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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01389
  • pan locus tag?: SAUPAN003798000
  • symbol: SAOUHSC_01389
  • pan gene symbol?: pstS
  • synonym:
  • product: phosphate ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01389
  • symbol: SAOUHSC_01389
  • product: phosphate ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1331363..1332346
  • length: 984
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAAAATGGCAATTTGTTGGTACTACAGCTTTAGGTGCAACACTATTATTAGGTGCT
    TGTGGTGGCGGTAATGGTGGCAGTGGTAATAGTGATTTAAAAGGGGAAGCTAAAGGTGAT
    GGCTCATCAACAGTAGCACCAATTGTGGAGAAATTAAATGAAAAATGGGCTCAAGATCAC
    TCGGATGCTAAAATCTCAGCAGGACAAGCTGGTACAGGTGCTGGTTTCCAAAAATTCATT
    GCAGGAGATATCGACTTCGCTGATGCTTCTAGACCAATTAAAGATGAAGAGAAGCAAAAA
    TTACAAGATAAGAATATCAAATACAAAGAATTCAAAATTGCGCAAGATGGTGTAACGGTT
    GCTGTAAATAAAGAAAATGATTTTGTAGATGAATTAGACAAACAGCAATTAAAAGCAATT
    TATTCTGGAAAAGCTAAAACATGGAAAGATGTTAATAGTAAATGGCCAGATAAAAAAATA
    AATGCTGTATCACCAAACTCAAGTCATGGTACTTATGACTTCTTTGAAAATGAAGTAATG
    AATAAAGAAGATATTAAAGCAGAAAAAAATGCTGATACAAATGCTATCGTTTCTTCTGTA
    ACGAAAAACAAAGAGGGAATCGGATACTTTGGATATAACTTCTACGTACAAAATAAAGAT
    AAATTAAAAGAAGTTAAAATCAAAGATGAAAATGGTAAAGCAACAGAGCCTACGAAAAAA
    ACAATTCAAGATAACTCTTATGCATTAAGTAGACCATTATTCATTTATGTAAATGAAAAA
    GCATTGAAAGATAATAAAGTAATGTCAGAATTTATCAAATTCGTCTTAGAAGATAAAGGT
    AAAGCAGCTGAAGAAGCTGGATATGTAGCAGCACCAGAGAAAACATACAAATCACAATTA
    GATGATTTAAAAGCATTTATTGATAAAAATCAAAAATCAGACGACAAGAAATCTGATGAT
    AAAAAGTCTGAAGACAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01389
  • symbol: SAOUHSC_01389
  • description: phosphate ABC transporter substrate-binding protein
  • length: 327
  • theoretical pI: 8.71151
  • theoretical MW: 36117.2
  • GRAVY: -0.870031

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 344.1)
    and 2 more
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, phosphate-binding protein PstS (TIGR00975; HMM-score: 65)
    phosphate ABC transporter substrate-binding protein (TIGR04505; HMM-score: 50.1)
  • TheSEED  :
    • Phosphate ABC transporter, substrate-binding protein PstS
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)
  • PFAM:
    PBP (CL0177) PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 139)
    and 1 more
    PBP_like; PBP superfamily domain (PF12727; HMM-score: 25.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0005
    • Cytoplasmic Membrane Score: 0.8793
    • Cell wall & surface Score: 0.0324
    • Extracellular Score: 0.0879
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LLLGACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
    • SP(Sec/SPI): 0.000881
    • TAT(Tat/SPI): 0.0001
    • LIPO(Sec/SPII): 0.998765
    • Cleavage Site: CS pos: 20-21. LGA-CG. Pr: 0.9991
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSSTVAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQDKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKINAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQNKDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVLEDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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