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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
- pan locus tag?: SAUPAN001246000
- symbol: SAUSA300_1438
- pan gene symbol?: int1
- synonym:
- product: phiSLT ORF401-like protein, integrase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
- symbol: SAUSA300_1438
- product: phiSLT ORF401-like protein, integrase
- replicon: chromosome
- strand: +
- coordinates: 1590733..1591938
- length: 1206
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913265 NCBI
- RefSeq: YP_494135 NCBI
- BioCyc: see SAUSA300_RS07855
- MicrobesOnline: 1292953 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGTGGATTGAAAAATTTAAAAACAAAAATAACGAAACTAAATACAGATACTACGAGAAG
TATAAAGATCCATACACAGATAAATGGAAGCGCGTAAGTGTTGTGTTGAACAAGAATACA
AAACAATCTCAAAAAGAAGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTGAAC
AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGCTATTAGATGAATGGCTT
GAATATCATATAAAAACATCAGGTTCAAAGTTGACTACTCTTAATAATATAAAAATAAGA
ATTAGAAACATTAAACGATACAGCTCTGAGAACTTGCTTTTAAACAAACTAGATACAAAA
TATATGCAGATATTTATTAATAAATTATCAGATATCTATTCTCAAAATCAAGTAACCCGT
CAACTCGGAGATATGAAAGGAGCTATTAAATATGCAGTTAAATTTTACAATTATCCAAAT
GAATATTTGTTAACTAATGTCAAAATTCCTAAAAGAAGAAAAACAATAGAGGATATCGAA
AAAGATGAATCTAAAATGTACAACTATTTAGAAATGAACCAAGTCCTACAGATACGTGAT
CATATACTAAATGATAATAAGTTACACAAGCGAAATCGCATTTTAATTGCCAGCATCTTA
GAAGTACAGGCTTTAACTGGTATGCGCATAGGAGAACTACAAGCACTGCAGGAAAAAGAT
ATAGATTTATTAAACAAAACTATCAATATAACAGGTACAATTCACCGCATTAAATACGAG
GAAGGATTCGGATACAAAGACACTACAAAGACTATAAGTTCAAAAAGAAGTATCAGCATC
AATTCTAGAACCGTAGAAATTTTTAAAAAGATAATACTGGAAAACAAAATGTTGAAAAGA
TGGAATTCGAGCTATGTTGACAGAGGGTTCATATTCACAACAAAAAAAGGGAATCCTTTA
TGTAATAATCAAATCGCCGGTGTGCTTAAGAAAACTACAAAAGCTTTAAATATGAATAAG
AAAGTTACCACGCACACATTTAGACATACACACATAACTTTATTAGTAGAAATGAATGTT
TCTTTAAAAGCAATTATGAAAAGGGTAGGACATGTAGATGAAAAAACAACCATTCGCATA
TATACTCATGTAACTGAAAAAATGGATAGAGAACTAACTCAAAAACTCGAAAACATTCCA
AGTTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
- symbol: SAUSA300_1438
- description: phiSLT ORF401-like protein, integrase
- length: 401
- theoretical pI: 10.4693
- theoretical MW: 47503.9
- GRAVY: -0.709726
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 76.9)DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 68.1)and 3 moreDNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 48.6)Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 48.6)Cellular processes Sporulation and germination sporulation protein YabP (TIGR02892; HMM-score: 13.8)
- TheSEED :
- Phage integrase
- PFAM: DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 94.4)and 1 moreHIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 13.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.011252
- TAT(Tat/SPI): 0.00039
- LIPO(Sec/SPII): 0.002927
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIRNIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- data available for NCTC8325
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
This is the integrase for prophage ΦFPR2. It is a site-specific class Sa2int integrase that recognizes the 29-bp attB sequence AAATAAACATATCGTCATAATGTGATGGT and catalyzes either integration or excision of a prophage, typically between two copies of the attB sequence. The classical attB sequence for Sa2int prophage is located within a hypothetical lipoprotein found immediately downstream of the srrB staphylococcal respiratory response sensor histidine kinase. However, the integrase can accommodate an impressive number of mismatches and can integrate in several other locations such as within lacG (GTATTCCAATCACATCATAACATGATGGT), sea (CATTTTGTTCATCATCATTATAATATTTA) or elsewhere, albeit at lower efficiency. Some S. aureus strains will have two similar Sa2int prophage at both lacG and srrB integration sites. Sa2int prophage frequently carry coding sequences for the lukFPV lukSPV Panton-Valentine leukocidins.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Christiane Goerke, Roman Pantucek, Silva Holtfreter, Berit Schulte, Manuel Zink, Dorothee Grumann, Barbara M Bröker, Jiri Doskar, Christiane Wolz
Diversity of prophages in dominant Staphylococcus aureus clonal lineages.
J Bacteriol: 2009, 191(11);3462-8
[PubMed:19329640] [WorldCat.org] [DOI] (I p)Douglas R Deutsch, Bryan Utter, Kathleen J Verratti, Heike Sichtig, Luke J Tallon, Vincent A Fischetti
Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus.
Front Microbiol: 2018, 9;1406
[PubMed:30013526] [WorldCat.org] [DOI] (P e)S Narita, J Kaneko, J Chiba, Y Piémont, S Jarraud, J Etienne, Y Kamio
Phage conversion of Panton-Valentine leukocidin in Staphylococcus aureus: molecular analysis of a PVL-converting phage, phiSLT.
Gene: 2001, 268(1-2);195-206
[PubMed:11368915] [WorldCat.org] [DOI] (P p)Christiane Wirtz, Wolfgang Witte, Christiane Wolz, Christiane Goerke
Insertion of host DNA into PVL-encoding phages of the Staphylococcus aureus lineage ST80 by intra-chromosomal recombination.
Virology: 2010, 406(2);322-7
[PubMed:20708208] [WorldCat.org] [DOI] (I p)