PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1366 [new locus tag: NWMN_RS07695 ]
• pan locus tag^?: SAUPAN003915000
• symbol: NWMN_1366
• pan gene symbol^?: dinG
• synonym:
• product: DnaQ family exonuclease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1366 [new locus tag: NWMN_RS07695 ]
• symbol: NWMN_1366
• product: DnaQ family exonuclease
• replicon: chromosome
• strand: -
• coordinates: 1530769..1533462
• length: 2694
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331760 NCBI
• RefSeq: YP_001332400 NCBI
• BioCyc:
• MicrobesOnline: 3706918 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGTATGGCAACCTATGCCGTTGTGGATTTGGAAACAACAGGCAACCAATTAGATTTT
  GACGATATCATTCAAATTGGTATAACGTTTGTGCGCAATAACCAAATTATTGATACATAT
  CATTCTATGATTAGAACAAATTTAGAGATTCCGCCGTTCATTCAAGCGTTAACATCCATT
  GAAGAAAATATGCTACAACAAGCGCCATATTTTAATCAAGTTGCACAAGAAATCTACGAT
  AAGATAAAAGATTGCATTTTTGTAGCTCATAATGTAGATTTTGATTTGAATTTTATTAAA
  AAGGCATTTAAAGATTGTAATATTCAATATCGTCCCAAAAAAGTTATAGATACACTAGAA
  ATCTTTAAAATAGCTTTTCCTACAGATAAAAGCTATCAATTAAGTGAATTAGCAGAGGCA
  CATGGTATTACCTTAGCAAATGCCCATCGAGCTGACGAAGATGCCGCTACTACTGCTAAA
  TTGATGATATTAGCTTTTGAAAAATTTGAAAAGTTACCGTTAGATACTTTGAAGCAATTA
  TATTATTTAAGTAAACAGTTGAAATACGATTTGTATGATATTTTCTTTGAGATGGTTAGG
  CAATATGATGCAAAGCCATTGGATAAATCGTACGAAAAGTTCGAACAGATTATCTATCGT
  AAGCAAGTTGATTTTAAAAAGCCGACAACAAATTATAATGGCAGCTTGAAGTCTTTATAT
  AGCAAAGCTGTAGATCAACTTGGCTTAACATATCGACCGCAACAATTATATTTAGCGGAA
  ACTATATTAGATCAGCTCATGCATAGTGAAAAAGCAATGATTGAAGCATCACTAGGCAGT
  GGTAAATCATTAGCATATTTACTTGCGGCCTTGATGTATAATATTGAAACTGGGAAGCAT
  GTCATGATTTCTACCAATACTAAATTACTGCAAAGTCAATTGTTAGAAAAAGATATTCCA
  GCAATGAATGAAGCGTTGAATTTTAAAATTAATGCACTATTGATTAAAAGTAAAAGTGAT
  TACATTTCATTGGGGCTTATTAGTCAAATTTTAAAAGATGACACAAGTAATTACGAAGTG
  AATATCTTGAAAATGCAATTGCTGATTTGGATTACAGAGACGCCGTCCGGAGATATACAG
  GAATTAAATTTAAAAGGTGGTCAAAAGATGTATTTTGACCAGAAAATTGAAACATATGTA
  CCGGCACGTCACGATGTTCATTATTATAATTTTATTAAGCGAAATGCACAGAACATTCAA
  ATTGGTATTACAAATCATGCACATTTAATTCATTCTGATGTTGAAAATTCAATATATCAA
  TTGTTTGATGATTGTATAGTGGATGAAGCACATCGCTTACCAGACTATGCCTTAAATCAA
  GTGACAAATGAGTTGAGTTATGCTGATATTAAATATCAGCTTGGTTTGATTGGTAAAAAC
  GAAAATGAAAAATTGTTAAAGGCAATTGATCAACTGGAAAAGCAGCGAATTTTGGAAAAA
  TTAGATATTGCACCAATTGATATTTTTGGCTTGAAGGCTAGTATGAATGAGATTCATGAG
  TTAAATGAGCAATTGTTCTCAACGATATTTACGATTATCAATGATTCTGATGTTTATGAT
  GATGATATTCATCGCTTCCATAATGTATTTACTTTTGAAACAAAAGACATTTTGAAGGAT
  TTACATGCGATTATCGATAAGTTAAATAAAACGCTAGAAATATTCAATGGCATTAGTCAT
  AAAACAGTTAAATCATTACGGAAACAGTTGTTGTATTTAAAAGATAAATTTAAAAATATC
  GAACAAAGTTTAAAAGCTGGACATACTAGTTTCATTTCGATTAAAAATTTATCTCAAAAA
  TCAACAATACGTTTATATGTGAAAGATTACGCTGTAAAAGATGTATTAACGAAACAAGTA
  TTGGAAAAATTTAAATCACTCATTTTTATATCTGGTACTTTAAAATTTAATCATTCGTTT
  GAAGCTTTTAAACAGTTATTCAACAAAGATGTTCACTTTAATACATTTGAAGTTAACACG
  TCGTTACAAAGTGCAAAAAATACAAGTGTATTTATACCGAGTGATGTAGCATCTTATCAA
  TATAAAAATATAGATGAGTATGTAGCATCGATTGTAAGCTATATTATTGAATATACGACT
  ATAACGTCATCAAAATGTTTAGTCTTATTTACGAGTTATAAAATGATGCATATGGTACAA
  GATATGCTAAATGAATTGCCAGAATTTGAAGATTATGTTGTATTAACACAACAGCAGAAC
  CAAAATTATAAAATAGTACAACAGTTTAATAATTTTGATAAGGCCATACTGCTTGGTACG
  TCAACATTTTTTGAAGGTTTTGATTTTCAAGCAAATGGTATTAAATGTGTGATGATAGCG
  AAGTTACCGTTTATGAACAAGCATAATGCAAAATATTGGCTTATGGATTCTGAATTCACT
  TCAACTTTCAAGGAATATGTATTACCTGATGCAGTTACACGTTTTAGACAAGGACTTGGA
  AGATTAATTAGAAATGAAAATGATCGCGGCATAATAGTTTCATTTGATGATCGACTCATA
  AACAGTAATTATAAAAACTTTTTTGAACAAACACTTGAAAATTATCGTCAGAAAAAAGGC
  GACATTCAACAGTTTGGAAAATTATTAAGACAAATTCAAAAAAAGAAAAAGTGA
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⊟Protein

⊟General

• locus tag: NWMN_1366 [new locus tag: NWMN_RS07695 ]
• symbol: NWMN_1366
• description: DnaQ family exonuclease
• length: 897
• theoretical pI: 6.83206
• theoretical MW: 104217
• GRAVY: -0.297101

⊟Function

• reaction:
  EC 3.6.4.12?  ExPASy
  DNA helicase ATP + H₂O = ADP + phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 1269.1)
  and 6 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 139.1)
  Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 94.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 60.4)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair helicase (rad3) (TIGR00604; EC 3.6.4.-; HMM-score: 57.1)
  CRISPR type AFERR-associated DEAD/DEAH-box helicase Csf4 (TIGR03117; HMM-score: 47.5)
  Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 17.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  P-loop_NTPase (CL0023) Helicase_C_2; Helicase C-terminal domain (PF13307; HMM-score: 112.3)
  RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 91.3)
  and 5 more
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 22.8)
  Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 20.7)
  RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 18.7)
  no clan defined HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.6)
  DUF615; Protein of unknown function (DUF615) (PF04751; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002601
  • TAT(Tat/SPI): 0.00012
  • LIPO(Sec/SPII): 0.000314
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221578 NCBI
• RefSeq: YP_001332400 NCBI
• UniProt: A0A0H3K8N3 UniProt

⊟Protein sequence

• MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMIRTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKAFKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAKLMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQIIYRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEASLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALLIKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYFDQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEAHRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDIFGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIIDKLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1366 < birA < NWMN_1368 < NWMN_1369

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications