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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01364
  • pan locus tag?: SAUPAN003758000
  • symbol: SAOUHSC_01364
  • pan gene symbol?: tyrA
  • synonym:
  • product: prephenate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01364
  • symbol: SAOUHSC_01364
  • product: prephenate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1307658..1308749
  • length: 1092
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGACAACAGTTTTATTTGTTGGGCTTGGATTAATTGGTGGAAGTCTTGCTAGCAATATA
    AAATACCATAACCCTAATACTAATATTATTGCATACGATGCAGATATTTCTCAGTTAGAT
    AAAGCTAAATCAATCGGCATTATTAATGAAAAATGTTTAAATTATAGTGAAGCTATTAAA
    AAAGCCGATGTAATTATTTATGCAACACCTGTTGCTATCACAAATAAATATCTTAGCGAG
    CTTATAGATATGCCAACTAAACCTGGTGTTATTGTTTCTGATACTGGTAGTACTAAAGCA
    ATGATACAGCAACACGAATGCAATTTATTAAAGCATAATATTCATTTAGTCAGTGGTCAT
    CCAATGGCTGGTAGTCATAAATCTGGTGTACTAAATGCTAAAAAGCACTTATTTGAAAAC
    GCTTATTATATTTTAGTCTACAATGAGCCAAGAAATGAGCAAGCAGCAAACACGTTAAAA
    GAACTGTTATCACCTACTCTTGCTAAATTTATTGTAACTACTGCTGAAGAACACGACTAC
    GTAACAAGCGTCGTAAGTCATTTACCTCATATCGTTGCATCTAGTTTAGTTCATGTTAGT
    CAAAAGAACGGTCAAGAACATCATTTAGTTAATAAACTTGCAGCTGGTGGTTTTCGTGAT
    ATCACTCGTATAGCTAGTAGTAATGCACAAATGTGGAAAGATATCACCTTGAGTAATAAA
    ACGTATATTTTAGAAATGATTCGACAGCTAAAAAGTCAGTTTCAAGATTTAGAAAGACTA
    ATTGAAAGCAATGATTCTGAAAAATTGTTATCATTTTTTGCCCAAGCTAAATCGTATCGC
    GACGCACTACCCGCTAAACAACTAGGTGGACTAAATACTGCGTATGATCTATATGTAGAC
    ATTCCGGATGAATCAGGTATGATAAGTAAAGTGACTTATATCCTGAGTTTACATAACATA
    TCTATAAGCAACTTAAGAATCTTAGAAGTACGCGAAGATATATACGGTGCTTTAAAAATT
    AGTTTCAAAAACCCTACTGACCGAGAACGCGGTATGCAAGCATTGAGTGATTTTGATTGT
    TATATCCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01364
  • symbol: SAOUHSC_01364
  • description: prephenate dehydrogenase
  • length: 363
  • theoretical pI: 7.22968
  • theoretical MW: 40407
  • GRAVY: -0.153168

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.1.12?  ExPASy
    Prephenate dehydrogenase Prephenate + NAD+ = 4-hydroxyphenylpyruvate + CO2 + NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 26.7)
    and 5 more
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 20.6)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 17.9)
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 15.4)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 14.1)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 11.4)
  • TheSEED  :
    • Prephenate dehydrogenase (EC 1.3.1.12)
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  Prephenate dehydrogenase (EC 1.3.1.12)
    and 1 more
    Amino Acids and Derivatives Aromatic amino acids and derivatives Phenylalanine and Tyrosine Branches from Chorismate  Prephenate dehydrogenase (EC 1.3.1.12)
  • PFAM:
    NADP_Rossmann (CL0063) PDH; Prephenate dehydrogenase (PF02153; HMM-score: 230.7)
    and 7 more
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 31.1)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.4)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 20.4)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 18.9)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 16)
    GFO_IDH_MocA; Oxidoreductase family, NAD-binding Rossmann fold (PF01408; HMM-score: 15.6)
    no clan defined P4Ha_N; Prolyl 4-Hydroxylase alpha-subunit, N-terminal region (PF08336; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.37858
    • TAT(Tat/SPI): 0.003113
    • LIPO(Sec/SPII): 0.130738
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADISQLDKAKSIGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMIQQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLKELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGFRDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQAKSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDIYGALKISFKNPTDRERGMQALSDFDCYIQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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