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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01474
  • pan locus tag?: SAUPAN003917000
  • symbol: SAOUHSC_01474
  • pan gene symbol?: papS
  • synonym:
  • product: tRNA CCA-pyrophosphorylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01474
  • symbol: SAOUHSC_01474
  • product: tRNA CCA-pyrophosphorylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1431599..1432801
  • length: 1203
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGT
    TTTGAAGCATATTATGTAGGTGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCAT
    GATATAGATATCACAACAAGTGCAACGCCGGATGAAATAGAATCTATCTTTAGTCATACG
    ATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
    GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACA
    TTTGTTCGTGATTTATACGAAGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCA
    ATGGATACAGCATACAAATTGTATGATTATTTTGATGGTCAACAAGATATTAATAATCGA
    ATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
    CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCG
    ATGCGTATACAAATGGCAGATATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTA
    ACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTAATCATTTAAAATCGCTGAAA
    GCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
    GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCA
    TTGAAGCCTTTAAAGCTAAGTAACCGACAAGTAAAAGATATCAATCAATATATTCAAATT
    ATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAAAAATGTTTGTTTATGATTAT
    GATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
    CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTA
    CCTATGCATAATAGAAAAGACATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAAT
    GCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACA
    GGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01474
  • symbol: SAOUHSC_01474
  • description: tRNA CCA-pyrophosphorylase
  • length: 400
  • theoretical pI: 5.1044
  • theoretical MW: 46435.9
  • GRAVY: -0.247

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.72?  ExPASy
    CCA tRNA nucleotidyltransferase A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate
  • TIGRFAM:
    poly(A) polymerase (TIGR01942; EC 2.7.7.19; HMM-score: 161.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification CCA tRNA nucleotidyltransferase (TIGR02692; EC 2.7.7.72; HMM-score: 161.1)
  • TheSEED  :
    • tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)
    RNA Metabolism RNA processing and modification tRNA nucleotidyltransferase  tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)
  • PFAM:
    NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 132.5)
    and 2 more
    HD_PDEase (CL0237) tRNA_NucTran2_2; tRNA nucleotidyltransferase domain 2 putative (PF13735; HMM-score: 84.5)
    no clan defined PolyA_pol_RNAbd; Probable RNA and SrmB- binding site of polymerase A (PF12627; HMM-score: 37)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9607
    • Cytoplasmic Membrane Score: 0.0335
    • Cell wall & surface Score: 0.0013
    • Extracellular Score: 0.0045
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004999
    • TAT(Tat/SPI): 0.000857
    • LIPO(Sec/SPII): 0.000527
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [4] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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