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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01472
  • pan locus tag?: SAUPAN003915000
  • symbol: SAOUHSC_01472
  • pan gene symbol?: dinG
  • synonym:
  • product: DnaQ family exonuclease/DinG family helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01472
  • symbol: SAOUHSC_01472
  • product: DnaQ family exonuclease/DinG family helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 1427924..1430617
  • length: 2694
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGTATGGCAACCTATGCCGTTGTGGATTTGGAAACAACAGGCAACCAATTAGATTTT
    GACGATATCATTCAAATTGGTATAACGTTTGTGCGCAATAACCAAATTATTGATACATAT
    CATTCTATGATTAGAACAAATTTAGAGATTCCGCCGTTCATTCAAGCGTTAACATCCATT
    GAAGAAAATATGCTACAACAAGCGCCATATTTTAATCAAGTTGCACAAGAAATCTACGAT
    AAGATAAAAGATTGCATTTTTGTAGCTCATAATGTAGATTTTGATTTGAATTTTATTAAA
    AAGGCATTTAAAGATTGTAATATTCAATATCGTCCCAAAAAAGTTATAGATACACTAGAA
    ATCTTTAAAATAGCTTTTCCTACAGATAAAAGCTATCAATTAAGTGAATTAGCAGAGGCA
    CATGGTATTACCTTAGCAAATGCCCATCGAGCTGACGAAGATGCCGCTACTACTGCTAAA
    TTGATGATATTAGCTTTTGAAAAATTTGAAAAGTTACCGTTAGATACTTTGAAGCAATTA
    TATTATTTAAGTAAACAGTTGAAATACGATTTGTATGATATTTTCTTTGAGATGGTTAGG
    CAATATGATGCAAAGCCATTGGATAAATCGTACGAAAAGTTCGAACAGATTATCTATCGT
    AAGCAAGTTGATTTTAAAAAGCCGACAACAAATTATAATGGCAGCTTGAAGTCTTTATAT
    AGCAAAGCTGTAGATCAACTTGGCTTAACATATCGACCGCAACAATTATATTTAGCGGAA
    ACTATATTAGATCAGCTCATGCATAGTGAAAAAGCAATGATTGAAGCATCACTAGGCAGT
    GGTAAATCATTAGCATATTTACTTGCGGCCTTGATGTATAATATTGAAACTGGGAAGCAT
    GTCATGATTTCTACCAATACTAAATTACTGCAAAGTCAATTGTTAGAAAAAGATATTCCA
    GCAATGAATGAAGCGTTGAATTTTAAAATTAATGCACTATTGATTAAAAGTAAAAGTGAT
    TACATTTCATTGGGGCTTATTAGTCAAATTTTAAAAGATGACACAAGTAATTACGAAGTG
    AATATCTTGAAAATGCAATTGCTGATTTGGATTACAGAGACGCCGTCCGGAGATATACAG
    GAATTAAATTTAAAAGGTGGTCAAAAGATGTATTTTGACCAGAAAATTGAAACATATGTA
    CCGGCACGTCACGATGTTCATTATTATAATTTTATTAAGCGAAATGCACAGAACATTCAA
    ATTGGTATTACAAATCATGCACATTTAATTCATTCTGATGTTGAAAATTCAATATATCAA
    TTGTTTGATGATTGTATAGTGGATGAAGCACATCGCTTACCAGACTATGCCTTAAATCAA
    GTGACAAATGAGTTGAGTTATGCTGATATTAAATATCAGCTTGGTTTGATTGGTAAAAAC
    GAAAATGAAAAATTGTTAAAGGCAATTGATCAACTGGAAAAGCAGCGAATTTTGGAAAAA
    TTAGATATTGCACCAATTGATATTTTTGGCTTGAAGGCTAGTATGAATGAGATTCATGAG
    TTAAATGAGCAATTGTTCTCAACGATATTTACGATTATCAATGATTCTGATGTTTATGAT
    GATGATATTCATCGCTTCCATAATGTATTTACTTTTGAAACAAAAGACATTTTGAAGGAT
    TTACATGCGATTATCGATAAGTTAAATAAAACGCTAGAAATATTCAATGGCATTAGTCAT
    AAAACAGTTAAATCATTACGGAAACAGTTGTTGTATTTAAAAGATAAATTTAAAAATATC
    GAACAAAGTTTAAAAGCTGGACATACTAGTTTCATTTCGATTAAAAATTTATCTCAAAAA
    TCAACAATACGTTTATATGTGAAAGATTACGCTGTAAAAGATGTATTAACGAAACAAGTA
    TTGGAAAAATTTAAATCACTCATTTTTATATCTGGTACTTTAAAATTTAATCATTCGTTT
    GAAGCTTTTAAACAGTTATTCAACAAAGATGTTCACTTTAATACATTTGAAGTTAACACG
    TCGTTACAAAGTGCAAAAAATACAAGTGTATTTATACCGAGTGATGTAGCATCTTATCAA
    TATAAAAATATAGATGAGTATGTAGCATCGATTGTAAGCTATATTATTGAATATACGACT
    ATAACGTCATCAAAATGTTTAGTCTTATTTACGAGTTATAAAATGATGCATATGGTACAA
    GATATGCTAAATGAATTGCCAGAATTTGAAGATTATGTTGTATTAACACAACAGCAGAAC
    CAAAATTATAAAATAGTACAACAGTTTAATAATTTTGATAAGGCCATACTGCTTGGTACG
    TCAACATTTTTTGAAGGTTTTGATTTTCAAGCAAATGGTATTAAATGTGTGATGATAGCG
    AAGTTACCGTTTATGAACAAGCATAATGCAAAATATTGGCTTATGGATTCTGAATTCACT
    TCAACTTTCAAGGAATATGTATTACCTGATGCAGTTACACGTTTTAGACAAGGACTTGGA
    AGATTAATTAGAAATGAAAATGATCGCGGCATAATAGTTTCATTTGATGATCGACTCATA
    AACAGTAATTATAAAAACTTTTTTGAACAAACACTTGAAAATTATCGTCAGAAAAAAGGC
    GACATTCAACAGTTTGGAAAATTATTAAGACAAATTCAAAAAAAGAAAAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2694

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01472
  • symbol: SAOUHSC_01472
  • description: DnaQ family exonuclease/DinG family helicase
  • length: 897
  • theoretical pI: 6.83206
  • theoretical MW: 104217
  • GRAVY: -0.297101

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.12?  ExPASy
    DNA helicase ATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 1269.1)
    and 6 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 139.1)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 94.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 60.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair helicase (rad3) (TIGR00604; EC 3.6.4.-; HMM-score: 57.1)
    CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 47.5)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 17.9)
  • TheSEED  :
    • DinG family ATP-dependent helicase YoaA
    DNA Metabolism DNA repair DNA repair, bacterial DinG and relatives  DinG family ATP-dependent helicase YoaA
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C_2; Helicase C-terminal domain (PF13307; HMM-score: 112.3)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 91.3)
    and 5 more
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 22.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 20.7)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 18.7)
    no clan defined HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.6)
    DUF615; Protein of unknown function (DUF615) (PF04751; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002601
    • TAT(Tat/SPI): 0.00012
    • LIPO(Sec/SPII): 0.000314
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMIRTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKAFKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAKLMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQIIYRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEASLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALLIKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYFDQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEAHRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDIFGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIIDKLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

P M Dunman, E Murphy, S Haney, D Palacios, G Tucker-Kellogg, S Wu, E L Brown, R J Zagursky, D Shlaes, S J Projan
Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci.
J Bacteriol: 2001, 183(24);7341-53
[PubMed:11717293] [WorldCat.org] [DOI] (P p)

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