAGCTAAATAGATTCAATAAACATATTCGATATTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001997
- symbol: JSNZ_001997
- description: ammonium transporter
- length: 416
- theoretical pI: 7.37067
- theoretical MW: 45544.7
- GRAVY: 0.844231
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds ammonium transporter (TIGR00836; HMM-score: 405.1)and 1 moreprobable ammonium transporter, marine subtype (TIGR03644; HMM-score: 196.5)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: no clan defined Ammonium_transp; Ammonium Transporter Family (PF00909; HMM-score: 377.6)and 1 morePhage_holin_6_1; Bacteriophage holin of superfamily 6 (Holin_LLH) (PF09682; HMM-score: 7.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9996
- Cell wall & surface Score: 0
- Extracellular Score: 0.0004
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.019357
- TAT(Tat/SPI): 0.000918
- LIPO(Sec/SPII): 0.025955
- predicted transmembrane helices (TMHMM): 11
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMAAIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQENISPHIPFALFMLFQMMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSATIMAMIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAFFQSKKANPDIENGFIYTGDIHIVLVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGEHAYFEGELNRFNKHIRY
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulators: GlnR* (repression) regulon, CodY (repression) regulon
GlnR* (TF) important in Nitrogen assimilation; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026) CodY (TF) important in Amino acid metabolism; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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