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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01385
- pan locus tag?: SAUPAN003794000
- symbol: SAOUHSC_01385
- pan gene symbol?: pstB
- synonym:
- product: phosphate transporter ATP-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01385
- symbol: SAOUHSC_01385
- product: phosphate transporter ATP-binding protein
- replicon: chromosome
- strand: -
- coordinates: 1328429..1329280
- length: 852
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3920794 NCBI
- RefSeq: YP_499912 NCBI
- BioCyc: G1I0R-1295 BioCyc
- MicrobesOnline: 1289826 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGGCGCAAACACTTGCACAAACTAAACAAATATCTCAAAGTCATACGTTTGATGTCTCA
CAAAGTCATCATAAAACACCAGATGATACAAACTCACATTCTGTTATATATTCAACACAA
AATTTAGACTTATGGTATGGCGAAAATCATGCATTACAAAATATTAATTTAGATATTTAT
GAAAACCAAATTACTGCCATTATAGGTCCATCTGGTTGTGGCAAATCAACATATATTAAA
ACTTTGAATCGAATGGTTGAGTTAGTACCTTCTGTGAAAACAGCTGGTAAAATATTATAT
CGAGATCAAGACATTTTTGATCAAAAATATTCTAAAGAACAATTACGTACAAATGTGGGC
ATGGTCTTTCAACAACCTAATCCATTTCCAAAATCAATATACGATAATATTACTTACGGT
CCAAAGATTCACGGTATTAAAAATAAAAAAGTTCTTGATGAAATCGTTGAGAAATCATTA
CGTGGCGCTGCAATTTGGGATGAATTAAAGGATAGGTTGCACACAAATGCATATAGTTTA
TCCGGTGGGCAACAACAACGTGTTTGTATCGCGCGTTGTTTAGCAATTGAACCTGAAGTC
ATTTTAATGGATGAACCGACATCAGCATTAGATCCAATCTCAACATTAAGAGTAGAAGAG
TTGGTTCAAGAACTAAAAGAAAAGTATACAATTATTATGGTTACACATAATATGCAACAA
GCAGCTCGTGTATCAGATAAAACTGCATTTTTCTTAAATGGTTATGTCAATGAATATGAT
GATACTGATAAAATTTTCTCTAACCCATCAAACAAGAAAACAGAAGATTATATTTCAGGA
AGGTTTGGTTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01385
- symbol: SAOUHSC_01385
- description: phosphate transporter ATP-binding protein
- length: 283
- theoretical pI: 6.51418
- theoretical MW: 32154.1
- GRAVY: -0.537809
⊟Function[edit | edit source]
- reaction: EC 3.6.3.27? ExPASyPhosphate-transporting ATPase ATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In)
- TIGRFAM: Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 427)and 77 moreTransport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 172.3)Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 166.2)Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 150)ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 141.2)Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 136.1)Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 134.2)D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 126.6)thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 126.2)Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 125.1)Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 123.5)2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 118.9)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 118.8)Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 116.7)Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 116.5)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 115.5)Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 114.2)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 114.2)Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 112.9)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 109.1)Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 109)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 108.3)ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 106.7)ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 103.9)thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 102.3)Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 100.9)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 100.1)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 98.5)Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 96.7)Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 96.7)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 96.3)gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 89.1)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 86.6)Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 79.6)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 79.2)Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 77.9)proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 75.6)Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 74.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 69.6)phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 68.5)Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 67.6)Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 64.9)Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 59.2)ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 59.1)Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 57.8)Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 47.2)Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 43.9)DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 41.5)Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 39.7)Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 39.5)Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 39.5)Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 28.4)Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)DNA metabolism DNA replication, recombination, and repair checkpoint protein rad24 (TIGR00602; HMM-score: 21.4)Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 14.4)Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 13.9)Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 12.9)Ti-type conjugative transfer relaxase TraA (TIGR02768; HMM-score: 10.5)
- TheSEED :
- Phosphate ABC transporter, ATP-binding protein PstB
Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)and 1 more - PFAM: P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 119.1)and 31 moreAAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 44)SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 39.2)Rad17; Rad17 P-loop domain (PF03215; HMM-score: 23.4)AAA_23; AAA domain (PF13476; HMM-score: 20.3)AAA_22; AAA domain (PF13401; HMM-score: 20)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 19.9)AAA_15; AAA ATPase domain (PF13175; HMM-score: 18.4)AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.8)nSTAND1; Novel STAND NTPase 1 (PF20703; HMM-score: 17.5)RNA_helicase; RNA helicase (PF00910; HMM-score: 17.4)AAA_28; AAA domain (PF13521; HMM-score: 17.4)ABC_ATPase; P-loop domain (PF09818; HMM-score: 17.3)AAA_19; AAA domain (PF13245; HMM-score: 16.8)PRK; Phosphoribulokinase / Uridine kinase family (PF00485; HMM-score: 16.7)NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 16.6)AAA_18; AAA domain (PF13238; HMM-score: 16.2)AAA_14; AAA domain (PF13173; HMM-score: 15.4)ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.6)AAA_30; AAA domain (PF13604; HMM-score: 14.2)AAA_10; AAA-like domain (PF12846; HMM-score: 13.9)AAA_24; AAA domain (PF13479; HMM-score: 13.6)PhoH; PhoH-like protein (PF02562; HMM-score: 13.5)AAA_33; AAA domain (PF13671; HMM-score: 13.5)AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13)nSTAND3; Novel STAND NTPase 3 (PF20720; HMM-score: 12.8)Adeno_IVa2; Adenovirus IVa2 protein (PF02456; HMM-score: 12.7)Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 12.5)SpoIVA_ATPase; Stage IV sporulation protein A, ATPase domain (PF09547; HMM-score: 12.5)no clan defined VF530; DNA-binding protein VF530 (PF09905; HMM-score: 12.4)P-loop_NTPase (CL0023) AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 11.8)NB-ARC; NB-ARC domain (PF00931; HMM-score: 11.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.1268
- Cytoplasmic Membrane Score: 0.8385
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0346
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010288
- TAT(Tat/SPI): 0.000684
- LIPO(Sec/SPII): 0.001655
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDLWYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRDQDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSLRGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRVEELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTEDYISGRFG
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
SAOUHSC_02494 (rpsE) 30S ribosomal protein S5 [3] (data from MRSA252) SAOUHSC_01806 pyruvate kinase [3] (data from MRSA252) SAOUHSC_01814 hypothetical protein [3] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01384 < SAOUHSC_01385 < SAOUHSC_01386 < SAOUHSC_01387predicted SigA promoter [4] : SAOUHSC_01384 < SAOUHSC_01385 < SAOUHSC_01386 < SAOUHSC_01387 < SAOUHSC_01388 < SAOUHSC_01389
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 3.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)