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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01582
  • pan locus tag?: SAUPAN001246000
  • symbol: SAOUHSC_01582
  • pan gene symbol?: int1
  • synonym:
  • product: bacteriophage integrase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01582
  • symbol: SAOUHSC_01582
  • product: bacteriophage integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 1507292..1508497
  • length: 1206
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTGGATTGAAAAATTTAAAAACAAAAATAACGAAACTAAATACAGATACTACGAGAAG
    TATAAAGATCCATACACAGATAAATGGAAGCGCGTAAGTGTTGTGTTGAACAAGAATACA
    AAACAATCACAAAAAGAAGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTAAAC
    AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGCTATTAGATGAATGGCTT
    GAATATCATATAAAAACATCAGGTTCAAAGTTGACTACTCTTAATAATATAAAAATAAGA
    ATTAAAAACATTAAACGATACTGCTCTGAGAACTTGCTTTTAAACAAACTTGATACAAAA
    TATATGCAGATATTTATTAATAAATTATCAGATATCTATTCTCAAAATCAAGTAACCCGT
    CAACTCGGAGATATGAAAGGAGCTATTAAATATGCAGTTAAATTTTACAATTATCCAAAT
    GAATATTTGTTAACTAATGTCAAAATTCCTAAAAGAAGAAAAACAATAGAGGATATCGAA
    AAAGATGAATCTAAAATGTACAACTATTTAGAAATGAACCAAGTCCTACAGATACGTGAT
    CATATACTAAATGATAATAAGTTACAAAAGCGAAATCGCATTTTAATTGCCAGCATCTTA
    GAAGTACAGGCTTTAACTGGTATGCGCATAGGAGAACTACAAGCACTGCAGGAAAAAGAT
    ATAGATTTATTAAACAAAACTATCAATATAACAGGTACAATTCACCGCATTAAATACGAG
    AAAGGATTCGGATACAAAGACACTACAAAGACTATAAGTTCAAAAAGAAGTATCAGCATC
    AATTCTAGAACCGTAGAAATTTTTAAAAAGATAATACTGGAAAACAAAATGTTGAAAAGA
    TGGAATTCGAGCTATGTTGACAGAGGGTTCATATTCACAACAAAAAAAGGGAATCCTTTA
    TGTAATAATCAAATCGCCGGTGTGCTTAAGAAAACTACAAAAGCTTTAAATATGAATAAG
    AAAGTTACCACGCACACATTTAGACATACACACATAACTTTATTAGTAGAAATGAATGTT
    TCTTTAAAAGCAATTATGAAAAGGGTAGGACATGTAGATGAAAAAACAACCATTCGCATA
    TATACTCATGTAACTGAAAAAATGGATAGAGAACTAACTCAAAAACTCGAAAACATTCCA
    AGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01582
  • symbol: SAOUHSC_01582
  • description: bacteriophage integrase
  • length: 401
  • theoretical pI: 10.4793
  • theoretical MW: 47482
  • GRAVY: -0.701746

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 79.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 69.4)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 47.7)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 47.7)
  • TheSEED  :
    • Phage integrase
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 94.9)
    and 1 more
    HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011252
    • TAT(Tat/SPI): 0.00039
    • LIPO(Sec/SPII): 0.002927
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIKNIKRYCSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLQKRNRILIASILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEKGFGYKDTTKTISSKRSISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigH* regulon
    SigH*(sigma factor)controlling competence and phage integrase genes;  [3]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Liang Tao, Xiaoqian Wu, Baolin Sun
    Alternative sigma factor sigmaH modulates prophage integration and excision in Staphylococcus aureus.
    PLoS Pathog: 2010, 6(5);e1000888
    [PubMed:20485515] [WorldCat.org] [DOI] (I e)
  4. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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