PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
• pan locus tag^?: SAUPAN001246000
• symbol: SAUSA300_1438
• pan gene symbol^?: int1
• synonym:
• product: phiSLT ORF401-like protein, integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
• symbol: SAUSA300_1438
• product: phiSLT ORF401-like protein, integrase
• replicon: chromosome
• strand: +
• coordinates: 1590733..1591938
• length: 1206
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913265 NCBI
• RefSeq: YP_494135 NCBI
• BioCyc: see SAUSA300_RS07855
• MicrobesOnline: 1292953 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTGGATTGAAAAATTTAAAAACAAAAATAACGAAACTAAATACAGATACTACGAGAAG
  TATAAAGATCCATACACAGATAAATGGAAGCGCGTAAGTGTTGTGTTGAACAAGAATACA
  AAACAATCTCAAAAAGAAGCAATGTTTCGTTTAGAAGAAAAAATAAAAGAAAAACTGAAC
  AACAAGTCGTCAAGCGAATTAAAAACTTTGACTTTTCACGCGCTATTAGATGAATGGCTT
  GAATATCATATAAAAACATCAGGTTCAAAGTTGACTACTCTTAATAATATAAAAATAAGA
  ATTAGAAACATTAAACGATACAGCTCTGAGAACTTGCTTTTAAACAAACTAGATACAAAA
  TATATGCAGATATTTATTAATAAATTATCAGATATCTATTCTCAAAATCAAGTAACCCGT
  CAACTCGGAGATATGAAAGGAGCTATTAAATATGCAGTTAAATTTTACAATTATCCAAAT
  GAATATTTGTTAACTAATGTCAAAATTCCTAAAAGAAGAAAAACAATAGAGGATATCGAA
  AAAGATGAATCTAAAATGTACAACTATTTAGAAATGAACCAAGTCCTACAGATACGTGAT
  CATATACTAAATGATAATAAGTTACACAAGCGAAATCGCATTTTAATTGCCAGCATCTTA
  GAAGTACAGGCTTTAACTGGTATGCGCATAGGAGAACTACAAGCACTGCAGGAAAAAGAT
  ATAGATTTATTAAACAAAACTATCAATATAACAGGTACAATTCACCGCATTAAATACGAG
  GAAGGATTCGGATACAAAGACACTACAAAGACTATAAGTTCAAAAAGAAGTATCAGCATC
  AATTCTAGAACCGTAGAAATTTTTAAAAAGATAATACTGGAAAACAAAATGTTGAAAAGA
  TGGAATTCGAGCTATGTTGACAGAGGGTTCATATTCACAACAAAAAAAGGGAATCCTTTA
  TGTAATAATCAAATCGCCGGTGTGCTTAAGAAAACTACAAAAGCTTTAAATATGAATAAG
  AAAGTTACCACGCACACATTTAGACATACACACATAACTTTATTAGTAGAAATGAATGTT
  TCTTTAAAAGCAATTATGAAAAGGGTAGGACATGTAGATGAAAAAACAACCATTCGCATA
  TATACTCATGTAACTGAAAAAATGGATAGAGAACTAACTCAAAAACTCGAAAACATTCCA
  AGTTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1438 [new locus tag: SAUSA300_RS07855 ]
• symbol: SAUSA300_1438
• description: phiSLT ORF401-like protein, integrase
• length: 401
• theoretical pI: 10.4693
• theoretical MW: 47503.9
• GRAVY: -0.709726

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 76.9)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 68.1)
  and 3 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 48.6)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 48.6)
  Cellular processes Cellular processes Sporulation and germination sporulation protein YabP (TIGR02892; HMM-score: 13.8)
• TheSEED  :
  • Phage integrase
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 94.4)
  and 1 more
  HIT (CL0265) DcpS_C; Scavenger mRNA decapping enzyme C-term binding (PF11969; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011252
  • TAT(Tat/SPI): 0.00039
  • LIPO(Sec/SPII): 0.002927
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160197 NCBI
• RefSeq: YP_494135 NCBI
• UniProt: A0A0H2XE22 UniProt

⊟Protein sequence

• MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQKEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIRNIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPNEYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIASILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKRSISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTKALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDRELTQKLENIPS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications