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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01366
  • pan locus tag?: SAUPAN003762000
  • symbol: SAOUHSC_01366
  • pan gene symbol?: trpE
  • synonym:
  • product: anthranilate synthase component I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01366
  • symbol: SAOUHSC_01366
  • product: anthranilate synthase component I
  • replicon: chromosome
  • strand: +
  • coordinates: 1310437..1311843
  • length: 1407
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGATATATTTTATAAAAAAATAAAAGCAAATGTAACGCCCGAAGTTTTAGCACAACTT
    CATTCCAAGAAGATCATTTTGGAAAGTACAAATCAACAACAAACTAAAGGTCGCTATTCA
    GTTGTTATTTTTGATATTTATGGCACTTTAACTTTAGATAATGATGTATTATCAGTAAGT
    ACTTTAAAAGAATCGTATCAAATCACTGAAAGACCGTACCATTATTTAACGACTAAAATA
    AATGAAGACTACCATAATATTCAAGATGAGCAACTTAAGTCATTACCATTTATATCTGGA
    TATGTTGGGACGTGTAGCTTTGATTTAGTAAGACATGAATTTCCTAAATTGCAATCAATA
    CAATTAGAAGATCACAAGCAGCACGATGTAAGGTTATATATGGTTGAACAAGTTTATGTA
    TTTGACCATTACAAAGATGAGTTATATATCATCGCGACGAATCAATTTTCAAATTCAACA
    AAATCAGATCTTGAGAATCGAGTTATTAAGTCTATCGAAGACTTAACTAAAATCCAACCA
    TTCATGCCTACACAAGATTTTGATTTTAAAACTAAAGAAATTCAATCAAACATTTCTGAA
    GAAAGATTTATCGAAATGATTCAGTATTTCAAAGAGAAAATAACAGAAGGGGATATGTTC
    CAAGTTGTGCCATCAAGAATTTACAAATATGCGCATCATGCTAGTCAGCATTTAAATCAA
    CTTTCGTTTCAACTGTATCAAAATTTAAAACGACAAAACCCAAGTCCATATATGTATTAT
    CTTAATATCGATCAACCATATATTGTCGGTAGTTCTCCCGAAAGTTTCGTAAGTGTCAAA
    GATCAAATTGTAACAACTAATCCTATTGCAGGTACGATTCAACGTGGTGAGACGACACAA
    ATAGATAATGAGAATATGAAACAACTACTTAATGATCCAAAAGAATGCAGCGAACATCGT
    ATGCTAGTTGATTTAGGACGTAATGATATTCATAGAGTAAGTAAAATCGGTACCTCAAAA
    ATTACTAAATTAATGGTTATTGAAAAATATGAACATGTTATGCATATCGTAAGTGAAGTC
    ACAGGTAAAATAAATCAAAATTTATCGCCAATGACAGTTATTGCGAATTTATTACCAACA
    GGTACCGTTTCAGGTGCACCAAAATTACGTGCAATTGAAAGAATATATGAACAATATCCA
    CATAAACGGGGCGTTTATAGTGGTGGTGTTGGATACATAAATTGTAATCATAACTTAGAT
    TTTGCATTAGCAATTCGAACGATGATGATAGATGAGCAGTATATCAACGTAGAAGCTGGT
    TGTGGCGTTGTATATGATTCTATTCCTGAAAAAGAACTGAATGAAACGAAATTGAAAGCT
    AAAAGCTTATTGGAGGTGAGCCCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1407

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01366
  • symbol: SAOUHSC_01366
  • description: anthranilate synthase component I
  • length: 468
  • theoretical pI: 6.51458
  • theoretical MW: 54110.3
  • GRAVY: -0.45235

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.3.27?  ExPASy
    Anthranilate synthase Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 443.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 376.6)
    and 7 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 268.7)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 262.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 240.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 191.7)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 182)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 179.7)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 149.2)
  • TheSEED  :
    • Anthranilate synthase, aminase component (EC 4.1.3.27)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Tryptophan synthesis  Anthranilate synthase, aminase component (EC 4.1.3.27)
  • PFAM:
    no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 287)
    and 1 more
    Anth_synt_I_N; Anthranilate synthase component I, N terminal region (PF04715; HMM-score: 97.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002719
    • TAT(Tat/SPI): 0.000151
    • LIPO(Sec/SPII): 0.000515
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIFDIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYVGTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNSTKSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGDMFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESFVSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVSKIGTSKITKLMVIEKYEHVMHIVSEVTGKINQNLSPMTVIANLLPTGTVSGAPKLRAIERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPEKELNETKLKAKSLLEVSP

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: T-box(Trp) (transcription antitermination) regulon, CodY* (repression) regulon
    T-box(Trp)(5' cis-acting region)important in Amino acid metabolism; compare RegPrecise for N315 
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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