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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01354
  • pan locus tag?: SAUPAN003744000
  • symbol: SAOUHSC_01354
  • pan gene symbol?: alsT
  • synonym:
  • product: sodium:alanine symporter family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01354
  • symbol: SAOUHSC_01354
  • product: sodium:alanine symporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1296849..1298309
  • length: 1461
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    TTGAAAGATTTCGATAGTTTAATACCTGGATGGTTTAAAGAATTTGTCCATGTTGGTACC
    GATTTAATATGGTCTCAATATTTAATTGGTCTATTATTGACAGCTGGATTCTTCTTTACG
    ATTAGTTCTAAATTCGTTCAATTACGAATGTTACCTGAAATGTTTAGAGCTTTAGTAGAA
    CGTCCAGAAACTTTAGAAGATGGTAAGAAGGGTATTTCGCCATTCCAAGCATTTGCGATT
    AGTGCTGGTTCGAGAGTTGGTACTGGTAATATTGCTGGTGTTGCGACTGCGATTGTTTTA
    GGCGGTCCAGGTGCAGTATTTTGGATGTGGGTTATTGCATTTATAGGTGCAGCGAGTGCA
    TTTATAGAAGCGACTTTGGCTCAGGTTTATAAAGTACATGATAAAGATGGTGGATTCCGT
    GGTGGTCCAGCTTACTATATTACTAAAGGTTTAAATCAAAAATGGCTAGGTATCGTATTT
    GCGATTTTAATTACAATTACATTTGCATTTGTATTTAACACAGTGCAATCTAATACAATT
    GCGGAGTCGTTAAATACGCAATATAATATTAGTCCAGTAATCACAGGTATTATTTTAGCA
    ATCGTAACAGCTATTATTATATTTGGTGGTGTACGTAGTATTGCTACGTTATCTTCGTTA
    ATTGTACCGATTATGGCTATCATTTACATTGGTATGGTTTTAGTAATATTGCTATTTAAT
    TTAGATCAAATTGTTCCTATGATAGGTACGATTATTAAAAGTGCATTTGGTATCGAACAA
    GTAACTGGTGGCGCTGTAGGTGCTGCGGTTCTTCAAGGTATCAAACGTGGTTTATTCTCT
    AACGAAGCTGGTATGGGTTCTGCGCCGAATGCAGCGGCAACTGCTGCCGTACCACACCCT
    GTTAAGCAAGGTTTAATCCAATCATTAGGTGTGTTCTTTGATACAATGTTGGTTTGTACA
    GCAACTGCAATCATGATTTTACTATATTCAGGACTGAAATTTGGTGATAACGCACCTCAA
    GGTGTTGCAGTTACTCAATCAGCACTTAATGAGCATTTAGGTTCTGCTGGAGGTATTTTC
    TTAACAATAGCAGTTACACTGTTTGCATTTTCATCTGTTGTAGGTAATTACTATTACGGT
    CAATCTAATATTGAATTTTTATCAACAAACCGTGTAATATTATTTATCTTTAGATGTCTT
    GTTGTAGTACTTGTCTTTGTCGGTGCAGTTGTAAAAACAGAAACAGTATGGAATACGGCA
    GACTTATTTATGGGCTTAATGGCAATTGTAAACATTATTTCCATTATAGGACTGTCCAAT
    GTAGCTTTTGCATTGATGAAAGATTATCAAAAGCAGAAAAAAGAAGGCAAGAACCCTGTC
    TTTAAACCTGAAAACTTAGAAATTAACTTATTTGGAATTAGTGCTTGGGGCGCTAACAAA
    TATAAGAACTCTGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1461

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01354
  • symbol: SAOUHSC_01354
  • description: sodium:alanine symporter family protein
  • length: 486
  • theoretical pI: 9.31622
  • theoretical MW: 52141
  • GRAVY: 0.687654

⊟Function[edit | edit source]

  • TIGRFAM:
    amino acid carrier protein (TIGR00835; HMM-score: 508.5)
    and 2 more
    phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 16.7)
    exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 5.8)
  • TheSEED  :
    • Sodium:alanine symporter family protein
  • PFAM:
    APC (CL0062) Na_Ala_symp; Sodium:alanine symporter family (PF01235; HMM-score: 544.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001736
    • TAT(Tat/SPI): 0.000269
    • LIPO(Sec/SPII): 0.001098
  • predicted transmembrane helices (TMHMM): 9

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSKFVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGGPGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVFAILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLSSLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKRGLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKFGDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRVILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQKQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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